FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005202933

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202933
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5451501
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTACAGCATCTCGTATGC63040.11563787661416554TruSeq Adapter, Index 7 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG8950.030.72247543
CGTATGC8900.030.1541444
GATCGGA9550.029.2953931
CTCGTAT9400.028.78500642
TACGGCG6150.028.2566816
TCTCGTA9900.027.33372741
TGATACG5550.026.9516333
ATACGGC6300.026.187255
ATCGGAA10950.025.7137262
ATCTCGT10700.025.70177540
ACACGTC12450.022.96905713
ACGTCTG13000.021.82808115
GTCACTA13500.020.69697429
CACGTCT14000.020.2689314
GATACGG8050.020.2211114
CGTCTGA14700.019.7526716
ACGGCGA8650.019.5814027
CACACGT15100.019.0837412
GCACACG15900.018.40024811
TCACTAC14950.018.39626330
Produced by FastQC (version 0.11.5)