FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005202934

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202934
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3690705
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGAAGATCTCGTATGC41790.11323039907009637TruSeq Adapter, Index 10 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG6900.030.9246543
CTCGTAT7000.030.48328442
CGTATGC7050.030.2666844
TCTCGTA7150.029.84539241
GATCGGA7850.029.7526051
ACACGTC7900.028.68086813
TACGGCG4050.028.244286
TGATACG4100.027.36333
CACTCGA8050.026.78555331
ATCGGAA8950.026.0534362
ACTCGAA8650.025.43632132
ATACGGC4550.025.140515
CACACGT9800.023.34476312
TCACTCG9550.023.03793330
ATCTCGT9600.022.68691840
CACGTCT9900.022.6645514
ACGTCTG10000.022.21792615
GATACGG5100.021.9979484
TCGGAAG10850.021.4910813
CGTCTGA10750.021.28231416
Produced by FastQC (version 0.11.5)