FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005202935

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202935
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3704918
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGAAGATCTCGTATGC42230.11398362932728875TruSeq Adapter, Index 10 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG6350.032.91038543
CGTATGC6500.032.82822444
CTCGTAT6850.031.15044842
GATCGGA7250.029.783861
TACGGCG4750.028.7131736
ATCGGAA7750.028.1006662
TGATACG4550.028.0413993
ATACGGC4900.027.385265
TCTCGTA8000.026.95154641
ACACGTC8500.026.39759413
CACTCGA8450.026.037731
ACTCGAA8800.024.50206632
GATACGG5800.024.2736514
ACGTCTG9300.023.89029715
CGTCTGA9650.023.0238116
ACGGCGA6400.021.6542767
TCGGAAG10250.021.461463
ATCTCGT10050.021.45396840
CACACGT10700.021.1756412
CACGTCT10950.020.29038814
Produced by FastQC (version 0.11.5)