FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005202936

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202936
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3692675
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGAAGATCTCGTATGC44140.11953394219637525TruSeq Adapter, Index 10 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATACG4300.034.276523
CGTATGC5850.031.5878244
TACGGCG4750.031.4923926
CTCGTAT5950.031.05692942
ATACGGC4900.030.9772835
TCGTATG6150.030.40423843
GATCGGA6450.029.3729081
GATACGG5700.025.8577234
ATCGGAA7350.025.4402832
ACACGTC7850.024.10011513
TCTCGTA7600.024.0271341
CACTCGA7800.023.69246731
ACTCGAA8100.022.81558832
CACACGT8400.022.78401812
CGTCTGA8600.022.50995316
ACGGCGA6850.022.4800787
ACGTCTG8450.022.1285315
GCGACCA7350.021.25011810
TCACTCG9100.020.3078330
ATCTCGT9250.020.21717540
Produced by FastQC (version 0.11.5)