FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005202937

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202937
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3691371
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGAAGATCTCGTATGC41860.11339960139471217TruSeq Adapter, Index 10 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG6250.034.14038543
CGTATGC6500.033.16661544
GATCGGA6600.032.0443571
TGATACG4400.030.496773
TACGGCG4750.030.1020746
CTCGTAT7000.029.85600342
ACACGTC7700.027.7113513
ATACGGC5100.027.604925
TCTCGTA7700.027.4290141
ACGTCTG8450.026.553415
ATCGGAA7900.026.4528942
CACTCGA8050.025.69089731
CACGTCT9050.024.79295514
ACTCGAA8350.024.50438332
ATCTCGT8700.024.27624940
CACACGT9450.022.81239912
GATACGG6500.021.6592434
TCACTCG10050.021.01611730
CGTCTGA10300.020.71625316
CGAAGAT10550.019.81208635
Produced by FastQC (version 0.11.5)