FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005202938

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202938
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5731859
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATACG7450.031.2990153
ATACGGC8300.029.9489385
TACGGCG9050.027.2239116
GATACGG9250.026.6352844
TCGTATG6900.023.91117343
CGTATGC7050.023.71445744
GCGACCA11450.021.9018310
CTCGTAT7500.021.70553642
ACGACGC5700.021.22807541
ATCTCGT8900.020.5168640
ACGGCGA12650.020.1719827
TCTCGTA8850.019.88700941
GATCTAC12500.019.88696321
AATCTCG8600.019.44186439
GATCGGA8650.018.8490981
TACACGA7300.018.68575138
ATGATAC12700.018.5336592
CGGCGAC14000.018.3839538
ACACGTC9100.018.37186613
ACCACCG14200.017.81519513
Produced by FastQC (version 0.11.5)