FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005202940

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202940
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5735269
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATACG8000.033.546813
ATACGGC8950.030.7233355
TACGGCG9550.028.7930746
TCGTATG7200.026.88633343
CGTATGC7250.026.70114144
GATACGG11050.024.884514
CTCGTAT8000.023.92376742
ATGATAC12000.022.5478552
GCGACCA13000.021.82869110
CGACGCT5750.021.42498442
ACGACGC6400.021.31344641
CGGCAAT8850.021.13180435
ACCGAGA13700.020.87392616
GATCGGA9400.020.1584321
ACGGCGA14150.020.054637
GATCTAC13900.019.78349921
ACACGTC9950.019.6765213
AATCTCG9600.019.48087939
TCTCGTA9800.019.3069841
ATCTCGT10250.019.10378840
Produced by FastQC (version 0.11.5)