FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005202941

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202941
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5724091
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACGGC8850.030.8205285
TACGGCG8900.030.4002196
TGATACG9050.029.167173
TCGTATG6050.026.54195443
GATACGG10450.025.68064
CTCGTAT6700.023.6401242
CGTATGC6900.023.27270144
ACGACGC5400.021.59370841
ACGGCGA13050.021.4069527
GCGACCA12900.021.31483510
ACACGTC7950.021.3051813
GATCGGA8100.020.9406411
CGACGCT5550.020.61115642
ATGATAC12750.020.5304492
GATCTAC13600.020.05898321
GGCGACC14150.019.8982649
AATCTCG8550.019.04325139
ATCTCGT8600.018.93137640
CGGCGAC14950.018.8334718
TCTCGTA8750.018.35523241
Produced by FastQC (version 0.11.5)