FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005202942

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202942
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7538296
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA205010.27195801279228093No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACGGC30550.037.8039865
TACGGCG30950.037.4575586
TGATACG30800.037.2828643
GATACGG32100.036.047094
ACGGCGA33300.034.946287
GCGACCA35500.032.84256410
GATCTAC36500.030.97927521
ATGATAC37400.030.7623562
CGACCAC37750.030.71023611
ACCGAGA38050.030.2946616
ACCACCG38350.030.28712313
ACGACGC14300.030.1543441
CGGCGAC39100.029.8749548
GGCGACC40050.029.2761659
CCACCGA39950.028.79880114
AGATCTA40550.028.21067620
CGACGCT14450.028.16468442
TACACGC15550.027.86943825
TCGTATG10500.027.44588743
TCTACAC42750.026.6559823
Produced by FastQC (version 0.11.5)