FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005202943

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202943
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7567092
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA211240.279156114396389No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATACG31350.039.9963653
ATACGGC32100.039.9527555
TACGGCG33000.038.7298166
GATACGG34900.036.7473754
GCGACCA37250.034.90152710
ACGGCGA38050.034.109917
GATCTAC38000.033.0553321
ACCACCG41550.031.34251813
CGACCAC41550.031.23663111
ATGATAC40700.031.0242062
CCACCGA41950.030.99122214
ACCGAGA41700.030.96621516
CGGCGAC42350.030.9062868
TACACGA18150.030.54772638
ACGACGC17250.030.35547841
CGACGCT16500.030.13139342
GGCGACC43150.030.0273979
AGATCTA42100.029.88841420
TACACGC16050.028.91968225
TCTACAC43650.028.87729323
Produced by FastQC (version 0.11.5)