FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005202944

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202944
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7549275
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA218430.2893390424908352No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATACG33150.039.881163
ATACGGC33650.039.811555
TACGGCG34400.038.7517246
GATACGG35850.037.184364
ACGGCGA38350.035.3913087
GCGACCA39500.034.1938610
GATCTAC39950.033.26004821
CGGCGAC41500.032.8109938
ATGATAC41150.032.3951232
CGACCAC42600.031.70557211
ACCGAGA42600.031.60229716
GGCGACC43250.031.4325229
CCACCGA43400.031.27319714
ACCACCG43750.031.17385313
AGATCTA44550.029.92375820
TACACGC17650.029.78939625
CACCGAG46550.029.25147815
ACGACGC14500.028.98182141
TCTACAC46150.028.93494223
CGAGATC46850.028.8767418
Produced by FastQC (version 0.11.5)