FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005202945

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202945
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7546477
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA214620.2843976069893276No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATACG32500.039.6635743
TACGGCG33300.039.437356
ATACGGC33450.039.1947365
GATACGG36050.036.7340554
ACGGCGA38500.034.796397
GCGACCA38700.034.61656610
GATCTAC39150.033.3225321
ACCGAGA40650.032.9018816
CCACCGA41150.032.71592714
ACCACCG41900.032.0778213
CGGCGAC42050.032.01578
CGACCAC41950.031.82983211
ATGATAC40800.031.756512
GGCGACC43100.031.1846989
AGATCTA42250.030.92942820
CACCGAG44700.030.26531615
TCTACAC43900.029.91746923
ACGACGC16600.029.02377741
CGACGCT16100.028.9682442
CGAGATC46700.028.45138518
Produced by FastQC (version 0.11.5)