FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005202946

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202946
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6278193
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCT126410.20134774448635143Illumina Single End PCR Primer 1 (100% over 50bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA97970.15604808581067833No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATACG66000.041.663283
ATACGGC69150.041.1651275
TACGGCG69400.040.985146
GATACGG70300.040.523024
ACGGCGA73750.039.1046187
GCGACCA74700.038.51895510
ACGACGC32500.038.51655241
TACACGA33300.038.0558138
GATCTAC74050.038.02587521
ATGATAC72500.038.018982
CCACCGA75850.037.76093714
ACCGAGA76000.037.5709316
ACACGAC33000.037.53297439
CGGCGAC77250.037.4752738
CGACCAC76650.037.42422511
CACGACG33450.037.3568940
CGACGCT31350.037.26042642
GGCGACC78150.036.9311039
ACCACCG77300.036.91032413
AGATCTA76850.036.75491720
Produced by FastQC (version 0.11.5)