FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005202947

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202947
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6304388
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCT177410.2814071722742953Illumina Single End PCR Primer 1 (100% over 50bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA99750.1582231296677806No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATACG71000.041.9816863
TACGGCG73150.041.4093636
ATACGGC73300.041.26465
GATACGG73300.041.204584
ACGGCGA78650.039.0729877
GCGACCA78900.038.86553610
ATGATAC76600.038.8263742
ACGACGC36200.038.77695541
GATCTAC77950.038.54964421
TACACGA37700.038.46028538
ACACGAC37150.037.84517739
CCACCGA81000.037.80359614
CGACCAC81150.037.78793311
GGCGACC81550.037.683519
CGGCGAC81600.037.6334578
ACCGAGA81700.037.587416
ACCACCG81650.037.5295913
AGATCTA81350.037.1007220
CGACGCT35950.036.83751342
TCTACAC82400.036.44108223
Produced by FastQC (version 0.11.5)