FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005202948

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202948
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6284950
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCT161560.25705852870746787Illumina Single End PCR Primer 1 (100% over 50bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA103610.16485413567331483No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGCG75750.041.963246
TGATACG75050.041.768413
ATACGGC76950.041.337435
GATACGG77950.040.8635644
ACGGCGA80600.040.011317
GCGACCA81900.039.42992410
ACGACGC33900.038.94045341
ATGATAC80750.038.8472982
GATCTAC81750.038.69685421
CGACGCT32300.038.3436242
GGCGACC84350.038.23259
CCACCGA85050.037.866114
TACACGA37100.037.8361238
ACACGAC35850.037.80518339
ACCACCG85400.037.7881913
CGGCGAC85800.037.6889348
CGACCAC85900.037.54262511
ACCGAGA85800.037.48382616
CACGACG35600.036.83461440
AGATCTA86550.036.62613720
Produced by FastQC (version 0.11.5)