FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005202949

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202949
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6280097
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCT157960.25152477740391593Illumina Single End PCR Primer 1 (100% over 50bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA100710.16036376508197248No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGCG74500.042.2816966
TGATACG73150.042.2200163
ATACGGC74800.042.0827065
GATACGG76850.041.0460054
ACGGCGA79950.040.142327
ATGATAC77750.039.75042
ACGACGC35800.039.57715241
GCGACCA82050.039.0612910
GATCTAC80850.038.7741221
GGCGACC83250.038.6303639
CCACCGA83600.038.46862814
CGACGCT35000.038.21516442
TACACGA38750.038.0437138
CGACCAC84600.037.93591311
CGGCGAC85200.037.7462168
ACACGAC38100.037.5954939
ACCACCG85650.037.49653613
ACCGAGA85750.037.47845516
AGATCTA84550.037.10304620
TACACGC32450.036.88003525
Produced by FastQC (version 0.11.5)