FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005202951

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202951
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3356985
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGTTA3450.029.33885433
TCGTATG3500.028.28176743
CTCGTAT3700.026.7546242
ACCGGTT3900.025.95321832
CGGTTAA3850.025.71912834
CGTATGC4200.024.09259644
GATCGGA4600.022.9858461
TACGGCG1650.022.6631686
ACACGTC5150.021.78304513
TCTCGTA5250.019.69806741
TGATACG1900.019.6811733
ACGTCTG5800.019.3418415
ATACGGC1950.019.1765275
ATCGGAA5650.019.0767022
AATCTCG5250.018.86013239
TCACCGG5550.018.63358530
ATCTCGT5500.018.40264140
CACGTCT6600.017.66393914
GTCACCG6250.017.60252629
CGTCTGA6650.016.87007916
Produced by FastQC (version 0.11.5)