FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005202953

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202953
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3346170
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG3050.025.96343
CGTATGC3050.025.24255844
CCGGTTA2850.024.70586633
ACCGGTT3000.024.20185732
TGATACG2200.022.9962653
CGGTTAA3100.022.71345734
CTCGTAT3300.022.6646842
ATACGGC2400.021.996435
ACACGTC4050.020.63862413
GATCGGA4300.018.4437261
ATCGGAA4550.017.8872052
CGAATCG750.001292924217.5971413
TACGGCG3050.017.3086666
TCTCGTA4500.017.11165841
ACGTCTG4800.016.95558215
AATCTCG4700.016.38570639
GATACGG3250.016.2435174
ATCTCGT5200.014.38550440
GCCGAAT2305.838956E-1014.3673941
TCACCGG5400.014.26035530
Produced by FastQC (version 0.11.5)