FastQCFastQC Report
Wed 8 May 2024
EGAF00005202955

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202955
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1819561
Total Bases90.9 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCT46290.2544020233451915Illumina Single End PCR Primer 1 (100% over 50bp)
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC45790.25165410777654607No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30650.16844722435796325No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAAGAAATCTCGTATGC26460.14541969189271478TruSeq Adapter, Index 3 (97% over 37bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)