FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005202955

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202955
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1819561
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCT46290.2544020233451915Illumina Single End PCR Primer 1 (100% over 50bp)
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC45790.25165410777654607No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30650.16844722435796325No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAAGAAATCTCGTATGC26460.14541969189271478TruSeq Adapter, Index 3 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATACG21350.041.728673
GATACGG21850.041.6798634
ATACGGC21950.041.489985
TACGGCG22250.040.930566
GCGACCA23200.040.0130710
ACGGCGA23200.039.918257
ACGACGC9700.039.46739641
ATGATAC22700.039.247012
GATCTAC23350.039.19077321
CGGCGAC23800.039.0043338
ACCACCG24050.038.59888513
TACACGA10100.038.5578538
CGACCAC24400.038.1353711
CCACCGA24550.037.90236314
CGACGCT9850.037.52202642
ACCGAGA24900.037.36959516
GGCGACC25000.037.3081059
CACGACG10350.037.20134440
AGATCTA24900.036.75118620
TCTACAC24950.036.58936723
Produced by FastQC (version 0.11.5)