FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005202957

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202957
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1814331
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC47540.2620249557550414No Hit
AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCT38520.21230966124703818Illumina Single End PCR Primer 1 (100% over 50bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA31520.17372794710557224No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAAGAAATCTCGTATGC25990.14324839293381417TruSeq Adapter, Index 3 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACGGC21750.042.073055
TGATACG21250.042.0278363
TACGGCG22050.041.4008646
GATACGG22100.041.30724
ACGGCGA23150.039.8137327
ATGATAC23000.038.8300672
GATCTAC23350.038.8175521
GCGACCA23800.038.72638310
CGGCGAC24100.038.2443168
CGACCAC24300.038.02007311
CGACGCT7950.037.9094342
CCACCGA24650.037.4802314
ACCGAGA24750.037.41767516
ACGACGC8900.037.0782341
AGATCTA24700.037.0522120
ACACGAC8850.037.04321739
GGCGACC25100.036.7206349
ACCACCG25300.036.517313
TCTACAC24850.036.47443823
TACACGA9250.036.3927938
Produced by FastQC (version 0.11.5)