FastQCFastQC Report
Thu 21 Jul 2022
EGAF00005203538

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005203538
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences101178806
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTACTTTCAACAAAATCAGCAGCTAATGAAGGCAAGTCAGCAGGCATCT3569100.35275174130835263No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTATG2787040.2754568975641005TruSeq Adapter, Index 1 (97% over 35bp)
CTCCTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTCTA2614630.2584167676380763No Hit
CTTCAGTTTACTGGAGATCGAATCTTGATTTTCACAGATATACTTGGCAA2391760.2363894272482322No Hit
CTCTTTTGTTGCCTTGGGCTTGTGTTTCACAAGCTCAACAAGTGCAGTTT2327480.23003631808029046No Hit
CTCTGTTTGGCAGACGAAGCCTTCCCTTCATCCCGAAGTTCATCGAGCTT2265340.22389471565813893No Hit
CTCTTTTGTTGCCTTGGGCTTGTGTTTCACGAGCTCAACAAGTGCAGTTT2166940.21416935874890636No Hit
CCCAAATCTTTAAACCGATGAGCAACCTCACTCTTGTGTGCATCTCGACG1623630.1604713540501753No Hit
CTCTTATTCTCATGGTAGGCTGAGATGCTTTTAAATGTGATGTTATAAGC1517760.15000770022923576No Hit
CTTCATTGTCATGAAAAGCAGTGCACATCACATCAACCTCTGGTCTCACC1471560.14544152655843753No Hit
CTCAGCTTTGGGAAATCTCTGGCTCAGGCGAGCTACTGCCCATGCTTTGA1340940.13253170827099897No Hit
CTTGATTTGTCTCTCCTTCTCAGAAAGTGTGCATATATCTGCATGGAAGG1242000.12275298050067916No Hit
CTCCTTATCGTCAGCCTTGCAGCACTTCTCTACAAAAGCTGCGAAATCAT1168040.11544314923028445No Hit
CCCACTTTTCCTAGGTTTCTTGAGACCTCTACAAGAGTTGGAGTTGACAC1144420.11310866823235688No Hit
CTTCAGGATGTTTACAACATTTGCTGCCCACTTTTCCTAGGTTTCTTGAG1112880.10999141460514962No Hit
CTCAGGTTCTTGTTTTGCACAGCAGTCAGCCATTTCACCACAGGTTTCAC1107840.10949328656833528No Hit
CGCCTGTTCACCAAGGATTCTGTGCAGCATTTGGTGACTCTGTCACTTAC1074140.10616254949677899No Hit
CTCAGGTTCTTGTTTTGCACAGCAGTCAGCCATTTCACCATAGGTTTCAC1067900.10554581954643744No Hit
CTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTCTACAA1017230.10053785374775029No Hit
CTCTTCATTGTCATGAAAAGCAGTGCACATCACATCAACCTCTGGTCTCA1017170.10053192365207393No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)