FastQCFastQC Report
Thu 21 Jul 2022
EGAF00005203539

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005203539
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences101178806
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGTCCTGGTGTAGATCTC3224450.31868828339405386Illumina Single End PCR Primer 1 (96% over 32bp)
CCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTT1623690.16047728414585166No Hit
CTTTTCTTTGCTAAAAGGTATAAAGCTGCTTTTACAGAATGTTGCCAAGC1353470.13377010991807908No Hit
CTCCTTTTCTTTGCTAAAAGGTATAAAGCTGCTTTTACAGAATGTTGCCA1275190.12603331175898635No Hit
CTGAAACATTCACCTTCCATGCAGATATATGCACACTTTCTGAGAAGGAG1274460.12596116226159065No Hit
CTTACTTTTATGCCCCGGAACTCCTTTTCTTTGCTAAAAGGTATAAAGCT1173620.11599464812818605No Hit
CCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTT1110760.1097818845579182No Hit
CTTTATTTCCCTTCTTTTTCTCTTTAGCTCGGCTTATTCCAGGGGTGTGT1092190.10794651994608437No Hit
GTTTCGTCGAGATGCACACAAGAGTGAGGTTGCTCATCGGTTTAAAGATT1023650.10117237398512095No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)