FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005203600

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005203600
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46198130
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT2049380.44360670009803427No Hit
CTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAG1547440.3349572807384195No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA513120.11106943073236947No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG478460.10356696255887413No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC414250.024.653454
CAGACCG421350.024.3555833
GACCGCG427250.023.8880355
ACCGCGT444800.022.9402286
TCAGACC474850.022.0995082
CTCAGAC531700.020.626821
CCGCGTT506150.020.1591177
CGCGTTC527500.019.3601978
GCGTTCT582800.017.5579159
CTACACG145800.016.71190845
CGTTCTC639300.016.42838710
CCGTACG136150.014.9888974
CGTACGC140200.014.5883215
TCCGTAC146650.014.2072613
CGGCAGT544650.014.19774840
AGTTGCA542650.014.12148744
TACGCCA145950.014.120987
GTTCTCT757200.014.10216811
ACGGCAG565250.013.680325539
TTCGGCG150100.013.62507244
Produced by FastQC (version 0.11.5)