FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005203602

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005203602
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52609331
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT729250.13861609454794246No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC201500.020.1852914
CAGACCG211750.019.3250643
TCACGCT204900.018.4330718
GAACGAT224300.018.32472445
GTCCGTT39250.017.7443181
GACCGCG238500.017.1292825
AACGCTA214400.016.9558523
ACGCTAC217550.016.73226224
ACCGCGT249750.016.4117366
CGTTACG23850.016.22293721
ACGCTCA244050.016.1120810
TTCACGC236450.016.0686477
CCGCGTT256600.016.0341537
CGCTACC226650.016.02075625
CGCGTTC259000.015.9029458
GCGTTCT262050.015.657769
TTCGACG16000.015.6068296
AAAACGC234650.015.53044321
GCCGGTT92700.015.3909171
GAAAACG239350.015.21606620
Produced by FastQC (version 0.11.5)