FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005203606

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005203606
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50765058
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT634220.12493238951879065No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC142750.021.6569184
CAGACCG152050.020.5238953
GAACGAT145000.020.3580245
TTCGGCG82400.019.2235544
GACCGCG163250.018.90985
ACCGCGT166850.018.5692236
CCGCGTT173150.017.9065827
CGCGTTC175200.017.6842178
GTCCGTT36850.017.0460761
AGAACGA176350.016.85448644
CGTTACG18150.016.73494521
AATACGG181250.016.23733932
CCGTACG97700.016.2360574
ATACGGA182050.016.21542433
CGTACGC99850.015.9991265
TACGGAG187600.015.7596934
TCGGCGC102400.015.578155545
CCCGTAT30700.015.5473091
TCAGACC207350.015.3973352
GCGTTCT203850.015.3091749
Produced by FastQC (version 0.11.5)