FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005203607

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005203607
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50765058
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG144650.013.92125245
ATAGGGG155300.012.95206238
GGCATAG153700.012.82342335
GGGAGGT240400.012.32605653
GAGCTTA170400.011.89674244
CATAGGG171050.011.7331537
TGGCATA178300.011.09203634
GCATAGG182000.010.97777836
GGAGGTC266300.010.8399764
TCAGACG82300.010.5799592
ACCCGGC66200.010.4340585
GTTGTCG18450.010.36562326
TAGGCTA85000.010.2968310
TATCCTA94500.09.8093055
ACTCTCC286300.09.784111
GTCAGGA162300.09.519351
ATCCTAG112000.09.4819326
GTCACTC298450.09.3857858
GGTCACT292950.09.331597
GTATCCT106100.09.2669944
Produced by FastQC (version 0.11.5)