FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005203655

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005203655
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17386168
Sequences flagged as poor quality0
Sequence length35-51
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAACCTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGT1188950.6838482177326252No Hit
CTCAGACTTTATTCAAAGACCAGGAAGGGCCGGTGCAAGGAGGGGAGGAGG697540.40120399158687525No Hit
CTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTC513600.2954072455759084No Hit
CGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAAC495090.28476085126981404No Hit
CTCGTACTGAGCAGGATTACCATGGCAACAACACATCATCAGTAGGGTAA427480.2458736163138421No Hit
CTGACTTAGAGGCGTTCAGTCATAATCCCACAGATGGTAGCTTCGCCCCAT410430.2360669700189254No Hit
CCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTC410250.23596343944220485No Hit
CCTTCCGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTACTTCCTCT403910.23231686246215957No Hit
CTCTAATCATTCGCTTTACCGGATAAAACTGCGTGGCGGGGGTGCGTCGGG365810.21040289038964768No Hit
CTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGGACGCCGACCGCTCGGGG360770.20750403424147287No Hit
CCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTTC351920.20241378088604686No Hit
CTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTCCGCC338980.1949710827595822No Hit
CCTCAGCCAAGCACATACACCAAATGTCTGAACCTGCGGTTCCTCTCGTAC338040.1944304230811528No Hit
CTAGAGTCAAGCTCAACAGGGTCTTCTTTCCCCGCTGATTCCGCCAAGCCC320740.18447998431856866No Hit
ACCGAGGCTCCGCGGCGCTGCCGTATCGTTCCGCCTGGGCGGGATTCTGAC306840.17648512311626116No Hit
CTTCGCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCT303940.1748171304913193No Hit
CTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGG301740.17355175677584617No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGAC297770.17126833238928785No Hit
CGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTT289380.16644265717436987No Hit
CTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATC282960.16275006660467103No Hit
CAAAGACTTTGGTTTCCCGGAAGCTGCCCGGCGGGTCATGGGAATAACGCC274980.1581602110367276No Hit
CTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCG267230.15370264453903817No Hit
CTCAGCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGC263250.15141346845377313No Hit
CTTCCGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTACTTCCTCT259480.14924507804134873No Hit
CGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTT242770.13963398950245967No Hit
CGAGGCTCCGCGGCGCTGCCGTATCGTTCCGCCTGGGCGGGATTCTGACTT241810.13908182642661684No Hit
CACAGATGGTAGCTTCGCCCCATTGGCTCCTCAGCCAAGCACATACACCA240500.13832835389603967No Hit
CCGAGGCTCCGCGGCGCTGCCGTATCGTTCCGCCTGGGCGGGATTCTGACT235180.13526845018407735No Hit
CTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGCCGACTTCCC229810.1321797879785816No Hit
CAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGT220980.127101038020569No Hit
CTTAGAGGCGTTCAGTCATAATCCCACAGATGGTAGCTTCGCCCCATTGGC217370.12502467478745172No Hit
GTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGC217300.12498441289650485No Hit
CCCGAAGTTACGGATCCGGCTTGCCGACTTCCCTTACCTACATTGTTCCA209980.12077416944320336No Hit
CGAAGGATCAAAAAGCGACGTCGCTATGAACGCTTGGCCGCCACAAGCC207020.11907166662602134No Hit
CGCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTC206930.11901990133766106No Hit
CTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAAGGATCGTGAGG206280.11864604092172583No Hit
CGAAACCCCGACCCAGAAGCAGGTCGTCTACGAATGGTTTAGCGCCAGGTT202050.1162130723687934No Hit
CGCAGGGCCGGCGGACCCCGCCCCGGGCCCCTCGCGGGGACACCGGGGGGG199090.11451056955161136No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGAC197690.11370533173267394No Hit
CATAATCCCACAGATGGTAGCTTCGCCCCATTGGCTCCTCAGCCAAGCAC197630.11367082154043376No Hit
CCTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCG185740.10683205177817216No Hit
CACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACCACC184540.10614184793336864No Hit
CAACAACACATCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTA181660.10448535870584018No Hit
CTGGAGGATCGCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCG180480.10380665825845004No Hit

[OK]Adapter Content

Adapter graph