FastQCFastQC Report
Thu 2 May 2024
EGAF00005203676

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005203676
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences99156115
Total Bases5 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTGGTGGTGGGGAAGGACAGGAACATCCTCTCCAGGGCCTCCGCACC10700451.079151800168855No Hit
CTCAGGTCGAAGTGCGGGAAGTAGGTCTTGGTGGTGGGGAAGGACAGGAA7607420.767216424322393No Hit
CTGGGCAGAGCCGTGGCTCAGGTCGAAGTGCGGGAAGTAGGTCTTGGTGG7518760.7582749687197808No Hit
CTTGGTGGTGGGGAAGGACAGGAACATCCTCTCCAGGGCCTCCGCACCAT6692620.6749578682061111No Hit
CTTTAATAGAAATTGGACAGCAAGAAAGCGAGCTTAGTGATACTTGTGGG6521820.6577325059579029No Hit
CTCCAGCTTAACGGTATTTGGAGGTCAGCACGGTGCTCACAGAAGCCAGG5968710.6019507722746096No Hit
CACCAACTTCATCCACGTTCACCTTGCCCCACAGGGCAGTAACGGCAGAC5959320.6010037807552262No Hit
GGTCGAAGTGCGGGAAGTAGGTCTTGGTGGTGGGGAAGGACAGGAACATC5948920.5999549296581457No Hit
GCGGGAAGTAGGTCTTGGTGGTGGGGAAGGACAGGAACATCCTCTCCAGG5792860.584216112137915No Hit
GTCCACGTGCGCCACGGCGTTGGTCAGCGCGTCGGCCACCTTCTTGCCGT5502690.5549521580186961No Hit
CTCGGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGA5273220.5318098636680149No Hit
CTCACCACCAACTTCATCCACGTTCACCTTGCCCCACAGGGCAGTAACGG4825660.48667296010941935No Hit
GGGCATGTCGTCCACGTGCGCCACGGCGTTGGTCAGCGCGTCGGCCACCT4617780.4657080402958506No Hit
GTCCAGGTGAGCCAGGCCATCACTAAAGGCACCGAGCACTTTCTTGCCAT4579340.46183132527933346No Hit
CTTGCCATGAGCCTTCACCTTAGGGTTGCCCATAACAGCATCAGGAGTGG4418300.44559026944530855No Hit
GTCGAAGTGCGGGAAGTAGGTCTTGGTGGTGGGGAAGGACAGGAACATCC4375340.44125770760582944No Hit
GTCCAGGGAGGCGTGCACCGCAGGGGTGAACTCGGCGGGGAGGTGGGCGG4315050.4351773967747728No Hit
CGGTATTTGGAGGTCAGCACGGTGCTCACAGAAGCCAGGAACTTGTCCAG4130250.4165401195881868No Hit
GTCGTCCACGTGCGCCACGGCGTTGGTCAGCGCGTCGGCCACCTTCTTGC3998150.40321769363392257No Hit
CGTCCACGTGCGCCACGGCGTTGGTCAGCGCGTCGGCCACCTTCTTGCCG3954500.3988155445581949No Hit
CTTAGGGTTGCCCATAACAGCATCAGGAGTGGACAGATCCCCAAAGGACT3953620.39872679561921115No Hit
GGCATGTCGTCCACGTGCGCCACGGCGTTGGTCAGCGCGTCGGCCACCTT3950210.3983828934806492No Hit
ATCAGGAGTGGACAGATCCCCAAAGGACTCAAAGAACCTCTGGGTCCAAG3812340.3844785568696394No Hit
GCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGTGCAGGTCGCTC3755350.37873105455977174No Hit
GTCAGCACGGTGCTCACAGAAGCCAGGAACTTGTCCAGGGAGGCGTGCAC3695110.3726557862820664No Hit
CGGGAAGTAGGTCTTGGTGGTGGGGAAGGACAGGAACATCCTCTCCAGGG3580880.3611355688955744No Hit
CCCAGGGCCTCACCACCAACTTCATCCACGTTCACCTTGCCCCACAGGGC3566110.35964599863558594No Hit
GTCACAGTGCAGCTCACTCAGTGTGGCAAAGGTGCCCTTGAGGTTGTCCA3505200.3535031601429725No Hit
CAGCAAGAAAGCGAGCTTAGTGATACTTGTGGGCCAGGGCATTAGCCACA3389890.34187402360409136No Hit
GGGAAGTAGGTCTTGGTGGTGGGGAAGGACAGGAACATCCTCTCCAGGGC3379390.3408150874003081No Hit
CCTTAGGGTTGCCCATAACAGCATCAGGAGTGGACAGATCCCCAAAGGAC3306290.3334428744006358No Hit
CAGGGAGGCGTGCACCGCAGGGGTGAACTCGGCGGGGAGGTGGGCGGCCA3123070.31496494189995244No Hit
ATTTGGAGGTCAGCACGGTGCTCACAGAAGCCAGGAACTTGTCCAGGGAG3083780.3110025034764623No Hit
CCTGCACTGGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAG3047780.30737186506349107No Hit
CTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGTGCAGGTCGCTCA2991450.3016909244578612No Hit
GGGAGGAGGGCCCGTTGGGAGGCCCAGCGGGCAGGAGGAACGGCTACCGA2953210.29783437965474946No Hit
CCAGGAAGGGCCGGTGCAAGGAGGGGAGGAGGGCCCGTTGGGAGGCCCAG2924560.2949449965844265No Hit
GGCCGGTGCAAGGAGGGGAGGAGGGCCCGTTGGGAGGCCCAGCGGGCAGG2883250.2907788390055419No Hit
CCTTGAGGTTGTCCAGGTGAGCCAGGCCATCACTAAAGGCACCGAGCACT2845770.2869989410133707No Hit
GTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCC2793180.2816951833984218No Hit
CCTGGGCAGAGCCGTGGCTCAGGTCGAAGTGCGGGAAGTAGGTCTTGGTG2779640.28032965995087644No Hit
CTCTCCAGGGCCTCCGCACCATACTCGCCAGCGTGCGCGCCGACCTTACC2770540.27941191524093095No Hit
GTCAGCGCGTCGGCCACCTTCTTGCCGTGGCCCTTAACCTGGGCAGAGCC2638240.26606931907326137No Hit
CTGCACTGGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGA2555280.2577027145527031No Hit
CTCTGGGTCCAAGGGTAGACCACCAGCAGCCTGCCCAGGGCCTCACCACC2535280.2556856932121635No Hit
CAGGAAGGGCCGGTGCAAGGAGGGGAGGAGGGCCCGTTGGGAGGCCCAGC2519970.25414166337598043No Hit
GGTATTTGGAGGTCAGCACGGTGCTCACAGAAGCCAGGAACTTGTCCAGG2512380.25337620377724557No Hit
GGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTTGA2508140.2529485952530512No Hit
CTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGTGCAG2465790.24867755256445856No Hit
GCACTTTCTTGCCATGAGCCTTCACCTTAGGGTTGCCCATAACAGCATCA2402320.24227653534025612No Hit
GCCCATAACAGCATCAGGAGTGGACAGATCCCCAAAGGACTCAAAGAACC2393550.24139207148242953No Hit
CGGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGC2389960.24103001615180267No Hit
GTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGT2367180.238732628844928No Hit
CTTCACCTTAGGGTTGCCCATAACAGCATCAGGAGTGGACAGATCCCCAA2348430.23684167133817213No Hit
GCCTGCACTGGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACA2300150.2319725818221095No Hit
CCCCAGTTTAGTAGTTGGACTTAGGGAACAAAGGAACCTTTAATAGAAAT2263260.22825218595948418No Hit
GTAGGTCTTGGTGGTGGGGAAGGACAGGAACATCCTCTCCAGGGCCTCCG2237390.22564316885549618No Hit
GACAGCAAGAAAGCGAGCTTAGTGATACTTGTGGGCCAGGGCATTAGCCA2226030.2244975007340697No Hit
CCAGGAACTTGTCCAGGGAGGCGTGCACCGCAGGGGTGAACTCGGCGGGG2199710.22184310064991955No Hit
CCCATAACAGCATCAGGAGTGGACAGATCCCCAAAGGACTCAAAGAACCT2155510.217385483487327No Hit
GGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTTGAA2146600.21648690048011662No Hit
GGACAGCAAGAAAGCGAGCTTAGTGATACTTGTGGGCCAGGGCATTAGCC2112370.2130347684557831No Hit
CCAGGGAGGCGTGCACCGCAGGGGTGAACTCGGCGGGGAGGTGGGCGGCC2104800.21227132587838884No Hit
CTTGAGGTTGTCCAGGTGAGCCAGGCCATCACTAAAGGCACCGAGCACTT2099240.21171059394571884No Hit
GTTGGGCATGTCGTCCACGTGCGCCACGGCGTTGGTCAGCGCGTCGGCCA2066680.20842688320332034No Hit
GGCAGAATCCAGATGCTCAAGGCCCTTCATAATATCCCCCAGTTTAGTAG2056580.20740828742634784No Hit
GGGGAGGAGGGCCCGTTGGGAGGCCCAGCGGGCAGGAGGAACGGCTACCG2021980.2039188405072143No Hit
CACGGGGGTACGGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAA2012290.20294159366772285No Hit
CAGGAGTGGACAGATCCCCAAAGGACTCAAAGAACCTCTGGGTCCAAGGG2011330.20284477664337697No Hit
GGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGTGC1948990.19655772112491499No Hit
TGGGCATGTCGTCCACGTGCGCCACGGCGTTGGTCAGCGCGTCGGCCACC1942950.19594858068007204No Hit
GTTGGACTTAGGGAACAAAGGAACCTTTAATAGAAATTGGACAGCAAGAA1938830.19553307428392086No Hit
GCTTGTCACAGTGCAGCTCACTCAGTGTGGCAAAGGTGCCCTTGAGGTTG1924360.19407375934404045No Hit
CTCAAAGAACCTCTGGGTCCAAGGGTAGACCACCAGCAGCCTGCCCAGGG1880990.1896998485670803No Hit
CTGCCCAGGGCCTCACCACCAACTTCATCCACGTTCACCTTGCCCCACAG1880200.189620176224129No Hit
GGGAAGGACAGGAACATCCTCTCCAGGGCCTCCGCACCATACTCGCCAGC1854760.18705452507896259No Hit
CTCAGTGTGGCAAAGGTGCCCTTGAGGTTGTCCAGGTGAGCCAGGCCATC1828420.18439810797347195No Hit
GTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTTGAAGTTGA1819380.18348641432754803No Hit
CAGGAACTTGTCCAGGGAGGCGTGCACCGCAGGGGTGAACTCGGCGGGGA1814010.18294484409761314No Hit
GTGGTGGGGAAGGACAGGAACATCCTCTCCAGGGCCTCCGCACCATACTC1786030.18012303124219822No Hit
GCCATCACTAAAGGCACCGAGCACTTTCTTGCCATGAGCCTTCACCTTAG1786020.18012202273152794No Hit
CCACGTGCAGCTTGTCACAGTGCAGCTCACTCAGTGTGGCAAAGGTGCCC1781540.17967020995124708No Hit
GTGAACTCGGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCT1727070.17417685233028746No Hit
CGGCTACCGAGGCTCCAGCTTAACGGTATTTGGAGGTCAGCACGGTGCTC1723720.17383900125574708No Hit
CTCAGGATCCACGTGCAGCTTGTCACAGTGCAGCTCACTCAGTGTGGCAA1722410.1737068863579417No Hit
GTGGGGAAGGACAGGAACATCCTCTCCAGGGCCTCCGCACCATACTCGCC1713180.17277603100928268No Hit
CTTGTCACAGTGCAGCTCACTCAGTGTGGCAAAGGTGCCCTTGAGGTTGT1647280.16612994569220466No Hit
CTTAGTGATACTTGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTC1637980.16519203076885375No Hit
CGGGCATGTCGTCCACGTGCGCCACGGCGTTGGTCAGCGCGTCGGCCACC1632590.16464844351757832No Hit
CTCCAGGGCCTCCGCACCATACTCGCCAGCGTGCGCGCCGACCTTACCCC1615370.1629117881433737No Hit
CACCACTTTCTGATAGGCAGCCTGCACTGGTGGGGTGAATTCTTTGCCAA1557170.15704225604240343No Hit
CATCAGGAGTGGACAGATCCCCAAAGGACTCAAAGAACCTCTGGGTCCAA1528670.15416800063213448No Hit
CGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTTG1515970.15288719208089183No Hit
GTGCAAGGAGGGGAGGAGGGCCCGTTGGGAGGCCCAGCGGGCAGGAGGAA1501390.15141678352363844No Hit
GCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTTGAAGTTGACCGGGTC1467820.14803121320354273No Hit
GGCCTCACCACCAACTTCATCCACGTTCACCTTGCCCCACAGGGCAGTAA1457720.1470126174265702No Hit
CTCACAGAAGCCAGGAACTTGTCCAGGGAGGCGTGCACCGCAGGGGTGAA1444320.14566121312840866No Hit
AGCATCAGGAGTGGACAGATCCCCAAAGGACTCAAAGAACCTCTGGGTCC1442850.14551296205987901No Hit
GTGCGGGAAGTAGGTCTTGGTGGTGGGGAAGGACAGGAACATCCTCTCCA1395390.1407265704187785No Hit
CAGGTCGAAGTGCGGGAAGTAGGTCTTGGTGGTGGGGAAGGACAGGAACA1395000.14068723850263798No Hit
GTGGGAAGTAGGTCTTGGTGGTGGGGAAGGACAGGAACATCCTCTCCAGG1388070.139988340608141No Hit
GGGTGAACTCGGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGG1369630.1381286469321635No Hit
GCAGAATCCAGATGCTCAAGGCCCTTCATAATATCCCCCAGTTTAGTAGT1365500.13771213202534205No Hit
CACCAGCAGCCTGCCCAGGGCCTCACCACCAACTTCATCCACGTTCACCT1358030.13695877455465053No Hit
ACCAACTTCATCCACGTTCACCTTGCCCCACAGGGCAGTAACGGCAGACT1356120.13676614901662898No Hit
CACGTGCGCCACGGCGTTGGTCAGCGCGTCGGCCACCTTCTTGCCGTGGC1332360.1343699276640679No Hit
GGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGC1330070.13413897872057615No Hit
GCCGGTGCAAGGAGGGGAGGAGGGCCCGTTGGGAGGCCCAGCGGGCAGGA1318100.13293179144826317No Hit
CTTAGGGAACAAAGGAACCTTTAATAGAAATTGGACAGCAAGAAAGCGAG1304300.13154004672329084No Hit
ACCGGGTCCACCCGAAGCTTGTGCGCGTGCAGGTCGCTCAGGGCGGACAG1302690.1313776765053774No Hit
CACCAGCAGGCAGTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAA1298460.13095107649185328No Hit
AGCGCGTCGGCCACCTTCTTGCCGTGGCCCTTAACCTGGGCAGAGCCGTG1262280.12730228488681714No Hit
CCACCAACTTCATCCACGTTCACCTTGCCCCACAGGGCAGTAACGGCAGA1261510.12722462956520636No Hit
GTGCGCGTGCAGGTCGCTCAGGGCGGACAGCGCGTTGGGCATGTCGTCCA1258690.12694022955619028No Hit
AGCGCGTTGGGCATGTCGTCCACGTGCGCCACGGCGTTGGTCAGCGCGTC1253670.1264339571997148No Hit
CCTCTGGGTCCAAGGGTAGACCACCAGCAGCCTGCCCAGGGCCTCACCAC1208470.1218754889700953No Hit
GTGCAGCTTGTCACAGTGCAGCTCACTCAGTGTGGCAAAGGTGCCCTTGA1192690.12028405913240953No Hit
TGGGGAAGGACAGGAACATCCTCTCCAGGGCCTCCGCACCATACTCGCCA1175350.11853530163016168No Hit
CTCAGGGCGGACAGCGCGTTGGGCATGTCGTCCACGTGCGCCACGGCGTT1175220.11852219099144819No Hit
GCATGTCGTCCACGTGCGCCACGGCGTTGGTCAGCGCGTCGGCCACCTTC1173590.1183578037521942No Hit
CCACGTTCACCTTGCCCCACAGGGCAGTAACGGCAGACTTCTCCTCAGGA1164370.11742795691420545No Hit
CAGATGCTCAAGGCCCTTCATAATATCCCCCAGTTTAGTAGTTGGACTTA1144410.1154149696163469No Hit
ATCCAGATGCTCAAGGCCCTTCATAATATCCCCCAGTTTAGTAGTTGGAC1134730.11443873128752574No Hit
CCAGGGCCTCACCACCAACTTCATCCACGTTCACCTTGCCCCACAGGGCA1117590.11271014399868329No Hit
CACCTTAGGGTTGCCCATAACAGCATCAGGAGTGGACAGATCCCCAAAGG1117380.11268896527460762No Hit
AGCAAGAAAGCGAGCTTAGTGATACTTGTGGGCCAGGGCATTAGCCACAC1115200.11246910994848881No Hit
GCTTAGTGATACTTGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTT1110090.11195376099598094No Hit
ATCCTCTCCAGGGCCTCCGCACCATACTCGCCAGCGTGCGCGCCGACCTT1106820.11162397800680271No Hit
CCTTCACCTTAGGGTTGCCCATAACAGCATCAGGAGTGGACAGATCCCCA1101220.11105921203145162No Hit
TCCACGTGCGCCACGGCGTTGGTCAGCGCGTCGGCCACCTTCTTGCCGTG1099890.11092508011230572No Hit
CGTTGGGCATGTCGTCCACGTGCGCCACGGCGTTGGTCAGCGCGTCGGCC1088690.10979554816160356No Hit
CCAGCAGGCAGTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGC1086130.10953736943001446No Hit
TGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTTGAAGTTGAC1084310.10935382048802536No Hit
GGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTTGAAGTTGACCGGGT1079790.10889797366506343No Hit
GGAGGAGGGCCCGTTGGGAGGCCCAGCGGGCAGGAGGAACGGCTACCGAG1038220.10470559480875183No Hit
CCACGTGCGCCACGGCGTTGGTCAGCGCGTCGGCCACCTTCTTGCCGTGG1037790.10466222884993022No Hit
CCCGAAGCTTGTGCGCGTGCAGGTCGCTCAGGGCGGACAGCGCGTTGGGC1018970.10276421176848247No Hit
GCCCAGGGCCTCACCACCAACTTCATCCACGTTCACCTTGCCCCACAGGG1016120.10247678622745555No Hit
TGCGGGAAGTAGGTCTTGGTGGTGGGGAAGGACAGGAACATCCTCTCCAG1002900.10114353512135887No Hit
GGAAGTAGGTCTTGGTGGTGGGGAAGGACAGGAACATCCTCTCCAGGGCC1002470.10110016916253728No Hit
AGTAGGTCTTGGTGGTGGGGAAGGACAGGAACATCCTCTCCAGGGCCTCC996420.10049002020702405No Hit
GGGCAGAGCCGTGGCTCAGGTCGAAGTGCGGGAAGTAGGTCTTGGTGGTG992410.10008560742824585No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)