FastQCFastQC Report
Thu 2 May 2024
EGAF00005203681

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005203681
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90062337
Total Bases4.5 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACC45209495.019799786008218No Hit
CAACTGTGTTCACTAGCAACCTCAAACAGACACCATGGTGCACCTGACTC12610071.4001490989513186No Hit
CAACTGTGTTCACTAGCAACCTCAAACAGACACCATGGTGCATCTGACTC11963031.328305526870794No Hit
CTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTC11938481.32557963713511No Hit
CAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAA10813981.2007216734782267No Hit
CTGTGTTCACTAGCAACCTCAAACAGACACCATGGTGCACCTGACTCCTG8271840.9184571792757277No Hit
CACAACTGTGTTCACTAGCAACCTCAAACAGACACCATGGTGCACCTGAC8002540.888555667837045No Hit
CTGTGTTCACTAGCAACCTCAAACAGACACCATGGTGCATCTGACTCCTG7914450.8787746647080676No Hit
CACAACTGTGTTCACTAGCAACCTCAAACAGACACCATGGTGCATCTGAC7454960.8277555577977063No Hit
ACTGTGTTCACTAGCAACCTCAAACAGACACCATGGTGCACCTGACTCCT7403870.8220828202581508No Hit
CCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGAC7362170.8174526939046673No Hit
ACTGTGTTCACTAGCAACCTCAAACAGACACCATGGTGCATCTGACTCCT7145590.793404905759885No Hit
CCAAGACCTACTTCCCGCACTTCGACCTGAGCCACGGCTCTGCCCAGGTT6150640.6829314233762332No Hit
CTCAAGGGCACCTTTGCCACACTGAGTGAGCTGCACTGTGACAAGCTGCA5218440.5794253373638305No Hit
CGCACGTGGACGACATGCCCAACGCGCTGTCCGCCCTGAGCGACCTGCAC5130760.5696898582589524No Hit
AGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGC5073330.5633131638589391No Hit
CTTCAAGCTCCTAAGCCACTGCCTGCTGGTGACCCTGGCCGCCCACCTCC4394940.4879886694479181No Hit
CCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGCCGCCTG4309440.47849524491020035No Hit
GTGTTCACTAGCAACCTCAAACAGACACCATGGTGCACCTGACTCCTGAG4299410.47738157183285174No Hit
GTGTTCACTAGCAACCTCAAACAGACACCATGGTGCATCTGACTCCTGAG4102770.45554780573815223No Hit
CCTCAAGGGCACCTTTGCCACACTGAGTGAGCTGCACTGTGACAAGCTGC3838990.42625920311172916No Hit
CGTGGATGAAGTTGGTGGTGAGGCCCTGGGCAGGCTGCTGGTGGTCTACC3802070.422159820258717No Hit
CCTGGACAACCTCAAGGGCACCTTTGCCACACTGAGTGAGCTGCACTGTG3727730.41390553745013303No Hit
CCAACGTCAAGGCCGCCTGGGGTAAGGTCGGCGCGCACGCTGGCGAGTAT3610120.400846804586028No Hit
CTTTGGCAAAGAATTCACCCCACCAGTGCAGGCTGCCTATCAGAAAGTGG3324700.36915542176081884No Hit
TGTGTTCACTAGCAACCTCAAACAGACACCATGGTGCACCTGACTCCTGA3039950.3375384318530397No Hit
CCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGCCGCCTGG3001610.33328138042875793No Hit
CTCATGGCAAGAAAGTGCTCGGTGCCTTTAGTGATGGCCTGGCTCACCTG2972490.33004806437567796No Hit
CGGCAAGAAGGTGGCCGACGCGCTGACCAACGCCGTGGCGCACGTGGACG2961250.3288000399101347No Hit
GTCAACTTCAAGCTCCTAAGCCACTGCCTGCTGGTGACCCTGGCCGCCCA2935380.3259275850236931No Hit
GTCCACTCCTGATGCTGTTATGGGCAACCCTAAGGTGAAGGCTCATGGCA2891710.3210787212861243No Hit
GACACAACTGTGTTCACTAGCAACCTCAAACAGACACCATGGTGCACCTG2881630.3199594964985197No Hit
TGTGTTCACTAGCAACCTCAAACAGACACCATGGTGCATCTGACTCCTGA2880710.3198573450298098No Hit
CTCAAACAGACACCATGGTGCACCTGACTCCTGAGGAGAAGTCTGCCGTT2874870.31920890527191187No Hit
GTCTGCCGTTACTGCCCTGTGGGGCAAGGTGAACGTGGATGAAGTTGGTG2865740.3181951629791708No Hit
CTTTGCCACACTGAGTGAGCTGCACTGTGACAAGCTGCACGTGGATCCTG2845630.3159622651142175No Hit
ACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCA2822680.31341403010672486No Hit
CTCAAACAGACACCATGGTGCATCTGACTCCTGAGGAGAAGTCTGCCGTT2805790.3115386623822564No Hit
CGGCTCTGCCCAGGTTAAGGGCCACGGCAAGAAGGTGGCCGACGCGCTGA2729220.3030367732962559No Hit
GACACAACTGTGTTCACTAGCAACCTCAAACAGACACCATGGTGCATCTG2694150.29914280372271485No Hit
GGATGTTCCTGTCCTTCCCCACCACCAAGACCTACTTCCCGCACTTCGAC2648730.2940996301261869No Hit
CACTGAGTGAGCTGCACTGTGACAAGCTGCACGTGGATCCTGAGAACTTC2563670.28465506063872187No Hit
CATGCCCAACGCGCTGTCCGCCCTGAGCGACCTGCACGCGCACAAGCTTC2498090.2773734374669847No Hit
CGTGGATCCTGAGAACTTCAGGCTCCTGGGCAACGTGCTGGTCTGTGTGC2482050.2755924488168678No Hit
CAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAA2349790.26090706484776205No Hit
CTAGCAACCTCAAACAGACACCATGGTGCACCTGACTCCTGAGGAGAAGT2200760.24435963725880222No Hit
CTAGCAACCTCAAACAGACACCATGGTGCATCTGACTCCTGAGGAGAAGT2164680.24035352313809044No Hit
GAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGG2148140.23851701738541384No Hit
CAGAGGTTCTTTGAGTCCTTTGGGGATCTGTCCACTCCTGATGCTGTTAT2121170.2355224248733408No Hit
CACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGCCGCCTGGG2095200.23263886656638724No Hit
GACCTACTTCCCGCACTTCGACCTGAGCCACGGCTCTGCCCAGGTTAAGG2073560.23023608636760115No Hit
CTTCAGGCTCCTGGGCAACGTGCTGGTCTGTGTGCTGGCCCATCACTTTG2058340.2285461457656823No Hit
TGAGAACTTCAGGCTCCTGGGCAACGTGCTGGTCTGTGTGCTGGCCCATC2055760.22825967751647397No Hit
GTTCACTAGCAACCTCAAACAGACACCATGGTGCACCTGACTCCTGAGGA2042870.2268284466124835No Hit
GTTATGGGCAACCCTAAGGTGAAGGCTCATGGCAAGAAAGTGCTCGGTGC2040030.22651310946994416No Hit
GGTCAACTTCAAGCTCCTAAGCCACTGCCTGCTGGTGACCCTGGCCGCCC2034960.22595016604998824No Hit
CGTCAAGGCCGCCTGGGGTAAGGTCGGCGCGCACGCTGGCGAGTATGGTG2032900.22572143558744207No Hit
GAGAACTTCAGGCTCCTGGGCAACGTGCTGGTCTGTGTGCTGGCCCATCA2022650.22458333498496713No Hit
CCCTGAGCGACCTGCACGCGCACAAGCTTCGGGTGGACCCGGTCAACTTC2017780.22404259840603513No Hit
CAACGCCGTGGCGCACGTGGACGACATGCCCAACGCGCTGTCCGCCCTGA1998810.22193627953491812No Hit
GGAGAAGTCTGCCGTTACTGCCCTGTGGGGCAAGGTGAACGTGGATGAAG1984920.22039401442580822No Hit
GGTTCTTTGAGTCCTTTGGGGATCTGTCCACTCCTGATGCTGTTATGGGC1940880.2155040680323452No Hit
GCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGAC1931420.21445368445191468No Hit
GTTCACTAGCAACCTCAAACAGACACCATGGTGCATCTGACTCCTGAGGA1922070.21341551463404732No Hit
GTCAAGGCCGCCTGGGGTAAGGTCGGCGCGCACGCTGGCGAGTATGGTGC1890310.20988906827945183No Hit
ACACAACTGTGTTCACTAGCAACCTCAAACAGACACCATGGTGCACCTGA1887420.20956817942665648No Hit
CCCGGTCAACTTCAAGCTCCTAAGCCACTGCCTGCTGGTGACCCTGGCCG1827980.20296830627435306No Hit
CCTCAAACAGACACCATGGTGCATCTGACTCCTGAGGAGAAGTCTGCCGT1800930.19996483102587043No Hit
ACACAACTGTGTTCACTAGCAACCTCAAACAGACACCATGGTGCATCTGA1768440.19635732970153774No Hit
CCCTGGGCAGGCTGCTGGTGGTCTACCCTTGGACCCAGAGGTTCTTTGAG1760380.1954623940082745No Hit
CGGTCAACTTCAAGCTCCTAAGCCACTGCCTGCTGGTGACCCTGGCCGCC1755940.1949694021375439No Hit
CCGGTCAACTTCAAGCTCCTAAGCCACTGCCTGCTGGTGACCCTGGCCGC1747280.194007845921209No Hit
CAACGTCAAGGCCGCCTGGGGTAAGGTCGGCGCGCACGCTGGCGAGTATG1740280.19323060648537246No Hit
CCTCAAACAGACACCATGGTGCACCTGACTCCTGAGGAGAAGTCTGCCGT1738660.19305073107307888No Hit
CTTGGACCCAGAGGTTCTTTGAGTCCTTTGGGGATCTGTCCACTCCTGAT1720970.1910865359845148No Hit
GGCAAGGTGAACGTGGATGAAGTTGGTGGTGAGGCCCTGGGCAGGCTGCT1666290.18501518564858027No Hit
GGCTGCTGGTGGTCTACCCTTGGACCCAGAGGTTCTTTGAGTCCTTTGGG1648630.1830543215861698No Hit
CGCTGACCAACGCCGTGGCGCACGTGGACGACATGCCCAACGCGCTGTCC1639150.1820017173216369No Hit
CCTGCACGCGCACAAGCTTCGGGTGGACCCGGTCAACTTCAAGCTCCTAA1564450.17370746219920985No Hit
CCTGACTCCTGAGGAGAAGTCTGCCGTTACTGCCCTGTGGGGCAAGGTGA1537980.17076838678969655No Hit
CTAAGGTGAAGGCTCATGGCAAGAAAGTGCTCGGTGCCTTTAGTGATGGC1517130.1684533236129549No Hit
CCTGGCTCACCTGGACAACCTCAAGGGCACCTTTGCCACACTGAGTGAGC1490510.16549759307267364No Hit
CAAGCTTCGGGTGGACCCGGTCAACTTCAAGCTCCTAAGCCACTGCCTGC1484880.16487247049785084No Hit
CTTCGGGTGGACCCGGTCAACTTCAAGCTCCTAAGCCACTGCCTGCTGGT1483450.16471369158452995No Hit
CTGAGAACTTCAGGCTCCTGGGCAACGTGCTGGTCTGTGTGCTGGCCCAT1477070.1640052933558675No Hit
CCTGGCTTCTGTGAGCACCGTGCTGACCTCCAAATACCGTTAAGCTGGAG1474060.1636710803984578No Hit
CCTACTTCCCGCACTTCGACCTGAGCCACGGCTCTGCCCAGGTTAAGGGC1462250.16235976643599645No Hit
CCCTGGCCGCCCACCTCCCCGCCGAGTTCACCCCTGCGGTGCACGCCTCC1451190.1611317281273747No Hit
CGCCTCCCTGGACAAGTTCCTGGCTTCTGTGAGCACCGTGCTGACCTCCA1444460.16038446792692043No Hit
CACCTCCCCGCCGAGTTCACCCCTGCGGTGCACGCCTCCCTGGACAAGTT1439810.15986815887311473No Hit
AGAGGATGTTCCTGTCCTTCCCCACCACCAAGACCTACTTCCCGCACTTC1426540.1583947349711789No Hit
CCACCACCAAGACCTACTTCCCGCACTTCGACCTGAGCCACGGCTCTGCC1421970.15788730865378275No Hit
CTTCGACCTGAGCCACGGCTCTGCCCAGGTTAAGGGCCACGGCAAGAAGG1411420.156715897789772No Hit
CAGAAAGTGGTGGCTGGTGTGGCTAATGCCCTGGCCCACAAGTATCACTA1411370.156710346079516No Hit
GTGCTGGTCTGTGTGCTGGCCCATCACTTTGGCAAAGAATTCACCCCACC1378720.15308507928236417No Hit
GCCACGGCAAGAAGGTGGCCGACGCGCTGACCAACGCCGTGGCGCACGTG1365950.15166717248298808No Hit
GTTGGTGGTGAGGCCCTGGGCAGGCTGCTGGTGGTCTACCCTTGGACCCA1354790.1504280307538544No Hit
GTTCTTTGAGTCCTTTGGGGATCTGTCCACTCCTGATGCTGTTATGGGCA1335340.14826841546428002No Hit
TGCTGTTATGGGCAACCCTAAGGTGAAGGCTCATGGCAAGAAAGTGCTCG1330530.1477343409376552No Hit
CCTGGCCGCCCACCTCCCCGCCGAGTTCACCCCTGCGGTGCACGCCTCCC1320820.14665619880594483No Hit
CCCACCTCCCCGCCGAGTTCACCCCTGCGGTGCACGCCTCCCTGGACAAG1318300.14637639260904367No Hit
CCATGGTGCACCTGACTCCTGAGGAGAAGTCTGCCGTTACTGCCCTGTGG1311500.145621360014231No Hit
CTGTGACAAGCTGCACGTGGATCCTGAGAACTTCAGGCTCCTGGGCAACG1305380.14494183067889965No Hit
CTACCCTTGGACCCAGAGGTTCTTTGAGTCCTTTGGGGATCTGTCCACTC1297470.14406355011640437No Hit
GCAACCTCAAACAGACACCATGGTGCACCTGACTCCTGAGGAGAAGTCTG1280540.14218374102373116No Hit
CCTGGAGAGGATGTTCCTGTCCTTCCCCACCACCAAGACCTACTTCCCGC1274680.14153308058173086No Hit
GCAACCTCAAACAGACACCATGGTGCATCTGACTCCTGAGGAGAAGTCTG1266490.1406237104418021No Hit
CCCAGAGGTTCTTTGAGTCCTTTGGGGATCTGTCCACTCCTGATGCTGTT1264340.14038498690079518No Hit
CGTTACTGCCCTGTGGGGCAAGGTGAACGTGGATGAAGTTGGTGGTGAGG1255190.1393690239239517No Hit
CGAGTATGGTGCGGAGGCCCTGGAGAGGATGTTCCTGTCCTTCCCCACCA1246310.13838304018249048No Hit
CCCCTGCGGTGCACGCCTCCCTGGACAAGTTCCTGGCTTCTGTGAGCACC1244460.1381776269030194No Hit
GTCGGCGCGCACGCTGGCGAGTATGGTGCGGAGGCCCTGGAGAGGATGTT1243700.13809324090712857No Hit
CACGGCTCTGCCCAGGTTAAGGGCCACGGCAAGAAGGTGGCCGACGCGCT1235440.13717609837284148No Hit
CCTAAGGTGAAGGCTCATGGCAAGAAAGTGCTCGGTGCCTTTAGTGATGG1215380.13494875221814417No Hit
CATCACTTTGGCAAAGAATTCACCCCACCAGTGCAGGCTGCCTATCAGAA1205830.13388837555925293No Hit
CTGGACAACCTCAAGGGCACCTTTGCCACACTGAGTGAGCTGCACTGTGA1196820.13288795737112616No Hit
AACGCCGTGGCGCACGTGGACGACATGCCCAACGCGCTGTCCGCCCTGAG1195260.13271474401113975No Hit
AGCAACCTCAAACAGACACCATGGTGCACCTGACTCCTGAGGAGAAGTCT1180850.13111474111536767No Hit
CAAGACCAACGTCAAGGCCGCCTGGGGTAAGGTCGGCGCGCACGCTGGCG1175480.13051848743387595No Hit
CTTTAGTGATGGCCTGGCTCACCTGGACAACCTCAAGGGCACCTTTGCCA1168960.12979454441649677No Hit
GATCTGTCCACTCCTGATGCTGTTATGGGCAACCCTAAGGTGAAGGCTCA1162970.12912944952783093No Hit
GTGAACGTGGATGAAGTTGGTGGTGAGGCCCTGGGCAGGCTGCTGGTGGT1158690.12865422312991945No Hit
AGCAACCTCAAACAGACACCATGGTGCATCTGACTCCTGAGGAGAAGTCT1158060.12858427158069416No Hit
CACCACCAAGACCTACTTCCCGCACTTCGACCTGAGCCACGGCTCTGCCC1133510.1258583818450103No Hit
ATTCACCCCACCAGTGCAGGCTGCCTATCAGAAAGTGGTGGCTGGTGTGG1128800.12533541073889742No Hit
TGGCAAGAAAGTGCTCGGTGCCTTTAGTGATGGCCTGGCTCACCTGGACA1117070.12403297951284564No Hit
GGACAACCTCAAGGGCACCTTTGCCACACTGAGTGAGCTGCACTGTGACA1101690.12232527343810766No Hit
CCTGTCCTTCCCCACCACCAAGACCTACTTCCCGCACTTCGACCTGAGCC1083440.12029889919467668No Hit
CGCCGTGGCGCACGTGGACGACATGCCCAACGCGCTGTCCGCCCTGAGCG1076200.11949501154961145No Hit
CGCGCACAAGCTTCGGGTGGACCCGGTCAACTTCAAGCTCCTAAGCCACT1073480.11919299851168641No Hit
GGCCACGGCAAGAAGGTGGCCGACGCGCTGACCAACGCCGTGGCGCACGT1049290.11650708108984559No Hit
CGACATGCCCAACGCGCTGTCCGCCCTGAGCGACCTGCACGCGCACAAGC1032490.11464170644383789No Hit
CCTTGGACCCAGAGGTTCTTTGAGTCCTTTGGGGATCTGTCCACTCCTGA1032320.11462283062896757No Hit
TGTTCCTGTCCTTCCCCACCACCAAGACCTACTTCCCGCACTTCGACCTG1028160.11416092833567044No Hit
GCTGCACTGTGACAAGCTGCACGTGGATCCTGAGAACTTCAGGCTCCTGG1026670.11399548737004238No Hit
CCTGGGCAGGCTGCTGGTGGTCTACCCTTGGACCCAGAGGTTCTTTGAGT1023390.11363129517725039No Hit
AACTGTGTTCACTAGCAACCTCAAACAGACACCATGGTGCACCTGACTCC1018410.11307834483575527No Hit
GTTCCTGTCCTTCCCCACCACCAAGACCTACTTCCCGCACTTCGACCTGA999990.11103309477745398No Hit
CGTGCTGGTCTGTGTGCTGGCCCATCACTTTGGCAAAGAATTCACCCCAC997930.1108043643149078No Hit
GCCGTTACTGCCCTGTGGGGCAAGGTGAACGTGGATGAAGTTGGTGGTGA996840.11068333703132753No Hit
CACAAGTATCACTAAGCTCGCTTTCTTGCTGTCCAATTTCTATTAAAGGT993220.11028139320879492No Hit
CGCCGAGTTCACCCCTGCGGTGCACGCCTCCCTGGACAAGTTCCTGGCTT983300.10917993389400944No Hit
CGCGCTGTCCGCCCTGAGCGACCTGCACGCGCACAAGCTTCGGGTGGACC981520.10898229300889671No Hit
AACTGTGTTCACTAGCAACCTCAAACAGACACCATGGTGCATCTGACTCC979750.1087857624658352No Hit
GTGCGGAGGCCCTGGAGAGGATGTTCCTGTCCTTCCCCACCACCAAGACC968540.10754106902644553No Hit
GGTGAACGTGGATGAAGTTGGTGGTGAGGCCCTGGGCAGGCTGCTGGTGG956370.10618978275014115No Hit
GGATGAAGTTGGTGGTGAGGCCCTGGGCAGGCTGCTGGTGGTCTACCCTT950640.10555355675480639No Hit
GTTCACCCCTGCGGTGCACGCCTCCCTGGACAAGTTCCTGGCTTCTGTGA944940.1049206617856252No Hit
GCCCTGGGCAGGCTGCTGGTGGTCTACCCTTGGACCCAGAGGTTCTTTGA941620.10455202822462846No Hit
CAAACAGACACCATGGTGCACCTGACTCCTGAGGAGAAGTCTGCCGTTAC938250.10417784295337572No Hit
CTTTGAGTCCTTTGGGGATCTGTCCACTCCTGATGCTGTTATGGGCAACC937940.10414342234978868No Hit
GCTCATGGCAAGAAAGTGCTCGGTGCCTTTAGTGATGGCCTGGCTCACCT929910.10325181768267905No Hit
CAAACAGACACCATGGTGCATCTGACTCCTGAGGAGAAGTCTGCCGTTAC918210.10195271748278084No Hit
CTGGAGAGGATGTTCCTGTCCTTCCCCACCACCAAGACCTACTTCCCGCA917770.10190386243252826No Hit
GTTCCTGGCTTCTGTGAGCACCGTGCTGACCTCCAAATACCGTTAAGCTG907120.10072134814800554No Hit
GTGCAGGCTGCCTATCAGAAAGTGGTGGCTGGTGTGGCTAATGCCCTGGC906850.10069136891262326No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)