FastQCFastQC Report
Sun 5 Jun 2022
EGAF00005203681

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005203681
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90062337
Sequences flagged as poor quality0
Sequence length51
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCA45190755.017719005004278No Hit
CAACTGTGTTCACTAGCAACCTCAAACAGACACCATGGTGCACCTGACTCC12601451.3991919841031886No Hit
CAACTGTGTTCACTAGCAACCTCAAACAGACACCATGGTGCATCTGACTCC11954461.3273539637329197No Hit
CTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA11933791.3250588867130997No Hit
CAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAAC10809411.2002142471608304No Hit
CTGTGTTCACTAGCAACCTCAAACAGACACCATGGTGCACCTGACTCCTGA8264660.9176599536829696No Hit
CACAACTGTGTTCACTAGCAACCTCAAACAGACACCATGGTGCACCTGACT7993670.887570794437635No Hit
CTGTGTTCACTAGCAACCTCAAACAGACACCATGGTGCATCTGACTCCTGA7906700.8779141496183915No Hit
CACAACTGTGTTCACTAGCAACCTCAAACAGACACCATGGTGCATCTGACT7447500.8269272426275148No Hit
ACTGTGTTCACTAGCAACCTCAAACAGACACCATGGTGCACCTGACTCCTG7397690.8213966288705122No Hit
CCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACC7357840.8169719157965No Hit
ACTGTGTTCACTAGCAACCTCAAACAGACACCATGGTGCATCTGACTCCTG7139320.7927087212937856No Hit
CCAAGACCTACTTCCCGCACTTCGACCTGAGCCACGGCTCTGCCCAGGTTA6147670.6826016517870284No Hit
CTCAAGGGCACCTTTGCCACACTGAGTGAGCTGCACTGTGACAAGCTGCAC5216390.5791977172433356No Hit
CGCACGTGGACGACATGCCCAACGCGCTGTCCGCCCTGAGCGACCTGCACG5127250.5693001281989829No Hit
AGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGCC5071040.5630588955292155No Hit
CTTCAAGCTCCTAAGCCACTGCCTGCTGGTGACCCTGGCCGCCCACCTCCC4393380.4878154560879316No Hit
CCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGCCGCCTGG4307400.4782687351317566No Hit
GTGTTCACTAGCAACCTCAAACAGACACCATGGTGCACCTGACTCCTGAGG4296650.4770751174267219No Hit
GTGTTCACTAGCAACCTCAAACAGACACCATGGTGCATCTGACTCCTGAGG4100020.4552424616740736No Hit
CCTCAAGGGCACCTTTGCCACACTGAGTGAGCTGCACTGTGACAAGCTGCA3837330.4260748863312308No Hit
CGTGGATGAAGTTGGTGGTGAGGCCCTGGGCAGGCTGCTGGTGGTCTACCC3799880.42191665534950534No Hit
CCTGGACAACCTCAAGGGCACCTTTGCCACACTGAGTGAGCTGCACTGTGA3725310.4136368346737438No Hit
CCAACGTCAAGGCCGCCTGGGGTAAGGTCGGCGCGCACGCTGGCGAGTATG3608270.40064139130655696No Hit
CTTTGGCAAAGAATTCACCCCACCAGTGCAGGCTGCCTATCAGAAAGTGGT3322120.36886895351161053No Hit
TGTGTTCACTAGCAACCTCAAACAGACACCATGGTGCACCTGACTCCTGAG3038950.33742739764792024No Hit
CCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGCCGCCTGGG3000030.3331059463846691No Hit
CTCATGGCAAGAAAGTGCTCGGTGCCTTTAGTGATGGCCTGGCTCACCTGG2970600.3298382097280021No Hit
CGGCAAGAAGGTGGCCGACGCGCTGACCAACGCCGTGGCGCACGTGGACGA2960250.3286890057050152No Hit
GTCAACTTCAAGCTCCTAAGCCACTGCCTGCTGGTGACCCTGGCCGCCCAC2934200.3257965646616521No Hit
GTCCACTCCTGATGCTGTTATGGGCAACCCTAAGGTGAAGGCTCATGGCAA2889090.3207878116687112No Hit
GACACAACTGTGTTCACTAGCAACCTCAAACAGACACCATGGTGCACCTGA2881060.31989620700160154No Hit
TGTGTTCACTAGCAACCTCAAACAGACACCATGGTGCATCTGACTCCTGAG2879920.3197696280077653No Hit
CTCAAACAGACACCATGGTGCACCTGACTCCTGAGGAGAAGTCTGCCGTTA2874170.3191311813283282No Hit
GTCTGCCGTTACTGCCCTGTGGGGCAAGGTGAACGTGGATGAAGTTGGTGG2860530.3176166747704981No Hit
CTTTGCCACACTGAGTGAGCTGCACTGTGACAAGCTGCACGTGGATCCTGA2843640.3157413070460297No Hit
ACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAA2821650.3132996648754518No Hit
CTCAAACAGACACCATGGTGCATCTGACTCCTGAGGAGAAGTCTGCCGTTA2805290.31148314527969667No Hit
CGGCTCTGCCCAGGTTAAGGGCCACGGCAAGAAGGTGGCCGACGCGCTGAC2728100.30291241498652205No Hit
GACACAACTGTGTTCACTAGCAACCTCAAACAGACACCATGGTGCATCTGA2693630.2990850659360527No Hit
GGATGTTCCTGTCCTTCCCCACCACCAAGACCTACTTCCCGCACTTCGACC2646400.2938409204282585No Hit
CACTGAGTGAGCTGCACTGTGACAAGCTGCACGTGGATCCTGAGAACTTCA2561540.28441855778181724No Hit
CATGCCCAACGCGCTGTCCGCCCTGAGCGACCTGCACGCGCACAAGCTTCG2497220.27727683770853073No Hit
CGTGGATCCTGAGAACTTCAGGCTCCTGGGCAACGTGCTGGTCTGTGTGCT2480890.2754636491389292No Hit
CAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAG2348290.26074051354008276No Hit
CTAGCAACCTCAAACAGACACCATGGTGCACCTGACTCCTGAGGAGAAGTC2199800.24425304442188747No Hit
CTAGCAACCTCAAACAGACACCATGGTGCATCTGACTCCTGAGGAGAAGTC2163810.2402569233796365No Hit
GAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGC2146580.23834380402542743No Hit
CAGAGGTTCTTTGAGTCCTTTGGGGATCTGTCCACTCCTGATGCTGTTATG2119950.23538696314309498No Hit
CACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGCCGCCTGGGG2093100.23240569473563627No Hit
GACCTACTTCCCGCACTTCGACCTGAGCCACGGCTCTGCCCAGGTTAAGGG2072720.23014281763530076No Hit
CTTCAGGCTCCTGGGCAACGTGCTGGTCTGTGTGCTGGCCCATCACTTTGG2057630.22846731148004742No Hit
TGAGAACTTCAGGCTCCTGGGCAACGTGCTGGTCTGTGTGCTGGCCCATCA2055270.2282052707559654No Hit
GTTCACTAGCAACCTCAAACAGACACCATGGTGCACCTGACTCCTGAGGAG2042000.22673184685402956No Hit
GTTATGGGCAACCCTAAGGTGAAGGCTCATGGCAAGAAAGTGCTCGGTGCC2038570.22635099953046967No Hit
GGTCAACTTCAAGCTCCTAAGCCACTGCCTGCTGGTGACCCTGGCCGCCCA2034000.2258435732130735No Hit
CGTCAAGGCCGCCTGGGGTAAGGTCGGCGCGCACGCTGGCGAGTATGGTGC2031830.22560262898796418No Hit
GAGAACTTCAGGCTCCTGGGCAACGTGCTGGTCTGTGTGCTGGCCCATCAC2021640.22447119043779643No Hit
CCCTGAGCGACCTGCACGCGCACAAGCTTCGGGTGGACCCGGTCAACTTCA2016980.22395377104193953No Hit
CAACGCCGTGGCGCACGTGGACGACATGCCCAACGCGCTGTCCGCCCTGAG1998060.2218530038810785No Hit
GGAGAAGTCTGCCGTTACTGCCCTGTGGGGCAAGGTGAACGTGGATGAAGT1982370.22011087720275346No Hit
GGTTCTTTGAGTCCTTTGGGGATCTGTCCACTCCTGATGCTGTTATGGGCA1940260.21543522682517113No Hit
GCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACC1930370.2143370985365392No Hit
GTTCACTAGCAACCTCAAACAGACACCATGGTGCATCTGACTCCTGAGGAG1921260.21332557692790052No Hit
GTCAAGGCCGCCTGGGGTAAGGTCGGCGCGCACGCTGGCGAGTATGGTGCG1889510.2098002409153562No Hit
ACACAACTGTGTTCACTAGCAACCTCAAACAGACACCATGGTGCACCTGAC1886760.20949489685127756No Hit
CCCGGTCAACTTCAAGCTCCTAAGCCACTGCCTGCTGGTGACCCTGGCCGC1827350.20289835472512777No Hit
CCTCAAACAGACACCATGGTGCATCTGACTCCTGAGGAGAAGTCTGCCGTT1798910.19974054193152907No Hit
ACACAACTGTGTTCACTAGCAACCTCAAACAGACACCATGGTGCATCTGAC1767840.196290709178466No Hit
CCCTGGGCAGGCTGCTGGTGGTCTACCCTTGGACCCAGAGGTTCTTTGAGT1759080.19531804954161916No Hit
CGGTCAACTTCAAGCTCCTAAGCCACTGCCTGCTGGTGACCCTGGCCGCCC1754770.19483949211755408No Hit
CCGGTCAACTTCAAGCTCCTAAGCCACTGCCTGCTGGTGACCCTGGCCGCC1746830.19395788052890522No Hit
CAACGTCAAGGCCGCCTGGGGTAAGGTCGGCGCGCACGCTGGCGAGTATGG1739180.193108468859741No Hit
CCTCAAACAGACACCATGGTGCACCTGACTCCTGAGGAGAAGTCTGCCGTT1736770.192840876425403No Hit
CTTGGACCCAGAGGTTCTTTGAGTCCTTTGGGGATCTGTCCACTCCTGATG1719520.19092553638709153No Hit
GGCAAGGTGAACGTGGATGAAGTTGGTGGTGAGGCCCTGGGCAGGCTGCTG1665200.184894158365No Hit
GGCTGCTGGTGGTCTACCCTTGGACCCAGAGGTTCTTTGAGTCCTTTGGGG1647800.18296216319592062No Hit
CGCTGACCAACGCCGTGGCGCACGTGGACGACATGCCCAACGCGCTGTCCG1638490.18192843474625803No Hit
CCTGCACGCGCACAAGCTTCGGGTGGACCCGGTCAACTTCAAGCTCCTAAG1563740.17362862791357503No Hit
CCTGACTCCTGAGGAGAAGTCTGCCGTTACTGCCCTGTGGGGCAAGGTGAA1534440.1703753257035735No Hit
CTAAGGTGAAGGCTCATGGCAAGAAAGTGCTCGGTGCCTTTAGTGATGGCC1516160.16834562043398896No Hit
CCTGGCTCACCTGGACAACCTCAAGGGCACCTTTGCCACACTGAGTGAGCT1489430.16537767613114457No Hit
CAAGCTTCGGGTGGACCCGGTCAACTTCAAGCTCCTAAGCCACTGCCTGCT1483650.16473589842555383No Hit
CTTCGGGTGGACCCGGTCAACTTCAAGCTCCTAAGCCACTGCCTGCTGGTG1482750.1646359676409463No Hit
CTGAGAACTTCAGGCTCCTGGGCAACGTGCTGGTCTGTGTGCTGGCCCATC1476500.1639420038589494No Hit
CCTGGCTTCTGTGAGCACCGTGCTGACCTCCAAATACCGTTAAGCTGGAGC1472660.1635156325112905No Hit
CCTACTTCCCGCACTTCGACCTGAGCCACGGCTCTGCCCAGGTTAAGGGCC1461710.1622998079652319No Hit
CCCTGGCCGCCCACCTCCCCGCCGAGTTCACCCCTGCGGTGCACGCCTCCC1450260.16102846631661358No Hit
CGCCTCCCTGGACAAGTTCCTGGCTTCTGTGAGCACCGTGCTGACCTCCAA1443610.16029008885256885No Hit
CACCTCCCCGCCGAGTTCACCCCTGCGGTGCACGCCTCCCTGGACAAGTTC1439390.15982152450696455No Hit
AGAGGATGTTCCTGTCCTTCCCCACCACCAAGACCTACTTCCCGCACTTCG1425360.15826371460913788No Hit
CCACCACCAAGACCTACTTCCCGCACTTCGACCTGAGCCACGGCTCTGCCC1421290.1578118053943015No Hit
CTTCGACCTGAGCCACGGCTCTGCCCAGGTTAAGGGCCACGGCAAGAAGGT1410410.15660375324260128No Hit
CAGAAAGTGGTGGCTGGTGTGGCTAATGCCCTGGCCCACAAGTATCACTAA1410140.156573774007219No Hit
GTGCTGGTCTGTGTGCTGGCCCATCACTTTGGCAAAGAATTCACCCCACCA1378170.15302401046954844No Hit
GCCACGGCAAGAAGGTGGCCGACGCGCTGACCAACGCCGTGGCGCACGTGG1365330.15159833127581399No Hit
GTTGGTGGTGAGGCCCTGGGCAGGCTGCTGGTGGTCTACCCTTGGACCCAG1354120.15035363783642433No Hit
GTTCTTTGAGTCCTTTGGGGATCTGTCCACTCCTGATGCTGTTATGGGCAA1334500.14817514673197965No Hit
TGCTGTTATGGGCAACCCTAAGGTGAAGGCTCATGGCAAGAAAGTGCTCGG1329800.147653285967918No Hit
CCTGGCCGCCCACCTCCCCGCCGAGTTCACCCCTGCGGTGCACGCCTCCCT1319820.1465451646008253No Hit
CCCACCTCCCCGCCGAGTTCACCCCTGCGGTGCACGCCTCCCTGGACAAGT1317520.14628978592905045No Hit
CCATGGTGCACCTGACTCCTGAGGAGAAGTCTGCCGTTACTGCCCTGTGGG1311150.14558249804243922No Hit
CTGTGACAAGCTGCACGTGGATCCTGAGAACTTCAGGCTCCTGGGCAACGT1304610.14485633434095763No Hit
CTACCCTTGGACCCAGAGGTTCTTTGAGTCCTTTGGGGATCTGTCCACTCC1296960.14400692267179344No Hit
GCAACCTCAAACAGACACCATGGTGCACCTGACTCCTGAGGAGAAGTCTGC1279780.14209935502784032No Hit
CCTGGAGAGGATGTTCCTGTCCTTCCCCACCACCAAGACCTACTTCCCGCA1274240.14148422553147827No Hit
GCAACCTCAAACAGACACCATGGTGCATCTGACTCCTGAGGAGAAGTCTGC1265650.1405304417095017No Hit
CCCAGAGGTTCTTTGAGTCCTTTGGGGATCTGTCCACTCCTGATGCTGTTA1263790.14032391808797945No Hit
CGTTACTGCCCTGTGGGGCAAGGTGAACGTGGATGAAGTTGGTGGTGAGGC1254510.13929352066447043No Hit
CGAGTATGGTGCGGAGGCCCTGGAGAGGATGTTCCTGTCCTTCCCCACCAC1245830.13832974376403312No Hit
CCCCTGCGGTGCACGCCTCCCTGGACAAGTTCCTGGCTTCTGTGAGCACCG1243700.13809324090712857No Hit
GTCGGCGCGCACGCTGGCGAGTATGGTGCGGAGGCCCTGGAGAGGATGTTC1242990.13801440662149375No Hit
CACGGCTCTGCCCAGGTTAAGGGCCACGGCAAGAAGGTGGCCGACGCGCTG1234220.13704063664259566No Hit
CCTAAGGTGAAGGCTCATGGCAAGAAAGTGCTCGGTGCCTTTAGTGATGGC1214990.1349054488781476No Hit
CATCACTTTGGCAAAGAATTCACCCCACCAGTGCAGGCTGCCTATCAGAAA1204970.13379288614285015No Hit
CTGGACAACCTCAAGGGCACCTTTGCCACACTGAGTGAGCTGCACTGTGAC1196190.13281800582190087No Hit
AACGCCGTGGCGCACGTGGACGACATGCCCAACGCGCTGTCCGCCCTGAGC1194720.13265478554037521No Hit
AGCAACCTCAAACAGACACCATGGTGCACCTGACTCCTGAGGAGAAGTCTG1180300.13105367230255197No Hit
CAAGACCAACGTCAAGGCCGCCTGGGGTAAGGTCGGCGCGCACGCTGGCGA1175060.13047185306772574No Hit
CTTTAGTGATGGCCTGGCTCACCTGGACAACCTCAAGGGCACCTTTGCCAC1168300.12972126184111787No Hit
GATCTGTCCACTCCTGATGCTGTTATGGGCAACCCTAAGGTGAAGGCTCAT1162230.1290472842160425No Hit
GTGAACGTGGATGAAGTTGGTGGTGAGGCCCTGGGCAGGCTGCTGGTGGTC1158190.1285987060273597No Hit
AGCAACCTCAAACAGACACCATGGTGCATCTGACTCCTGAGGAGAAGTCTG1157390.12850987866326408No Hit
CACCACCAAGACCTACTTCCCGCACTTCGACCTGAGCCACGGCTCTGCCCA1132150.12570737532604778No Hit
ATTCACCCCACCAGTGCAGGCTGCCTATCAGAAAGTGGTGGCTGGTGTGGC1128330.12528322466249128No Hit
TGGCAAGAAAGTGCTCGGTGCCTTTAGTGATGGCCTGGCTCACCTGGACAA1115950.1239086212031118No Hit
GGACAACCTCAAGGGCACCTTTGCCACACTGAGTGAGCTGCACTGTGACAA1101010.1222497701786264No Hit
CCTGTCCTTCCCCACCACCAAGACCTACTTCCCGCACTTCGACCTGAGCCA1082990.12024893380237289No Hit
CGCCGTGGCGCACGTGGACGACATGCCCAACGCGCTGTCCGCCCTGAGCGA1075880.11945948060397321No Hit
CGCGCACAAGCTTCGGGTGGACCCGGTCAACTTCAAGCTCCTAAGCCACTG1072850.11912304696246112No Hit
GGCCACGGCAAGAAGGTGGCCGACGCGCTGACCAACGCCGTGGCGCACGTG1048760.11644823296113226No Hit
CGACATGCCCAACGCGCTGTCCGCCCTGAGCGACCTGCACGCGCACAAGCT1031730.11455732044794707No Hit
CCTTGGACCCAGAGGTTCTTTGAGTCCTTTGGGGATCTGTCCACTCCTGAT1031360.11451623779205286No Hit
TGTTCCTGTCCTTCCCCACCACCAAGACCTACTTCCCGCACTTCGACCTGA1027670.11410652157516188No Hit
GCTGCACTGTGACAAGCTGCACGTGGATCCTGAGAACTTCAGGCTCCTGGG1025970.11391776342645872No Hit
CCTGGGCAGGCTGCTGGTGGTCTACCCTTGGACCCAGAGGTTCTTTGAGTC1022980.1135857711531514No Hit
AACTGTGTTCACTAGCAACCTCAAACAGACACCATGGTGCACCTGACTCCT1016470.11286293847782342No Hit
GTTCCTGTCCTTCCCCACCACCAAGACCTACTTCCCGCACTTCGACCTGAG999630.11099312246361095No Hit
CGTGCTGGTCTGTGTGCTGGCCCATCACTTTGGCAAAGAATTCACCCCACC997610.11076883336926956No Hit
GCCGTTACTGCCCTGTGGGGCAAGGTGAACGTGGATGAAGTTGGTGGTGAG996420.11063670266517735No Hit
CACAAGTATCACTAAGCTCGCTTTCTTGCTGTCCAATTTCTATTAAAGGTT992310.11018035208213617No Hit
CGCCGAGTTCACCCCTGCGGTGCACGCCTCCCTGGACAAGTTCCTGGCTTC982950.10914107192221761No Hit
CGCGCTGTCCGCCCTGAGCGACCTGCACGCGCACAAGCTTCGGGTGGACCC981010.10892566556428578No Hit
AACTGTGTTCACTAGCAACCTCAAACAGACACCATGGTGCATCTGACTCCT977980.10858923192277367No Hit
GTGCGGAGGCCCTGGAGAGGATGTTCCTGTCCTTCCCCACCACCAAGACCT967690.10744668995209396No Hit
GGTGAACGTGGATGAAGTTGGTGGTGAGGCCCTGGGCAGGCTGCTGGTGGT955540.10609762435989196No Hit
GGATGAAGTTGGTGGTGAGGCCCTGGGCAGGCTGCTGGTGGTCTACCCTTG949970.10547916383737634No Hit
GTTCACCCCTGCGGTGCACGCCTCCCTGGACAAGTTCCTGGCTTCTGTGAG944560.10487846878767981No Hit
GCCCTGGGCAGGCTGCTGGTGGTCTACCCTTGGACCCAGAGGTTCTTTGAG941120.1044965111220687No Hit
CAAACAGACACCATGGTGCACCTGACTCCTGAGGAGAAGTCTGCCGTTACT937590.10410456037799685No Hit
CTTTGAGTCCTTTGGGGATCTGTCCACTCCTGATGCTGTTATGGGCAACCC937450.10408901558928013No Hit
GCTCATGGCAAGAAAGTGCTCGGTGCCTTTAGTGATGGCCTGGCTCACCTG929370.10319185921191451No Hit
CAAACAGACACCATGGTGCATCTGACTCCTGAGGAGAAGTCTGCCGTTACT917370.10185944875048046No Hit
CTGGAGAGGATGTTCCTGTCCTTCCCCACCACCAAGACCTACTTCCCGCAC916940.10181170404227907No Hit
GTTCCTGGCTTCTGTGAGCACCGTGCTGACCTCCAAATACCGTTAAGCTGG906640.10066805172954817No Hit
GTGCAGGCTGCCTATCAGAAAGTGGTGGCTGGTGTGGCTAATGCCCTGGCC906590.1006625000192922No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)