FastQCFastQC Report
Fri 10 May 2024
EGAF00005203682

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005203682
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28282472
Total Bases1.4 Gbp
Sequences flagged as poor quality0
Sequence length35-51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCGTCTT1844530.6521813227641488No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA1662030.5876537241864855No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT1617250.5718205961628814No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT1436390.5078728620327105No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT1100800.3892163315851599No Hit
CTCCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTAC1097320.3879858875136516No Hit
GTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGG1092810.38639126028304743No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG1007690.35629488115466No Hit
CTCGTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCAACTAAGAAC928080.3281467051394942No Hit
CTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGA916760.32414422614826593No Hit
CTCCGACTTTCGTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCG878550.3106340916734577No Hit
CCCGTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGG864550.30568402931681504No Hit
CTCGTTCATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT852960.3015860848372801No Hit
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC851370.3010238991839186No Hit
CTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATT830790.29374730751965383No Hit
CTGATAAATGCACGCATCCCCCCCGCGAAGGGGGTCAGCGCCCGTCGGCA817770.28914374952797617No Hit
CTAAGAAGTTGGGGGACGCCGACCGCTCGGGGGTCGCGTAACTAGTTAGC788870.27892540652033526No Hit
CTTTAAGTTTCAGCTTTGCAACCATACTCCCCCCGGAACCCAAAGACTTT784650.2774333162956901No Hit
CTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTATTGTTATTTTTCGT700490.2476763700146154No Hit
CTGTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGA700300.24760919059691813No Hit
GGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCC685460.24236212449887692No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT676940.23934965797897723No Hit
CTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCA671800.23753227794232412No Hit
CTCTGGTCCGTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAAT670210.23697009228896257No Hit
CACAGTTATCCAAGTAGGAGAGGAGCGAGCGACCAAAGGAACCATAACTG666940.2358138991528039No Hit
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCG661120.23375608751597102No Hit
GTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCAACTAAGAACGGC656760.23221449666775948No Hit
CTCTCATGTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCT646670.22864691601215056No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAACCGCGGTC640440.22644413826344456No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG636180.22493790500349473No Hit
ATCTGATAAATGCACGCATCCCCCCCGCGAAGGGGGTCAGCGCCCGTCGG632050.22347763660828515No Hit
ATCGGAATTAACCAGACAAATCGCTCCACCAACTAAGAACGGCCATGCAC576300.20376578115236885No Hit
CGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCC538370.19035464792469342No Hit
CAGCTTTGCAACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCCGG503600.17806081448608876No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA499720.17668894006153352No Hit
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC499400.176575795779096No Hit
CTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGC497770.1759994670904297No Hit
CCCGAGGTTATCTAGAGTCACCAAAGCCGCCGGCGCCCGCCCCCCGGCCG493910.1746346641835268No Hit
CTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACCATACTCCCCCCGGA492360.17408662156546995No Hit
CTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAAC471640.16676052927763882No Hit
CCCGAAGTTACGGATCCGGCTTGCCGACTTCCCTTACCTACATTGTTCCA464310.16416881805805375No Hit
CCACAGTTATCCAAGTAGGAGAGGAGCGAGCGACCAAAGGAACCATAACT463270.16380109914013175No Hit
ACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCT463210.16377988458717468No Hit
CTAGAATTACCACAGTTATCCAAGTAGGAGAGGAGCGAGCGACCAAAGGA461320.16311162616902794No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC460760.16291362367476223No Hit
CACCAGACTTGCCCTCCAATGGATCCTCGTTAAAGGATTTAAAGTGGACT453850.16047041432587647No Hit
CCCAGGCATAGTTCACCATCTTTCGGGTCCTAACACGTGCGCTCGTGCTC453140.16021937544921816No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA452520.1600001584019954No Hit
CGTTATCGGAATTAACCAGACAAATCGCTCCACCAACTAAGAACGGCCAT451210.15953697399576672No Hit
CTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATC449560.1589535737894481No Hit
CTCTAAGAAGTTGGGGGACGCCGACCGCTCGGGGGTCGCGTAACTAGTT448320.1585151396950026No Hit
GCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGG448110.15844088875965298No Hit
CCGTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAG429030.15169466091931427No Hit
CGTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAG428270.1514259432485251No Hit
CTCGTGCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTG420890.1488165532348092No Hit
GTTCGATTAGTCTTTCGCCCCTATACCCAGGTCGGACGACCGATTTGCAC419610.148363976105059No Hit
CTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGTCCCTTCGG413630.14624959232700735No Hit
CCCGCACTTACTGGGAATTCCTCGTTCATGGGGAATAATTGCAATCCCCG411940.14565204908538404No Hit
CTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTC405850.1434987719602445No Hit
CTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTT404780.14312044576584396No Hit
GCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCG402830.14243097279474015No Hit
CGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAACC402360.14226479212991No Hit
CTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGGGCTTCTTACCCAT401900.14210214722390604No Hit
GTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCGT400500.14160714098824176No Hit
CTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC397080.1403979114696905No Hit
CACCAACTAAGAACGGCCATGCACCACCACCCACGGAATCGAGAAAGAGC395530.1398498688516336No Hit
CCCGGAAGCTGCCCGGCGGGTCATGGGAATAACGCCGCCGCATCGCCGGT392410.13874671209786754No Hit
CTCCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACT389290.13764355534410147No Hit
CCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGGA387460.13699651147891173No Hit
CCTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGA387350.13695761813182383No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG382650.13529581148352238No Hit
GTTGGTTTTGATCTGATAAATGCACGCATCCCCCCCGCGAAGGGGGTCAG382040.1350801301951258No Hit
CTCCACTTCGGCCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCA375200.13266167115802324No Hit
CTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCCCAAGCAACCCGA374920.13256266991089038No Hit
CTCGTTTGAATATTTGCTACTACCACCAAGATCTGCACCTGCGGCGGCTC370190.1308902559861104No Hit
CTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTG352360.12458599799904337No Hit
CACCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAA348860.12334848240988269No Hit
GTCCTAACACGTGCGCTCGTGCTCCACCTCCCCGGCGCGGCGGGCGAGAC343580.12148160174966319No Hit
CTCCCGTCCACTCTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCT332560.11758519552322018No Hit
CTGGGGTCTGATGAGCGTCGGCATCGGGCGCCTTAACCCGGCGTTCGGTT327890.11593399615139723No Hit
CCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTG325150.11496519823302576No Hit
CGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTC323520.11438886954435949No Hit
CTTCGATGTCGGCTCTTCCTATCATTGTGAAGCAGAATTCACCAAGCGTT318970.11278009927845063No Hit
GTCAATTCCTTTAAGTTTCAGCTTTGCAACCATACTCCCCCCGGAACCCA316690.1119739462660831No Hit
CTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTCCGC316070.11175472921886036No Hit
CTCTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATGGTAG315950.11171230011294629No Hit
CCTCGTTCATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGG315440.11153197641281143No Hit
CTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGT314330.1111395071831062No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG310760.10987724128216232No Hit
CTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGTT306650.10842404440460508No Hit
CGTCAATTCCTTTAAGTTTCAGCTTTGCAACCATACTCCCCCCGGAACCC305320.10795378848072403No Hit
CTTGTGTCGAGGGCTGACTTTCAATAGATCGCAGCGAGGGAGCTGCTCTG304570.10768860656876103No Hit
CCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGA299940.10605155023224277No Hit
CACCATCTTTCGGGTCCTAACACGTGCGCTCGTGCTCCACCTCCCCGGCG295640.10453117393698826No Hit
CTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATCGGTC293140.10364723423044492No Hit
ACCAGACTTGCCCTCCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTC290410.1026819720708996No Hit
CTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAAC290320.10265015024146405No Hit
CTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCTTCTTTCCCC284220.10049333735749832No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)