FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005203728

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005203728
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26918972
Sequences flagged as poor quality0
Sequence length101
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCGATGTCGGCTCTTCCTATCATTGTGAAGCAGAATTCACCAAGCGTT2387960.887091825051863No Hit
CTTCGTCCTGGGAAACGGGGCGCGGCCGGAAAGGCGGCCGCCCCCTCGCC1345060.499669898241285No Hit
CTCGGATCGGCCCCGCCGGGGTCGGCCCACGGCCCTGGCGGAGCGCTGAG1314850.4884473300094818No Hit
CTCTTTTCTTTGTGAAGGGCAGGGCGCCCTGGAATGGGTTCGCCCCGAGA1185080.440239694145824No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT1145260.42544715303392716No Hit
CTGATGATGTGTTGTTGCCATGGTAATCCTGCTCAGTACGAGAGGAACCG1018690.37842826984626304No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA950820.35321556855885883No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT839000.31167609223710324No Hit
CGACTGTTTAATTAAAACAAAGCATCGCGAAGGCCCGCGGCGGGTGTTGA829290.3080689708358848No Hit
CTCCGAAGTTTCCCTCAGGATAGCTGGCGCTCTCGCAGACCCGACGCACC736440.27357656897150456No Hit
CTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCAGTACGAGAGGAA721850.2681565997393957No Hit
GTTGGATTGTTCACCCACTAATAGGGAACGTGAGCTGGGTTTAGACCGTC695250.2582750931201979No Hit
GGGAGTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCT689770.25623935416255866No Hit
CAGAAAAGTTACCACAGGGATAACTGGCTTGTGGCGGCCAAGCGTTCATA686250.25493172621896554No Hit
CTTTTCTTTGTGAAGGGCAGGGCGCCCTGGAATGGGTTCGCCCCGAGAGA634900.23585596062137884No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT623000.2314352866075272No Hit
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC598770.22243419993898728No Hit
CTGGGTTTAGACCGTCGTGAGACAGGTTAGTTTTACCCTACTGATGATGT597230.2218621127136653No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG593610.22051733624894743No Hit
GCCCTTCGTCCTGGGAAACGGGGCGCGGCCGGAAAGGCGGCCGCCCCCTC551570.20490009796807993No Hit
CCTAAGGCGAGCTCAGGGAGGACAGAAACCTCCCGTGGAGCAGAAGGGCA531760.1975409759332563No Hit
CTCGGGGCCACGCGCGCGTCCCCCGAAGAGGGGGACGGCGGAGCGAGCGC527930.19611818757417632No Hit
GTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGCCGGGGGCATT515200.1913891808349888No Hit
CTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTG492730.1830419081382454No Hit
CTTGCGTTGATTAAGTCCCTGCCCTTTGTACACACCGCCCGTCGCTACTA474940.1764331862301428No Hit
GTCGCTTTTTGATCCTTCGATGTCGGCTCTTCCTATCATTGTGAAGCAGA470690.17485437408233864No Hit
CTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGC459930.17085719320930975No Hit
AGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAAGGCTTCCA452940.168260511582686No Hit
GTTTTAAGCAGGAGGTGTCAGAAAAGTTACCACAGGGATAACTGGCTTGT451300.16765127583623923No Hit
CTCGATTCCGTGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGCGAT449010.16680057470248122No Hit
CTGCCCTTTGTACACACCGCCCGTCGCTACTACCGATTGGATGGTTTAGT432840.16079365883660046No Hit
CCTTCGTCCTGGGAAACGGGGCGCGGCCGGAAAGGCGGCCGCCCCCTCGC430890.16006926267466678No Hit
CTCGTAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGCC426330.15837529011137574No Hit
GGCGTACGGAAGACCCGCTCCCCGGCGCCGCTCGTGGGGGGCCCAAGTCC414170.1538580299425996No Hit
GTTGTTGCCATGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGA406650.15106446115401437No Hit
CTTGATTTTCAGTACGAATACAGACCGTGAAAGCGGGGCCTCACGATCCT402190.1494076371118481No Hit
CCTGCCCTTTGTACACACCGCCCGTCGCTACTACCGATTGGATGGTTTAG397580.14769509028799466No Hit
CTCGCTTGATCTTGATTTTCAGTACGAATACAGACCGTGAAAGCGGGGCC392710.14588595730921672No Hit
CTCAGGATAGCTGGCGCTCTCGCAGACCCGACGCACCCCCGCCACGCAGT389820.14481236504870987No Hit
GGGGAGTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCC374140.13898747693634067No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG372120.1382370768096196No Hit
CTCCTGCCGACAAGACCAACGTCAAGGCCGCCTGGGGTAAGGTCGGCGCG371270.1379213143800588No Hit
CTGGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGC363530.135046018844999No Hit
TGATGATGTGTTGTTGCCATGGTAATCCTGCTCAGTACGAGAGGAACCGC356500.1324344778099253No Hit
CCAGTAAGTGCGGGTCATAAGCTTGCGTTGATTAAGTCCCTGCCCTTTGT350650.13026128932412426No Hit
CCGAAAGATGGTGAACTATGCCTGGGCAGGGCGAAGCCAGAGGAAACTCT349950.13000124967625065No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT348190.1293474357044541No Hit
GTCCTAAGGCGAGCTCAGGGAGGACAGAAACCTCCCGTGGAGCAGAAGGG346500.1287196256974449No Hit
GTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTG329800.12251582266960268No Hit
CTGAGAGATGGGCGAGCGCCGTTCCGAAGGGACGGGCGATGGCCTCCGTT326120.12114875709220992No Hit
GTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTGCCTACCC317170.11782396445153998No Hit
CTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGGCGAGCT316680.11764193669802844No Hit
GGTGTATGTGCTTGGCTGAGGAGCCAATGGGGCGAAGCTACCATCTGTGG314240.11673551278258323No Hit
GTCGGCTCTTCCTATCATTGTGAAGCAGAATTCACCAAGCGTTGGATTGT309320.11490780554324288No Hit
CGTTGATTAAGTCCCTGCCCTTTGTACACACCGCCCGTCGCTACTACCGA306200.113748771684149No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA305650.11354445481796258No Hit
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC299450.11124124650822476No Hit
CTCTTTCTCGATTCCGTGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGG291280.1082062123323283No Hit
CTTGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGG291190.10817277866331597No Hit
CTTTTGGGTTTTAAGCAGGAGGTGTCAGAAAAGTTACCACAGGGATAACT289700.1076192656985564No Hit
GCTCTTTCTCGATTCCGTGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTG287970.10697659628309727No Hit
GCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTTTACCCTACTGATGATG282290.10486656028320844No Hit
CTCAACACGGGAAACCTCACCCGGCCCGGACACGGACAGGATTGACAGAT276520.10272309061430726No Hit
GTCCACTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGC274280.10189096374111166No Hit
GTTTAATTAAAACAAAGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGA270640.10053875757216879No Hit
CTTGAAAATCCGGGGGAGAGGGTGTAAATCTCGCGCCGGGCCGTACCCAT269270.10002982283275899No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)