FastQCFastQC Report
Fri 1 Jul 2022
EGAF00005203811

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005203811
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61994596
Sequences flagged as poor quality0
Sequence length101
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAACCTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCG3854980.6218251668258311No Hit
CCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTT2209360.35637944958944484No Hit
CCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTT2078520.3352743842382649No Hit
CTCAGACTTTATTCAAAGACCAGGAAGGGCCGGTGCAAGGAGGGGAGGAG1894300.30555889097172273No Hit
CTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCAT1797230.2899010746033412No Hit
CGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAA1783590.28770088283178746No Hit
CTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC1767630.2851264648938111No Hit
CTCGTACTGAGCAGGATTACCATGGCAACAACACATCATCAGTAGGGTAA1650350.26620868696361855No Hit
CTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTC1644950.26533764330039344No Hit
GTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGG1559810.25160418821021113No Hit
CTGACTTAGAGGCGTTCAGTCATAATCCCACAGATGGTAGCTTCGCCCCA1489950.24033546407819156No Hit
CGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCT1474190.23779330701663096No Hit
CGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCT1343510.21671405036658356No Hit
CAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAG1343170.2166592068766768No Hit
CCTTCCGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTACTTCCTCT1299290.20958117059106252No Hit
CCTCAGCCAAGCACATACACCAAATGTCTGAACCTGCGGTTCCTCTCGTA1286620.20753744407012506No Hit
CTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGGACGCCGACCGCTCGGG1263840.20386293024637178No Hit
CGCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGT1221160.19697845921925194No Hit
CACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACCACCA1155290.18635333957172656No Hit
GTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCA1121890.18096577321029722No Hit
CTAGAGTCAAGCTCAACAGGGTCTTCTTTCCCCGCTGATTCCGCCAAGCC1099100.17728964634272315No Hit
CTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGG1097150.17697510279766965No Hit
CTGGAGGATCGCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCG1090270.17586532864896806No Hit
ACCGAGGCTCCGCGGCGCTGCCGTATCGTTCCGCCTGGGCGGGATTCTGA1080720.17432487180011627No Hit
CTTCGCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACC1070460.17266988883998857No Hit
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGG1039920.1677436530113044No Hit
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGG1010420.16298517373998211No Hit
CACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAACGGAGCA1004970.16210606485765308No Hit
CTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTCCGC981520.15832347709790706No Hit
CTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC946620.15269395416335965No Hit
CTCTAATCATTCGCTTTACCGGATAAAACTGCGTGGCGGGGGTGCGTCGG942860.15208744968674368No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGA916470.14783062704368619No Hit
CACAGATGGTAGCTTCGCCCCATTGGCTCCTCAGCCAAGCACATACACCA910760.1469095790220167No Hit
CGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGT903160.14568366571821842No Hit
CTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACCACCAGG886040.1429221346970307No Hit
CCGAGGCTCCGCGGCGCTGCCGTATCGTTCCGCCTGGGCGGGATTCTGAC803340.12958226229912037No Hit
GATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGG779230.1256932136472024No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGA770100.12422050463882367No Hit
CTTCCGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTACTTCCTCTA765360.12345592186777053No Hit
CGAGGCTCCGCGGCGCTGCCGTATCGTTCCGCCTGGGCGGGATTCTGACT764050.12324461312724741No Hit
CTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCT761940.12290426088106131No Hit
GGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGA751860.12127831270970779No Hit
CTTAGAGGCGTTCAGTCATAATCCCACAGATGGTAGCTTCGCCCCATTGG746650.12043791687907765No Hit
CAAAGACTTTGGTTTCCCGGAAGCTGCCCGGCGGGTCATGGGAATAACGC744280.12005562549355109No Hit
CGAAGGATCAAAAAGCGACGTCGCTATGAACGCTTGGCCGCCACAAGCCA739890.1193474992562255No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGA727790.11739571623307296No Hit
ATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGT723540.1167101726092384No Hit
CATAATCCCACAGATGGTAGCTTCGCCCCATTGGCTCCTCAGCCAAGCAC720060.11614883335960445No Hit
CTCAGCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGG716080.11550684191893112No Hit
CTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAAGGATCGTGAG713880.11515197227835794No Hit
CTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCAAT711860.11482613742655892No Hit
CGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTA707410.11410833292630862No Hit
GTCATAATCCCACAGATGGTAGCTTCGCCCCATTGGCTCCTCAGCCAAGC704790.11368571544526236No Hit
CTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGCCGACTTCC661730.1067399487529526No Hit
CGCAGGGCCGGCGGACCCCGCCCCGGGCCCCTCGCGGGGACACCGGGGGG648270.10456879177017299No Hit
CAACAACACATCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTA640760.10335739586076181No Hit
CATCAATATGGTGACCTCCCGGGAGCGGGGGACCACCAGGTTGCCTAAGG636620.10268959571895589No Hit
CCTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCG635900.10257345656385922No Hit
CGAAACCCCGACCCAGAAGCAGGTCGTCTACGAATGGTTTAGCGCCAGGT623720.10060876919014038No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)