FastQCFastQC Report
Mon 13 May 2024
EGAF00005203842

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005203842
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences89773410
Total Bases9 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACGATATGATCTCGTAT3169810.35309007422130895TruSeq Adapter, Index 15 (97% over 36bp)
ACACTCTTTCCCTACACGACGCTCTTCCGATCAGATCGGAAGAGCACACG902140.1004907800650549Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)