FastQCFastQC Report
Mon 13 May 2024
EGAF00005203846

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005203846
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences91334907
Total Bases9.2 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTTGTTTATCTCGTAT3849970.4215222992453476TruSeq Adapter, Index 8 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTTGTTTATCGCGTAT1387520.15191563067995462TruSeq Adapter, Index 8 (97% over 38bp)
ACACTCTTTCCCTACACGACGCTCTTCCGATCAGATCGGAAGAGCACACG1000580.10955066719452618Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)