FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005203855

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005203855
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44121523
Sequences flagged as poor quality0
Sequence length35-51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGGC121200.020.9402621
GGCGATC41800.019.1668822
GGTGGCG145250.017.2063542
GGGCGAT47000.017.0997281
GGCGCGT147150.016.8468115
TGGCGCG149250.016.6529884
GCGCGTG156100.015.8679016
TCGCGCT14150.015.04733345
GTGGCGC178900.013.9056483
CGATCTG73200.013.7165651
GCGACAT129200.013.6085847
TGCGACA131450.013.3749976
CTGCGAC134400.013.0803865
CGACATC137550.013.02568158
TGCGCGA11600.012.85553911
GTACCGT47350.012.82671545
CGCGTGC194850.012.6557467
GCTGCGA143100.012.3151274
CGGGGTT53350.011.851241
GGCTGCG148500.011.8075433
Produced by FastQC (version 0.11.5)