FastQCFastQC Report
Tue 28 Jun 2022
EGAF00005203898

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005203898
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences102747673
Sequences flagged as poor quality0
Sequence length101
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCCGCAGATCTCGTAT11546081.1237315321000019TruSeq Adapter, Index 18 (97% over 40bp)
GGCGCCAGAAGCGAGAGCCCCTCGGGGCTCGCCCCCCCGCCTCACCGGGT5340300.5197489971378719No Hit
GTCGGATTCCCCTGGTCCGCACCAGTTCTAAGTCGGCTGCTAGGCGCCGG4527210.44061435824439543No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCCGCAGATCGCGTAT3420120.3328659326425816TruSeq Adapter, Index 18 (97% over 40bp)
TGGCGCCAGAAGCGAGAGCCCCTCGGGGCTCGCCCCCCCGCCTCACCGGG3390660.32999871442344003No Hit
ACACTCTTTCCCTACACGACGCTCTTCCGATCAGATCGGAAGAGCACACG3178690.30936856350994923Illumina Single End PCR Primer 1 (96% over 32bp)
GTCGGCTGCTAGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAACCGCGGCC2817520.2742174024710029No Hit
CTACGAATGGTTTAGCGCCAGGTTCCCCACGAACGTGCGGTGCGTGACGG2730530.26575103068270944No Hit
GCCAGAAGCGAGAGCCCCTCGGGGCTCGCCCCCCCGCCTCACCGGGTCAG2383300.2319565913672809No Hit
GCGCCAGAAGCGAGAGCCCCTCGGGGCTCGCCCCCCCGCCTCACCGGGTC2356200.22931906204824706No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC2336340.2273861715583573No Hit
CCCCAGTCAAACTCCCCACCTGGCACTGTCCCCGGAGCGGGTCGCGCCCG2308120.22463963733757747No Hit
GGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCC2304390.22427661208444105No Hit
CCCTTCTGCTCCACGGGAGGTTTCTGTCCTCCCTGAGCTCGCCTTAGGAC2287330.22261623384891646No Hit
CCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGGA2165850.21079309504167554No Hit
GTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCC2164340.21064613307592864No Hit
GGGGAGAGCGCGGCGACGGGTCTCGCTCCCTCGGCCCCGGGATTCGGCGA2040370.19858065301391303No Hit
CCCCGCCTCACCGGGTCAGTGAAAAAACGATCAGAGTAGTGGTATTTCAC1997860.19444333303781974No Hit
CCGGGATCGGTCGCGTTACCGCACTGGACGCCTCGCGGCGCCCATCTCCG1764030.17168564002417844No Hit
GTCGTCTACGAATGGTTTAGCGCCAGGTTCCCCACGAACGTGCGGTGCGT1761400.1714296731566855No Hit
GCCAGGTTCCCCACGAACGTGCGGTGCGTGACGGGCGAGGGGGCGGCCGC1741050.1694490930222819No Hit
GGGGGGAGAGCGCGGCGACGGGTCTCGCTCCCTCGGCCCCGGGATTCGGC1727810.1681604993623554No Hit
CCCGAAGTTACGGATCCGGCTTGCCGACTTCCCTTACCTACATTGTTCCA1712140.16663540399596202No Hit
GCTACTACCACCAAGATCTGCACCTGCGGCGGCTCCACCCGGGCCCGCGC1653060.16088539542885805No Hit
GCGGTGCGTGACGGGCGAGGGGGCGGCCGCCTTTCCGGCCGCGCCCCGTT1650270.16061385643254422No Hit
GCAGAAATCACATCGCGTCAACACCCGCCGCGGGCCTTCGCGATGCTTTG1616420.1573193779288802No Hit
CTCCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACT1613120.15699820277194987No Hit
GTTCGATTAGTCTTTCGCCCCTATACCCAGGTCGGACGACCGATTTGCAC1601880.1559042607222842No Hit
GCAGAATCCAGATGCTCAAGGCCCTTCATAATATCCCCCAGTTTAGTAGT1600160.15573686033745993No Hit
CGGCGCGAGATTTACACCCTCTCCCCCGGATTTTCAAGGGCCAGCGAGAG1536480.14953915306675608No Hit
CCCCAGTCAAACTCCCCACCTGGCACTGTCCCCGGAGCGGGTCGCGCCCC1503120.14629236420760594No Hit
CGGGTGGGTAGCCGACGTCGCCGCCGACCCCGTGCGCTCGCTCCGCCGTC1489830.14499890425742293No Hit
GGCAGAAATCACATCGCGTCAACACCCGCCGCGGGCCTTCGCGATGCTTT1487830.14480425264716212No Hit
CTCCTACTCGTCGCGGCGTAGCGTCCGCGGGGCTCCGGGGGCGGGGAGCG1486900.14471373964839088No Hit
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC1390590.1353402913562821No Hit
GCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGGACGCCTCGCGGCG1363990.13275142493981348No Hit
CCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGGACGCCTCGCGGCGC1330550.12949685001625294No Hit
CGGCCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCAAGATCTGC1319380.12840972077294635No Hit
GCCCCAGTCAAACTCCCCACCTGGCACTGTCCCCGGAGCGGGTCGCGCCC1315770.1280583746164256No Hit
GTCGCGTTACCGCACTGGACGCCTCGCGGCGCCCATCTCCGCCACTCCGG1298750.1264018894131062No Hit
GGGTAGCCGACGTCGCCGCCGACCCCGTGCGCTCGCTCCGCCGTCCCCCT1247970.12145968502858455No Hit
GCCCTTCTGCTCCACGGGAGGTTTCTGTCCTCCCTGAGCTCGCCTTAGGA1222970.11902653990032457No Hit
CCGACATCGAAGGATCAAAAAGCGACGTCGCTATGAACGCTTGGCCGCCA1214490.11820121707281878No Hit
GGCGGGGGTGCGTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACT1210220.11778563588491196No Hit
CCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTG1206450.11741871759957036No Hit
GTCAAACTCCCCACCTGGCACTGTCCCCGGAGCGGGTCGCGCCCGGCCGG1206300.1174041187288008No Hit
CCCACGAACGTGCGGTGCGTGACGGGCGAGGGGGCGGCCGCCTTTCCGGC1186030.11543132465880761No Hit
CGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGGACG1176360.11449018412319666No Hit
CCCTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGA1143360.11127843255389347No Hit
CTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGTCCCTTCGG1135650.11052805059633808No Hit
CCCGAGAGAACCTCCCCCGGGCCCGACGGCGCGACCCGCCCGGGGCGCAC1130270.11000443776473653No Hit
CTCCACTTCGGCCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCA1114630.10848226217249708No Hit
GTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACT1109550.10798784708243465No Hit
GCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCATCCATTCATGCGC1107860.10782336647176427No Hit
CGAATGGTTTAGCGCCAGGTTCCCCACGAACGTGCGGTGCGTGACGGGCG1105020.10754696118519394No Hit
GCGTGACGGGCGAGGGGGCGGCCGCCTTTCCGGCCGCGCCCCGTTTCCCA1101050.10716057773882627No Hit
CCCTGGTCCGCACCAGTTCTAAGTCGGCTGCTAGGCGCCGGCCGAGGCGA1094830.10655521123091517No Hit
CCCGGCGCGAGATTTACACCCTCTCCCCCGGATTTTCAAGGGCCAGCGAG1086010.10569679762966505No Hit
CTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTCCGC1070310.10416878248911779No Hit
CCAGAAGCGAGAGCCCCTCGGGGCTCGCCCCCCCGCCTCACCGGGTCAGT1067330.10387875158982919No Hit
CCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGGAC1067180.10386415271905963No Hit
CCACGAACGTGCGGTGCGTGACGGGCGAGGGGGCGGCCGCCTTTCCGGCC1043110.10152152058957092No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)