FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005204001

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005204001
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53879354
Sequences flagged as poor quality0
Sequence length35-51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAACCTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGT1809270.3358002399212136No Hit
CTCGTACTGAGCAGGATTACCATGGCAACAACACATCATCAGTAGGGTAA1676440.3111470118962451No Hit
ACCGAGGCTCCGCGGCGCTGCCGTATCGTTCCGCCTGGGCGGGATTCTGA1557350.28904392580504956No Hit
CCTCAGCCAAGCACATACACCAAATGTCTGAACCTGCGGTTCCTCTCGTA1446320.2684367745017878No Hit
CTAGAGTCAAGCTCAACAGGGTCTTCTTTCCCCGCTGATTCCGCCAAGCC1242310.23057254918089776No Hit
CTTCGCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAAC1162380.21573755320080487No Hit
CCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTT1126960.20916360652727944No Hit
GTCATAATCCCACAGATGGTAGCTTCGCCCCATTGGCTCCTCAGCCAAGC1105740.205225177718352No Hit
CGAGGCTCCGCGGCGCTGCCGTATCGTTCCGCCTGGGCGGGATTCTGACTT1103270.20476674608979167No Hit
CTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTC973000.18058865367984925No Hit
CCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTT963440.17881431911748608No Hit
CCGAGGCTCCGCGGCGCTGCCGTATCGTTCCGCCTGGGCGGGATTCTGA946470.17566468966944182No Hit
CTCTAATCATTCGCTTTACCGGATAAAACTGCGTGGCGGGGGTGCGTCGGG933130.17318878767551668No Hit
CATAATCCCACAGATGGTAGCTTCGCCCCATTGGCTCCTCAGCCAAGCAC909680.16883647120193757No Hit
GTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCAC905250.16801426386812285No Hit
ACTAGAGTCAAGCTCAACAGGGTCTTCTTTCCCCGCTGATTCCGCCAAGC898540.16676888887717548No Hit
CTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGG895510.16620652133282815No Hit
CGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAA879320.16320165976748718No Hit
CTGACTTAGAGGCGTTCAGTCATAATCCCACAGATGGTAGCTTCGCCCCAT873330.16208991666826594No Hit
CTCAGACTTTATTCAAAGACCAGGAAGGGCCGGTGCAAGGAGGGGAGGAGG868920.1612714213314436No Hit
CACAGATGGTAGCTTCGCCCCATTGGCTCCTCAGCCAAGCACATACACCA860260.15966412663373802No Hit
CAAAGACTTTGGTTTCCCGGAAGCTGCCCGGCGGGTCATGGGAATAACGC837530.155445442051885No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGAT832310.15447661083687084No Hit
CTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGGACGCCGACCGCTCGGGG820730.15232736457827611No Hit
CTTAGAGGCGTTCAGTCATAATCCCACAGATGGTAGCTTCGCCCCATTGG818050.15182995698129564No Hit
CGAAGGATCAAAAAGCGACGTCGCTATGAACGCTTGGCCGCCACAAGCC812240.1507516218550059No Hit
CCTTCCGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTACTTCCTCT804250.14926867905654548No Hit
CTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGCCGACTTCC792440.14707674483253827No Hit
CGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCACAGATGGTAGCTT769760.14286734024316622No Hit
CAAAAGGTCAGAAGGATCGTGAGGCCCCGCTTTCACGGTCTGTATTCGTA761050.14125076555297972No Hit
GTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGG755760.14026894234849216No Hit
CTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCG750080.13921473520265296No Hit
CTTATCCCGAAGTTACGGATCCGGCTTGCCGACTTCCCTTACCTACATTGT736440.13668315325384192No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGAT729190.13533755434409997No Hit
CGCAGGGCCGGCGGACCCCGCCCCGGGCCCCTCGCGGGGACACCGGGGGGG724990.13455803497569774No Hit
GGTAGCTTCGCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGT691780.12839426396983156No Hit
GTGGGAATCTCGTTCATCCATTCATGCGCGTCACTAATTAGATGACGAGG672380.12479362688721175No Hit
CCACAAGCCAGTTATCCCTGTGGTAACTTTTCTGACACCTCCTGCTTAAA649430.12053411033844244No Hit
CTTGGCTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTT638800.11856118393698634No Hit
GGAAGAGCCGACATCGAAGGATCAAAAAGCGACGTCGCTATGAACGCTTGG629830.1168963532858987No Hit
CCCGAAGTTACGGATCCGGCTTGCCGACTTCCCTTACCTACATTGTTCCA625450.11608342594456496No Hit
CTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAAGGATCGTGAGG616120.1143517793476143No Hit
ACCAAATGTCTGAACCTGCGGTTCCTCTCGTACTGAGCAGGATTACCATGG613500.11386550774161101No Hit
GTTCAGTCATAATCCCACAGATGGTAGCTTCGCCCCATTGGCTCCTCAGC600430.11143971770708314No Hit
GGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTAT600000.1113599097717467No Hit
TGTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCTTCTTTC598280.11104067803040105No Hit
CACCAAATGTCTGAACCTGCGGTTCCTCTCGTACTGAGCAGGATTACCATG593470.11014794275373085No Hit
CAACAACACATCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTA584390.10846269611918509No Hit
CAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGT575400.10679415347110507No Hit
CTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCTTCTTTCCCCG573280.10640068178991158No Hit
CTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCAT571590.10608701804405452No Hit
GTCTGAACCTGCGGTTCCTCTCGTACTGAGCAGGATTACCATGGCAACAA571320.10603690608465721No Hit
GCCAGTTATCCCTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAA569180.1056397224064713No Hit
GTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGAT556010.10319537238698147No Hit
CGTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCATAAT552330.10251236494038143No Hit
CAAATGTCTGAACCTGCGGTTCCTCTCGTACTGAGCAGGATTACCATGGC547690.10165118163814658No Hit
GTCAAGCTCAACAGGGTCTTCTTTCCCCGCTGATTCCGCCAAGCCCGTTC547370.10159178968626832No Hit
CATACACCAAATGTCTGAACCTGCGGTTCCTCTCGTACTGAGCAGGATTA546980.10151940574491668No Hit
CTTGGAGACCTGCTGCGGATATGGGTACGGCCCGGCGCGAGATTTACACC542760.10073617437952208No Hit
CTCAGCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGG542220.10063595046072751No Hit
CCTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCG540970.10040395064870303No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)