FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005204026

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005204026
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34630190
Sequences flagged as poor quality0
Sequence length35-51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGAT2028800.5858472044190344No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT1735070.5010281491380787No Hit
CTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCGTCTTG1716850.4957668439012319No Hit
CTCGTTTGAATATTTGCTACTACCACCAAGATCTGCACCTGCGGCGGCTC1639790.4735145836624055No Hit
CTCCACTTCGGCCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCA1460940.42186889531937305No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA1350110.38986502817339436No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAACCGCGGTC1266550.36573579295984227No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG1251160.361291693750453No Hit
CTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGTCCCTTCGG1192390.3443209523251244No Hit
CTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTG1120720.3236251374884169No Hit
CTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCAAGATCTGCAC1036520.29931109243119947No Hit
GTTCGATTAGTCTTTCGCCCCTATACCCAGGTCGGACGACCGATTTGCACG992380.2865649885259076No Hit
CTCCCGTCCACTCTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCT978580.28258002627187434No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTG959140.27696642727054055No Hit
CTCTCATGTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCTT942230.27208340468244613No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG939490.27129218753925405No Hit
CTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCA870150.2512691960396406No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT866500.2502152023999868No Hit
CTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATCG844730.243928780061559No Hit
GTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGG782590.22598489930318028No Hit
CTCCGACTTTCGTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCG741430.21409931623245498No Hit
CTCCTCTATCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGG729780.21073519954698489No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG711900.20557207453958526No Hit
CCCGAAGTTACGGATCCGGCTTGCCGACTTCCCTTACCTACATTGTTCCA705310.203669110680594No Hit
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCG701780.2026497688866275No Hit
CTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCTTCTTTCCCCG677360.19559811828927304No Hit
CCCATCTCTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAA676320.1952978022933169No Hit
CTGGGGTCTGATGAGCGTCGGCATCGGGCGCCTTAACCCGGCGTTCGGTT669910.19344681620285653No Hit
GTCCTAACACGTGCGCTCGTGCTCCACCTCCCCGGCGCGGCGGGCGAGACG669340.1932822199358421No Hit
CTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATCGGT655170.18919041449093985No Hit
CCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGC619890.1790027718588896No Hit
CTCTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGCGCCAT594950.17180096326355704No Hit
CTCGTGCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTG584720.16884689341871933No Hit
CTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCATCCATTCATGCGCGT580490.16762541585824392No Hit
CTAAGAAGTTGGGGGACGCCGACCGCTCGGGGGTCGCGTAACTAGTTAGC572580.165341281696693No Hit
CTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTT572490.16531529281242754No Hit
CTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGTTT564080.16288677596051307No Hit
CCCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCG559750.16163642186196495No Hit
CCACTCTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGCGC557660.16103290221624542No Hit
GGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCC549030.158540856980571No Hit
CTCGTTCATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTT548980.15852641871153464No Hit
CACAGTTATCCAAGTAGGAGAGGAGCGAGCGACCAAAGGAACCATAACTG542050.1565252746230962No Hit
CTCCGCCACTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGC541090.15624805985759824No Hit
CTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTATTGTTATTTTTCGT506070.14613549622453703No Hit
CCTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGA492800.1423035796222891No Hit
GTCCACTCTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGCG479090.1383446062525213No Hit
CGCCACTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCG467880.13510754633457106No Hit
CTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACCATACTCCCCCCGGA464000.13398713665735013No Hit
CTGGCTTCTGTGAGCACCGTGCTGACCTCCAAATACCGTTAAGCTGGAGC443390.12803568216056568No Hit
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTT437400.12630597753001066No Hit
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC437160.12623667383863618No Hit
CTCCCATCCAAGTACTAACCAGGCCCGACCCTGCTTAGCTTCCGAGATC430150.12421242851973957No Hit
CTGATGAGCGTCGGCATCGGGCGCCTTAACCCGGCGTTCGGTTCATCCCG425850.12297073738261326No Hit
CCCAGGCATAGTTCACCATCTTTCGGGTCCTAACACGTGCGCTCGTGCTC422210.12191963139676681No Hit
CTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCATCC419360.12109665006169472No Hit
GTTGGTTTTGATCTGATAAATGCACGCATCCCCCCCGCGAAGGGGGTCAG416620.12030543291850261No Hit
GTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCGT416150.1201697131895609No Hit
CTGCTGTCTATATCAACCAACACCTTTTCTGGGGTCTGATGAGCGTCGGC411450.11881251590014379No Hit
CCCTTACCTACATTGTTCCAACATGCCAGAGGCTGTTCACCTTGGAGAC409040.11811659133259159No Hit
CTCGTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCAACTAAGAACG408280.11789712964323903No Hit
CTGGTTAGTTTCTTCTCCTCCGCTGACTAATATGCTTAAATTCAGCGGGT395020.11406810069479838No Hit
CCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA394470.11390927973539851No Hit
CGGGCTTCTTACCCATTTAAAGTTTGAGAATAGGTTGAGATCGTTTCGGC388580.1122084516429162No Hit
CTGCCGTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTC388510.1121882380662653No Hit
CTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGTCCCTTCGGAACGG386720.11167134803476389No Hit
GTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGGAAC377970.10914465095340223No Hit
CCACAGTTATCCAAGTAGGAGAGGAGCGAGCGACCAAAGGAACCATAA375750.10850359180818818No Hit
CCCATTTAAAGTTTGAGAATAGGTTGAGATCGTTTCGGCCCCAAGACCT369020.10656020079589514No Hit
CTCCGGGATCGGTCGCGTTACCGCACTGGACGCCTCGCGGCGCCCATCTC368730.1064764588354843No Hit
CTCTGGTCCGTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAAT368490.10640715514410981No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC368100.10629453664562626No Hit
CTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCTTCTTTCCCCG367980.10625988479993902No Hit
CTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAA365120.10543401581105966No Hit
CTGATAAATGCACGCATCCCCCCCGCGAAGGGGGTCAGCGCCCGTCGGCAT355000.10251171015810193No Hit
CCCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACCGCG353860.10218251762407311No Hit
CTAACACGTGCGCTCGTGCTCCACCTCCCCGGCGCGGCGGGCGAGACGGG353740.10214786577838586No Hit
CCCGAGGTTATCTAGAGTCACCAAAGCCGCCGGCGCCCGCCCCCCGGCCGG350750.10128445729001198No Hit
CCCGTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGG348770.10071270183617242No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)