FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005204050

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005204050
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25266427
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAATTAACATCTCGTATG21113778.356452615955552TruSeq Adapter, Index 2 (97% over 37bp)
CCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTCA14597965.777611531697775No Hit
CCCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGC11280084.464453957023682No Hit
CCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCA7994023.1638901693539814No Hit
CTCCCATCCAAGTACTAACCAGGCCCGACCCTGCTTAGCTTCCGAGATCAG6791882.6881046536575988No Hit
ACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTC6680312.6439472427185686No Hit
CTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGG5267312.0847071095568834No Hit
GTTAAGACTCAGAGTTGTTCCTCTCCACGGAAATCTTTAGTAAAAGGCGAA4445241.7593465035638003No Hit
CCCACATTTGGGGAAATCGCAGGGGTCAGCACATCCGGAGTGCAATGGATA4294141.6995438254882655No Hit
GTCCCCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAAT3861441.5282889028986963No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAATTAACATCGCGTATG3418301.3529020149940472TruSeq Adapter, Index 2 (97% over 37bp)
CTCCTATTCCATCTCCCTGCTCCAAAAATCCATTTAATATATTGTCCTCGG3363151.3310746311696546No Hit
CACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTCAG3043251.2044639315246275No Hit
CCCCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCG2998201.186633947095092No Hit
GCCTACAGCACCCGGTATTCCCAGGCGGTCTCCCATCCAAGTACTAACCAG2709601.0724112277529387No Hit
CCACATTTGGGGAAATCGCAGGGGTCAGCACATCCGGAGTGCAATGGATAA2548401.0086111502825468No Hit
ATTGGGTTAAGACTCAGAGTTGTTCCTCTCCACGGAAATCTTTAGTAAAAG2486710.9841953514044546No Hit
GTCAAGTGCAGTAGTGAGAAGGGGGGAAAGAGTAGAACAAGGAGTTCGATC2187910.8659356544556142No Hit
GGTCGATGCGTGGAGTGGACGGAGCAAGCTCCTATTCCATCTCCCTGCTCC1970750.7799876096450044No Hit
GTGCAGTAGTGAGAAGGGGGGAAAGAGTAGAACAAGGAGTTCGATCTGTAA1930280.763970307317295No Hit
GTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTCAGCACATCCGGAGTG1900400.752144337622411No Hit
GTCGATGCGTGGAGTGGACGGAGCAAGCTCCTATTCCATCTCCCTGCTCCA1873590.7415334190307161No Hit
CATCCAAGTACTAACCAGGCCCGACCCTGCTTAGCTTCCGAGATCAGACGA1793870.7099816685596265No Hit
CCCGGTATTCCCAGGCGGTCTCCCATCCAAGTACTAACCAGGCCCGACCCT1683230.6661923349906181No Hit
CCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAG1324870.5243598550756702No Hit
GGCTAGTCAAGTGAAGCAGTGGGAGTGGAGAAGGAACAAAGAAATCTGTAA1223070.48406923543245745No Hit
GGTTAAGACTCAGAGTTGTTCCTCTCCACGGAAATCTTTAGTAAAAGGCGA1192050.47179207412270835No Hit
ATCCAAGTACTAACCAGGCCCGACCCTGCTTAGCTTCCGAGATCAGACGAG1178770.46653608759164883No Hit
CACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGG1175030.4650558624691967No Hit
ATTCCATCTCCCTGCTCCAAAAATCCATTTAATATATTGTCCTCGGATAGA1115240.44139204961587963No Hit
CTCCTATTTCCAAAAATCCATTTAATATATTGTCCTCGGATAGAGGACGTA1091350.4319368148096286No Hit
GCCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTC1075270.42557263834732156No Hit
CAGGTCGATGCGTGGAGTGGACGGAGCAAGCTCCTATTCCATCTCCCTGCT1004920.39772936632472805No Hit
CTAGTCAAGTGCAGTAGTGAGAAGGGGGGAAAGAGTAGAACAAGGAGTTCG990140.3918797066162145No Hit
CCGGTATTCCCAGGCGGTCTCCCATCCAAGTACTAACCAGGCCCGACCCTG987380.39078734796969905No Hit
CCCCCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATC966380.3824759234853428No Hit
CCTGGAGGTACTGCAATACCAGGTCGATGCGTGGAGTGGACGGAGCAAGCT960320.3800774838484286No Hit
ATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTCAGCACATCCG952990.37717640092126997No Hit
ACCAGGTCGATGCGTGGAGTGGACGGAGCAAGCTCCTATTCCATCTCCCTG947980.3751935325085735No Hit
CTGCAATACCAGGTCGATGCGTGGAGTGGACGGAGCAAGCTCCTATTCCAT923140.36536230468993497No Hit
CTCAAAAAATTGGGTTAAGACTCAGAGTTGTTCCTCTCCACGGAAATCTTT907220.3590614533665563No Hit
GTCGATGCGTGGAGTGGACGGAGCAAGCTCCTATTCCATCTCCTATTTCCA883040.34949144174599756No Hit
GCAGTAGTGAGAAGGGGGGAAAGAGTAGAACAAGGAGTTCGATCTGTAACT734510.29070592371450066No Hit
CTACAGCACCCGGTATTCCCAGGCGGTCTCCCATCCAAGTACTAACCAGGC729570.28875076005008543No Hit
GGTCGATGCGTGGAGTGGACGGAGCAAGCTCCTATTCCATCTCCTATTTCC717580.28400533245163634No Hit
CCCAGGCGGTCTCCCATCCAAGTACTAACCAGGCCCGACCCTGCTTAGCTT698340.2763904844954928No Hit
GTCAAATTTAGCAGTGGGGGGTTGTATACCAACTTTAGTGACACTAATGTT679870.26908038877044227No Hit
GCTCCTATTCCATCTCCCTGCTCCAAAAATCCATTTAATATATTGTCCTCG677210.2680276083357572No Hit
CTAGTCAAGTGAAGCAGTGGGAGTGGAGAAGGAACAAAGAAATCTGTAACT644860.2552240568086655No Hit
CCGCAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTCA626100.24779918426930725No Hit
CCCATCCAAGTACTAACCAGGCCCGACCCTGCTTAGCTTCCGAGATCAGAC625390.24751817896531236No Hit
CCTGGAAGTACTGCAATACCAGGTCGATGCGTGGAGTGGACGGAGCAAGCT619100.2450287094411885No Hit
GGCCTCAAAAAATTGGGTTAAGACTCAGAGTTGTTCCTCTCCACGGAAATC616650.24405904325134695No Hit
GTCAAGTGAAGCAGTGGGAGTGGAGAAGGAACAAAGAAATCTGTAACTGGT598020.236685622387368No Hit
CTCCCGTCCAAGTACTAACCAGGCCCGACCCTGCTTAGCTTCCGAGATCAG592330.23443362213422578No Hit
CTCCTATTCCATCTCCTATTTCCAAAAATCCATTTAATATATTGTCCTCGG585140.23158794870362953No Hit
CCCCGCTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGC558500.22104431307204617No Hit
CGATGCGTGGAGTGGACGGAGCAAGCTCCTATTCCATCTCCCTGCTCCAAA509360.2015955797786525No Hit
GTCAAAAATTGCCAATGCCGACTATATTTCAAGTCGTCATGGCGGGGTATT508550.20127499626282735No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT498450.19727759686797028No Hit
CTCAAAAAATTAGCTTAAGACTCAGAGTTGTTCCTCTCCACGGAAATCTTT484470.19174456285409883No Hit
GACTAGTCAAGTGCAGTAGTGAGAAGGGGGGAAAGAGTAGAACAAGGAGTT473350.18734346569857305No Hit
CCATCTCCCTGCTCCAAAAATCCATTTAATATATTGTCCTCGGATAGAGGA460780.18236848447150839No Hit
CCAAGTACTAACCAGGCCCGACCCTGCTTAGCTTCCGAGATCAGACGAGAT458820.18159275151963514No Hit
CCTACAGCACCCGGTATTCCCAGGCGGTCTCCCATCCAAGTACTAACCAGG453860.17962967221285384No Hit
CTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCGAA436370.17270744296373997No Hit
ACCGCAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTC433080.17140531979452417No Hit
CTGGAGGTACTGCAATACCAGGTCGATGCGTGGAGTGGACGGAGCAAGCTC427080.1690306270847081No Hit
AGCCAGTCAAATTTAGCAGTGGGGGGTTGTATACCAACTTTAGTGACACTA427060.16902271144234204No Hit
GTACTGCAATACCAGGTCGATGCGTGGAGTGGACGGAGCAAGCTCCTATTC419210.16591582181366601No Hit
GCCCCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATC414480.16404377239409434No Hit
TGGGTTAAGACTCAGAGTTGTTCCTCTCCACGGAAATCTTTAGTAAAAGGC413640.16371131541472012No Hit
GCCAGTCAAATTTAGCAGTGGGGGGTTGTATACCAACTTTAGTGACACTAA410850.16260708330465562No Hit
CCCGCTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCA398630.1577706258189969No Hit
CAAGTGCAGTAGTGAGAAGGGGGGAAAGAGTAGAACAAGGAGTTCGATCTG369730.14633252260004947No Hit
GTCCAAGGCATGAACCAGGCATGGGCACGGATCAAAAGCCAAGCTGGGATG367800.14556866311172528No Hit
GTGAGAAGGGGGGAAAGAGTAGAACAAGGAGTTCGATCTGTAACTGACTGT361280.14298816370039183No Hit
AGTCAAGTGAAGCAGTGGGAGTGGAGAAGGAACAAAGAAATCTGTAACTGG354910.14046703160680377No Hit
ACCAAGCGTCCCTGGCTGCTACAGGAGAATAGCAGACAGGTATAGTTAAGA346780.13724932298500298No Hit
CAGGTCGATGCGTGGAGTGGACGGAGCAAGCTCCTATTCCATCTCCTATTT346280.13705143192585165No Hit
ACCAGGTCGATGCGTGGAGTGGACGGAGCAAGCTCCTATTCCATCTCCTAT344840.13648150567549577No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGAC337720.1336635369931807No Hit
GTTTCAGACTCACACTCGTTCCTCTCCACGGAAATCTTTAGTAAAAGGCGA335620.1328323945447451No Hit
AGTCAAATTTAGCAGTGGGGGGTTGTATACCAACTTTAGTGACACTAATGT332710.13168066858048427No Hit
CAAAAAATTGGGTTAAGACTCAGAGTTGTTCCTCTCCACGGAAATCTTTAG332600.131637132547471No Hit
CACATTTGGGGAAATCGCAGGGGTCAGCACATCCGGAGTGCAATGGATAAG330000.13060809903988402No Hit
CCAGGCGGTCTCCCATCCAAGTACTAACCAGGCCCGACCCTGCTTAGCTTC329670.13047749094084415No Hit
CAGGCGGTCTCCCATCCAAGTACTAACCAGGCCCGACCCTGCTTAGCTTCC321090.12708168036580716No Hit
CAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTCAGCACATCCGGAG317740.1257558102694932No Hit
CATCTCCCTGCTCCAAAAATCCATTTAATATATTGTCCTCGGATAGAGGAC312280.12359483990356057No Hit
GGGGGAAAGAGTAGAACAAGGAGTTCGATCTGTAACTGACTGTGAACAATC300520.11894044219232106No Hit
CTCAGAGTACAAAGACTGATCCTTTGCCCAGGACCTTCCCGCCAGGCAGAC299230.11842988325971061No Hit
CCAGGTCGATGCGTGGAGTGGACGGAGCAAGCTCCTATTCCATCTCCCTGC296540.11736522936147639No Hit
GACCAAGCGTCCCTGGCTGCTACAGGAGAATAGCAGACAGGTATAGTTAAG295130.11680717657466962No Hit
GCCTCAAAAAATTGGGTTAAGACTCAGAGTTGTTCCTCTCCACGGAAATCT285170.11286518667637493No Hit
AGGTCGATGCGTGGAGTGGACGGAGCAAGCTCCTATTCCATCTCCCTGCTC278050.11004721799405985No Hit
ACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGG277070.10965935151812324No Hit
GATGCGTGGAGTGGACGGAGCAAGCTCCTATTCCATCTCCCTGCTCCAAAA269030.1064772632869697No Hit
GGAGGTACTGCAATACCAGGTCGATGCGTGGAGTGGACGGAGCAAGCTCCT268310.10619230016179178No Hit
CTCTAAATCGATCAATAAATAGGACACTTCTGCCAAAGGAACTCGTGCACA261790.10361180075045831No Hit
CTATTCCATCTCCCTGCTCCAAAAATCCATTTAATATATTGTCCTCGGATA260940.10327538594990103No Hit
GTCTAGAAGTGCCCATGACGTGAGCCCACTGGAGCCCTGCTCACTCCTCGG259670.10277274265965662No Hit
CTACCGCAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGG256920.10168434183432426No Hit
TCCTATTCCATCTCCCTGCTCCAAAAATCCATTTAATATATTGTCCTCGGA256260.10142312563624449No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)