FastQCFastQC Report
Wed 8 May 2024
EGAF00005204051

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005204051
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25266427
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCATTGCACTC14484695.73278129115763No Hit
GGGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCA9387553.715424424672313No Hit
CTTCAGATCGCATAAATCTTTCGCCTTTTACTAAAGATTTCCGTGGAGAG9354483.702335910020044No Hit
ACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCATTGCACT5893772.3326487753887797No Hit
GGGGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCC5456572.1596128332668485No Hit
AGGGGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATC5410632.1414306027520236No Hit
GGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCAT4543401.7981964762963913No Hit
ATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCATTGCACTCCG3958411.5666678949105073No Hit
CATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCATTGCACTCC3895171.5416386337490455No Hit
ATCTGTTCTTATCAGTTTAATATCTGATACGTCCTCTATCCGAGGACAAT3854231.525435313825734No Hit
CACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTCG3711371.4688938804050133No Hit
CGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCATTGCACTCCGGATGTGC3388221.3409968888755028No Hit
GGCTTATCCATTGCACTCCGGATGTGCTGACCCCTGCGATTTCCCCAAAT2819251.1158087370248275No Hit
CCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTC2618111.0362011217494267No Hit
CCCTGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTCGGG2540121.0053340743430008No Hit
TGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCATTGCACTCCGG2537011.0041031919550794No Hit
ACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGT2373670.9394561407515198No Hit
GATCAAGTGTAGTATCTGTTCTTATCAGTTTAATATCTGATACGTCCTCT2351100.9305233383414282No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTTTTTTTTTTTTTTTTTTT2315090.9162712242613489No Hit
CCTGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTCGGGC2196580.8693670854212984No Hit
ATCAAGTGTAGTATCTGTTCTTATCAGTTTAATATCTGATACGTCCTCTA2133100.8442428365514444No Hit
GTAGTGAGTTATCTCAATTGATTGTTCACAGTCAGTTACAGATCGAACTC1748260.6919300461438415No Hit
GTTTAATATCTGATACGTCCTCTATCCGAGGACAATATATTAAATGGATT1688630.6683295584294526No Hit
CTGTTCTTATCAGTTTAATATCTGATACGTCCTCTATCCGAGGACAATAT1649900.6530009169875899No Hit
GATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCATTGC1562130.6182631204641638No Hit
GTGAGTTATCTCAATTGATTGTTCACAGTCAGTTACAGATCGAACTCCTT1548890.6130229652178363No Hit
ATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCATTGCA1447500.5728946162431278No Hit
GTTCTTATCAGTTTAATATCTGATACGTCCTCTATCCGAGGACAATATAT1371050.5426370732988879No Hit
CAAGTGTAGTATCTGTTCTTATCAGTTTAATATCTGATACGTCCTCTATC1327010.525206828808838No Hit
GTCTGATCTCGGAAGCTAAGCAGGGTCGGGCCTGGTTAGTACTTGGATGG1273910.5041907983269657No Hit
AAGGTAGTGAGTTATCTCAATTGATTGTTCACAGTCAGTTACAGATCGAA1254990.496702600648679No Hit
CTAAGATCAAGTGTAGTATCTGTTCTTATCAGTTTAATATCTGATACGTC1211150.47935151258228953No Hit
CTCGTCTGATCTCGGAAGCTAAGCAGGGTCGGGCCTGGTTAGTACTTGGA1190970.4713646294349415No Hit
CAGGGGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTAT1168090.4623091345681762No Hit
CACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCATTGCACTCCGGATGT1167680.46214686389967213No Hit
CTACAATATAGCTGCTAAGTGCTGTGTTGTCGTTCCCCCTGCTTAAAATA1072110.424321966853485No Hit
GTGCAGTGGTGTTTACAACTAATTGATCACAACCAGTTACAGATTTCTTT1066680.42217286995110154No Hit
ATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCATTGCACTCCGGAT998790.3953032219395326No Hit
CTTATCCATTGCACTCCGGATGTGCTGACCCCTGCGATTTCCCCAAATGT985690.3901184761897675No Hit
CGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTCGGGCCTGGTTAGTACT946230.3745009138015439No Hit
GATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCATTGCACTCCGGA944440.37379246380978204No Hit
CCCAGGGCGAGGCTTATCCATTGCACTCCGGATGTGCTGACCCCTGCGAT925640.3663517599856917No Hit
CGAAGGTAGTGAGTTATCTCAATTGATTGTTCACAGTCAGTTACAGATCG902470.3571814883046186No Hit
TTCAGATCGTATAAATCTTTCGCCTTTTACTAAAGATTTCCGTGGAGAGG878200.34757585629341264No Hit
CTTCAGATCGTATAAATCTTTCGCCTTTTACTAAAGATTTCCGTGGAGAG821130.3249885708018787No Hit
AAGATCAAGTGTAGTATCTGTTCTTATCAGTTTAATATCTGATACGTCCT812060.32139882698887345No Hit
GAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCATT798360.3159766119681267No Hit
GGTGGTTTTCCCAGGGCGAGGCTTATCCATTGCACTCCGGATGTGCTGAC796890.3153948122542218No Hit
TACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCATTGCAC741360.293417031224874No Hit
AGTATCTGTTCTTATCAGTTTAATATCTGATACGTCCTCTATCCGAGGAC738000.292087203307377No Hit
GTGTAGTATCTGTTCTTATCAGTTTAATATCTGATACGTCCTCTATCCGA726010.2873417757089279No Hit
ATCTCGTCTGATCTCGGAAGCTAAGCAGGGTCGGGCCTGGTTAGTACTTG723380.28630086873779187No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTTTTTTTTTTTGTTTTTTT702070.2778667517967618No Hit
CTGATCTCGGAAGCTAAGCAGGGTCGGGCCTGGTTAGTACTTGGATGGGA684900.27107117282550475No Hit
ATCTGATACGTCCTCTATCCGAGGACAATATATTAAATGGATTTTTGGAG658540.26063835618704617No Hit
AGTGCAGTGGTGTTTACAACTAATTGATCACAACCAGTTACAGATTTCTT644090.25491930457757245No Hit
CCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCA641440.25387048196407036No Hit
AGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCATTG601710.2381460584039049No Hit
AGTGAGTTATCTCAATTGATTGTTCACAGTCAGTTACAGATCGAACTCCT596620.23613152742174426No Hit
CTGATACGTCCTCTATCCGAGGACAATATATTAAATGGATTTTTGGAGCA595320.2356170106679508No Hit
TTCAGATCGCATAAATCTTTCGCCTTTTACTAAAGATTTCCGTGGAGAGG587830.2326526026018637No Hit
GTTATCTCAATTGATTGTTCACAGTCAGTTACAGATCGAACTCCTTGTTC586210.2320114355702134No Hit
ACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTCGG578030.2287739378424975No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTTTTTTTTTTTTTTTTTTTT573260.2268860571381937No Hit
GTATCTGTTCTTATCAGTTTAATATCTGATACGTCCTCTATCCGAGGACA561700.2223108158506147No Hit
GCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTCGGGCCTGGTTAGT545390.21585560950109803No Hit
GTGGTTTTCCCAGGGCGAGGCTTATCCATTGCACTCCGGATGTGCTGACC537300.21265373216402939No Hit
GGCTAAGATCAAGTGTAGTATCTGTTCTTATCAGTTTAATATCTGATACG535870.21208776373485652No Hit
GGTAGTGAGTTATCTCAATTGATTGTTCACAGTCAGTTACAGATCGAACT525570.20801120791633892No Hit
CTCTTCAGATCGCATAAATCTTTCGCCTTTTACTAAAGATTTCCGTGGAG507320.20078818425731507No Hit
CTTCAAACCGTATAAATCTTTCGCCTTTTACTAAAGATTTCCGTGGAGAG505140.19992537923941522No Hit
GCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGC430060.17021005779725007No Hit
GATCTCGTCTGATCTCGGAAGCTAAGCAGGGTCGGGCCTGGTTAGTACTT429290.16990530556615702No Hit
CATAAATCTTTCGCCTTTTACTAAAGATTTCCGTGGAGAGGAACAACTCT426460.16878524217136046No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTTTTTTTTTTTTGTTTTTT421510.1668261206857622No Hit
ATGGTAGTGGGTTATCAGAACTTATTAACATTAGTGTCACTAAAGTTGGT420320.166355139964982No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGTTTTTTTTTTTTTTTTTTT412590.16329574419050227No Hit
AGATCAAGTGTAGTATCTGTTCTTATCAGTTTAATATCTGATACGTCCTC408880.16182739253159933No Hit
GCTAAGATCAAGTGTAGTATCTGTTCTTATCAGTTTAATATCTGATACGT401720.15899359256455217No Hit
AGGTAGTGAGTTATCTCAATTGATTGTTCACAGTCAGTTACAGATCGAAC401660.158969845637454No Hit
GGTGTTTACAACTAATTGATCACAACCAGTTACAGATTTCTTTGTTCCTT392330.15527719847369No Hit
CTGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTCGGGCC388730.15385238284780037No Hit
CCTCTATCCGAGGACAATATATTAAATGGATTTTTGGAGCAGGGAGATGG385740.15266899431407535No Hit
TACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGG383530.15179431583262643No Hit
CAGGGCGAGGCTTATCCATTGCACTCCGGATGTGCTGACCCCTGCGATTT363080.14370057151333665No Hit
CATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAG360400.14263987543628548No Hit
GAAGGTAGTGAGTTATCTCAATTGATTGTTCACAGTCAGTTACAGATCGA349330.13825856738667483No Hit
CGCATAAATCTTTCGCCTTTTACTAAAGATTTCCGTGGAGAGGAACAACT331660.13126509735626649No Hit
GGTTTTCCCAGGGCGAGGCTTATCCATTGCACTCCGGATGTGCTGACCCC315430.124841553576214No Hit
CGGCCTTTTGGCTAAGATCAAGTGTAGTATCTGTTCTTATCAGTTTAATA301310.11925311006578017No Hit
GTGGGTTATCAGAACTTATTAACATTAGTGTCACTAAAGTTGGTATACAA298450.11812117320743451No Hit
GTAGTATCTGTTCTTATCAGTTTAATATCTGATACGTCCTCTATCCGAGG294900.11671614668746001No Hit
TGTTCTTATCAGTTTAATATCTGATACGTCCTCTATCCGAGGACAATATA293700.11624120814549678No Hit
ATAAATCTTTCGCCTTTTACTAAAGATTTCCGTGGAGAGGAACAACTCTG289760.11468182659938424No Hit
GTTTTCCCAGGGCGAGGCTTATCCATTGCACTCCGGATGTGCTGACCCCT285880.11314619198036983No Hit
TCTGTTCTTATCAGTTTAATATCTGATACGTCCTCTATCCGAGGACAATA278210.11011054313298828No Hit
AAATCTTTCGCCTTTTACTAAAGATTTCCGTGGAGAGGAACAACTCTGAG277620.10987703168318971No Hit
TAGTGAGTTATCTCAATTGATTGTTCACAGTCAGTTACAGATCGAACTCC275540.10905380487712013No Hit
GCTTATCCATTGCACTCCGGATGTGCTGACCCCTGCGATTTCCCCAAATG273410.10821078896513545No Hit
CGATGGTAGTGGGTTATCAGAACTTATTAACATTAGTGTCACTAAAGTTG272820.10797727751533685No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)