FastQCFastQC Report
Wed 8 May 2024
EGAF00005204052

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005204052
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35296130
Total Bases1.8 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCG21140165.989370506058313No Hit
CCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTC18608195.2720199069983025No Hit
CCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGC12066573.4186665790272195No Hit
ACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGT10407952.9487510387116096No Hit
GTCCCCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAA7895362.236891126590932No Hit
CTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGG7646122.16627715276434No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTACTCCATCTCGTAT7072862.0038627464257415TruSeq Adapter, Index 14 (97% over 38bp)
CCCCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATC4900801.388480833451146No Hit
CCCACATTTGGGGAAATCGCAGGGGTCAGCACATCCGGAGTGCAATGGAT4818911.3652799896192587No Hit
CACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTCA3674561.0410659752216462No Hit
CTCCTATTCCATCTCCCTGCTCCAAAAATCCATTTAATATATTGTCCTCG3390020.960450904957569No Hit
GGTCGATGCGTGGAGTGGACGGAGCAAGCTCCTATTCCATCTCCCTGCTC3173710.8991665658529703No Hit
GTCGATGCGTGGAGTGGACGGAGCAAGCTCCTATTCCATCTCCTATTTCC3112690.8818785515579186No Hit
GGTCGATGCGTGGAGTGGACGGAGCAAGCTCCTATTCCATCTCCTATTTC2950610.8359585030993483No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA2942370.8336239695400033No Hit
CCACATTTGGGGAAATCGCAGGGGTCAGCACATCCGGAGTGCAATGGATA2778770.787273278968544No Hit
GTCGATGCGTGGAGTGGACGGAGCAAGCTCCTATTCCATCTCCCTGCTCC2603800.7377012720658044No Hit
GTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTCAGCACATCCGGAGT2508180.7106104833589405No Hit
CCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCA2417430.6848994493163981No Hit
CTCCTATTTCCAAAAATCCATTTAATATATTGTCCTCGGATAGAGGACGT2316460.6562929136990372No Hit
GTGCAGTAGTGAGAAGGGGGGAAAGAGTAGAACAAGGAGTTCGATCTGTA2202060.6238814283605596No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA2192530.6211814156396183No Hit
CCTGGAAGTACTGCAATACCAGGTCGATGCGTGGAGTGGACGGAGCAAGC2121280.6009950665979529No Hit
GTCAAGTGCAGTAGTGAGAAGGGGGGAAAGAGTAGAACAAGGAGTTCGAT2056720.5827041094873574No Hit
GTCAAAAATTGCCAATGCCGACTATATTTCAAGTCGTCATGGCGGGGTAT1939690.5495474999667102No Hit
CACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAG1889470.5353193112106058No Hit
CCCCCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAAT1827780.5178414744052676No Hit
GTTAAGACTCAGAGTTGTTCCTCTCCACGGAAATCTTTAGTAAAAGGCGA1779290.5041034243697539No Hit
GCCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGT1634590.46310742849145214No Hit
CTGCAATACCAGGTCGATGCGTGGAGTGGACGGAGCAAGCTCCTATTCCA1526770.43256017019429605No Hit
ATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTCAGCACATCC1513170.4287070565526589No Hit
CTCCTATTCCATCTCCTATTTCCAAAAATCCATTTAATATATTGTCCTCG1504180.4261600351086649No Hit
ACCAGGTCGATGCGTGGAGTGGACGGAGCAAGCTCCTATTCCATCTCCCT1480520.4194567506409343No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT1465410.4151758280582035No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACG1436110.40687463469791163No Hit
CAGGTCGATGCGTGGAGTGGACGGAGCAAGCTCCTATTCCATCTCCCTGC1375660.3897481111951933No Hit
CAGGTCGATGCGTGGAGTGGACGGAGCAAGCTCCTATTCCATCTCCTATT1314020.37228444024883184No Hit
ACCAGGTCGATGCGTGGAGTGGACGGAGCAAGCTCCTATTCCATCTCCTA1274570.36110757751628864No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT1265150.35843872968509577No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG1213220.3437260685519914No Hit
CCTGGAGGTACTGCAATACCAGGTCGATGCGTGGAGTGGACGGAGCAAGC1200970.3402554331027226No Hit
GGCTAGTCAAGTGAAGCAGTGGGAGTGGAGAAGGAACAAAGAAATCTGTA1189980.3371417772996643No Hit
ATTCCATCTCCCTGCTCCAAAAATCCATTTAATATATTGTCCTCGGATAG1133550.3211541888586652No Hit
ATTGGGTTAAGACTCAGAGTTGTTCCTCTCCACGGAAATCTTTAGTAAAA1098770.3113004173545372No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT1058800.29997622968863724No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA1052560.29820833048835665No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTACTCCATCGCGTAT1034950.29321911495679553TruSeq Adapter, Index 14 (97% over 38bp)
GCAGTAGTGAGAAGGGGGGAAAGAGTAGAACAAGGAGTTCGATCTGTAAC1016020.287855920748252No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAAC951850.2696754573376742No Hit
CCCCGCTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCG938630.26593000422425916No Hit
GCCCCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAAT926060.2623687072775401No Hit
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC898730.25462564876092647No Hit
GCTCCTATTCCATCTCCCTGCTCCAAAAATCCATTTAATATATTGTCCTC897700.25433383206600835No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT889590.25203612974000267No Hit
GCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT881320.2496930966652718No Hit
CTCCCATCCAAGTACTAACCAGGCCCGACCCTGCTTAGCTTCCGAGATCA834900.23654151319138952No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG832560.23587855099128432No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG820830.2325552404753722No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA779000.22070408285554252No Hit
CCGCAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTC769650.21805506722691695No Hit
CGATGCGTGGAGTGGACGGAGCAAGCTCCTATTCCATCTCCCTGCTCCAA754270.2136976490057125No Hit
CGATGCGTGGAGTGGACGGAGCAAGCTCCTATTCCATCTCCTATTTCCAA752200.21311118244408098No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC711540.2015915059243039No Hit
GTCAAATTTAGCAGTGGGGGGTTGTATACCAACTTTAGTGACACTAATGT645070.18275941300080206No Hit
GTCAAGTGAAGCAGTGGGAGTGGAGAAGGAACAAAGAAATCTGTAACTGG642410.18200578930324657No Hit
GTACTGCAATACCAGGTCGATGCGTGGAGTGGACGGAGCAAGCTCCTATT632290.17913861944638124No Hit
GGGAAAAGTTTTCAATTAGCAATAATCGCGCCTCGGATAAACCTCATTGG625680.17726589288967373No Hit
ACCGCAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGT620060.17567365034070306No Hit
CCCGCTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGC609200.17259682577098395No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA605410.17152305366055712No Hit
GCTCCTATTCCATCTCCTATTTCCAAAAATCCATTTAATATATTGTCCTC602830.1707920953373642No Hit
GCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT597900.16939534164227069No Hit
CTGGAAGTACTGCAATACCAGGTCGATGCGTGGAGTGGACGGAGCAAGCT589440.16699847830342873No Hit
CTAGTCAAGTGAAGCAGTGGGAGTGGAGAAGGAACAAAGAAATCTGTAAC574640.16280538404635295No Hit
CACCACTCATCTTTGTGAGATGGGTCTCAGAGGGCCCCGTCCCTGCTGAC548580.1554221383477452No Hit
GGGGTATTGGGAAAAGTTTTCAATTAGCAATAATCGCGCCTCGGATAAAC544250.154195374960371No Hit
CCACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAA528960.14986345528532447No Hit
GCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTCAGCACATCCGG527790.14953197418527187No Hit
CTGGAGGTACTGCAATACCAGGTCGATGCGTGGAGTGGACGGAGCAAGCT526660.14921182577240055No Hit
GGTTAAGACTCAGAGTTGTTCCTCTCCACGGAAATCTTTAGTAAAAGGCG515210.14596784406675745No Hit
CTAGTCAAGTGCAGTAGTGAGAAGGGGGGAAAGAGTAGAACAAGGAGTTC514410.14574119032313174No Hit
GATGCGTGGAGTGGACGGAGCAAGCTCCTATTCCATCTCCCTGCTCCAAA507590.14380896715872252No Hit
CCATCTCCCTGCTCCAAAAATCCATTTAATATATTGTCCTCGGATAGAGG503070.14252837350723718No Hit
GGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACC501010.14194474011740096No Hit
CAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTCAGCACATCCGGA474190.1343461733623488No Hit
GCCAGTCAAATTTAGCAGTGGGGGGTTGTATACCAACTTTAGTGACACTA473080.1340316912930681No Hit
CCAGGTCGATGCGTGGAGTGGACGGAGCAAGCTCCTATTCCATCTCCCTG467260.132382785308191No Hit
TCCCCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAAT466520.13217313059533722No Hit
ACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGG465090.13176798702860626No Hit
CTCTCTTCCTCGTGGTTTTCGGTGCTCTACACGTTCAGAGAAACTTCTCT456850.12943345346926136No Hit
TACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGG455870.12915580263331985No Hit
GGAGGTACTGCAATACCAGGTCGATGCGTGGAGTGGACGGAGCAAGCTCC434260.12303331838363016No Hit
AGACTGTCAAAAATTGCCAATGCCGACTATATTTCAAGTCGTCATGGCGG433320.12276700023486994No Hit
GGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAA429310.12163089834494603No Hit
CCTATTTCCAAAAATCCATTTAATATATTGTCCTCGGATAGAGGACGTAT427110.12100760054997531No Hit
GCCTACAGCACCCGGTATTCCCAGGCGGTCTCCCATCCAAGTACTAACCA425430.12053162768836129No Hit
AGCCAGTCAAATTTAGCAGTGGGGGGTTGTATACCAACTTTAGTGACACT421670.11946635509332043No Hit
CTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC412810.1169561648826656No Hit
CCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC408750.1158058971337651No Hit
GTCAAAAATTGCCAGTGCCGACTATATTGCAAGTCGTCACGGCGGGGTAT408370.11569823660554288No Hit
AGGTCGATGCGTGGAGTGGACGGAGCAAGCTCCTATTCCATCTCCCTGCT403730.11438364489251371No Hit
GATGCGTGGAGTGGACGGAGCAAGCTCCTATTCCATCTCCTATTTCCAAA401360.11371218317702253No Hit
CTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGGTGCTCTACAC392500.11120199296636771No Hit
GTGAGAAGGGGGGAAAGAGTAGAACAAGGAGTTCGATCTGTAACTGACTG391710.1109781723945373No Hit
GTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACT391200.11083368063297591No Hit
GGGGTCTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCG390640.11067502301243791No Hit
CATCTCCCTGCTCCAAAAATCCATTTAATATATTGTCCTCGGATAGAGGA379040.107388543729865No Hit
CTACCGCAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGG378820.10732621395036794No Hit
TCCTATTCCATCTCCCTGCTCCAAAAATCCATTTAATATATTGTCCTCGG373520.10582463289884754No Hit
CTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC371310.1051985019320815No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG369240.10461203537044997No Hit
GGGTCGATGCGTGGAGTGGACGGAGCAAGCTCCTATTCCATCTCCCTGCT363300.10292913132402902No Hit
GACCAAGCGTCCCTGGCTGCTACAGGAGAATAGCAGACAGGTATAGTTAA363090.10286963471632725No Hit
AGGTCGATGCGTGGAGTGGACGGAGCAAGCTCCTATTCCATCTCCTATTT358760.10164287132895305No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)