FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005204052

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005204052
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35296130
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGC21137195.988529054035102No Hit
CCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTCA18601765.270198177533911No Hit
CCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCA12058633.416417040621734No Hit
ACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTC10406562.94835722783206No Hit
GTCCCCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAAT7886912.2344970964238855No Hit
CTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGG7638332.1640701119357844No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTACTCCATCTCGTATG7071872.0035822624180044TruSeq Adapter, Index 14 (97% over 38bp)
CCCCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCG4899681.38816351821007No Hit
CCCACATTTGGGGAAATCGCAGGGGTCAGCACATCCGGAGTGCAATGGATA4817931.365002338783317No Hit
CACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTCAG3671771.0402755202907514No Hit
CTCCTATTCCATCTCCCTGCTCCAAAAATCCATTTAATATATTGTCCTCGG3387210.9596547836830835No Hit
GGTCGATGCGTGGAGTGGACGGAGCAAGCTCCTATTCCATCTCCCTGCTCC3173290.8990475726375667No Hit
GTCGATGCGTGGAGTGGACGGAGCAAGCTCCTATTCCATCTCCTATTTCCA3111940.8816660636732695No Hit
GGTCGATGCGTGGAGTGGACGGAGCAAGCTCCTATTCCATCTCCTATTTCC2950070.835805511822401No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT2938750.8325983613500971No Hit
CCACATTTGGGGAAATCGCAGGGGTCAGCACATCCGGAGTGCAATGGATAA2775790.7864289937735384No Hit
GTCGATGCGTGGAGTGGACGGAGCAAGCTCCTATTCCATCTCCCTGCTCCA2600730.7368314883246406No Hit
GTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTCAGCACATCCGGAGTG2507740.7104858237999463No Hit
CCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAG2416770.6847124599779069No Hit
CTCCTATTTCCAAAAATCCATTTAATATATTGTCCTCGGATAGAGGACGTA2315900.6561342560784993No Hit
GTGCAGTAGTGAGAAGGGGGGAAAGAGTAGAACAAGGAGTTCGATCTGTAA2200740.6235074496835772No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGAC2192260.6211049200011446No Hit
CCTGGAAGTACTGCAATACCAGGTCGATGCGTGGAGTGGACGGAGCAAGCT2116390.5996096455900406No Hit
GTCAAGTGCAGTAGTGAGAAGGGGGGAAAGAGTAGAACAAGGAGTTCGATC2054340.5820298146000709No Hit
GTCAAAAATTGCCAATGCCGACTATATTTCAAGTCGTCATGGCGGGGTATT1928200.5462921855738859No Hit
CACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGG1888800.5351294887003193No Hit
CCCCCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATC1827650.5178046431719285No Hit
GTTAAGACTCAGAGTTGTTCCTCTCCACGGAAATCTTTAGTAAAAGGCGAA1778450.5038654379389469No Hit
GCCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTC1634390.46305076505554577No Hit
CTGCAATACCAGGTCGATGCGTGGAGTGGACGGAGCAAGCTCCTATTCCAT1524310.431863209932647No Hit
ATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTCAGCACATCCG1512380.42848323598082855No Hit
CTCCTATTCCATCTCCTATTTCCAAAAATCCATTTAATATATTGTCCTCGG1502920.42580305546245434No Hit
ACCAGGTCGATGCGTGGAGTGGACGGAGCAAGCTCCTATTCCATCTCCCTG1480220.4193717554870746No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT1464540.4149293421120106No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA1434830.40651198870811045No Hit
CAGGTCGATGCGTGGAGTGGACGGAGCAAGCTCCTATTCCATCTCCCTGCT1369530.3880113768846613No Hit
CAGGTCGATGCGTGGAGTGGACGGAGCAAGCTCCTATTCCATCTCCTATTT1310220.37120783496660964No Hit
ACCAGGTCGATGCGTGGAGTGGACGGAGCAAGCTCCTATTCCATCTCCTAT1273050.36067693540339973No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG1264780.3583339023286689No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC1212800.3436070753365879No Hit
CCTGGAGGTACTGCAATACCAGGTCGATGCGTGGAGTGGACGGAGCAAGCT1198080.33943664645387467No Hit
GGCTAGTCAAGTGAAGCAGTGGGAGTGGAGAAGGAACAAAGAAATCTGTAA1187760.33651281316110293No Hit
ATTCCATCTCCCTGCTCCAAAAATCCATTTAATATATTGTCCTCGGATAGA1131950.3207008813714138No Hit
ATTGGGTTAAGACTCAGAGTTGTTCCTCTCCACGGAAATCTTTAGTAAAAG1098660.3112692524647886No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTC1058680.2999422316270934No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGAT1052160.2980950036165438No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTACTCCATCGCGTATG1034660.2931369529747312TruSeq Adapter, Index 14 (97% over 38bp)
GCAGTAGTGAGAAGGGGGGAAAGAGTAGAACAAGGAGTTCGATCTGTAACT1011950.28670281982755613No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACG951600.2696046280427911No Hit
CCCCGCTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGC938520.26589883933451064No Hit
GCCCCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATC926030.2623602077621541No Hit
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA898480.2545548194660434No Hit
GCTCCTATTCCATCTCCCTGCTCCAAAAATCCATTTAATATATTGTCCTCG897480.2542715022865113No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTG888800.25181230916817227No Hit
GCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC881210.24966193177552326No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA832310.2358077216964013No Hit
CTCCCATCCAAGTACTAACCAGGCCCGACCCTGCTTAGCTTCCGAGATCAG830370.23525808636810894No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT820020.2323257535599512No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG778910.2206785843093846No Hit
CCGCAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTCA769350.2179700720730573No Hit
CGATGCGTGGAGTGGACGGAGCAAGCTCCTATTCCATCTCCCTGCTCCAAA754150.21366365094416867No Hit
CGATGCGTGGAGTGGACGGAGCAAGCTCCTATTCCATCTCCTATTTCCAAA751940.2130375199774026No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCT710550.20131102191656708No Hit
GTCAAATTTAGCAGTGGGGGGTTGTATACCAACTTTAGTGACACTAATGTT644650.18264041978539855No Hit
GTCAAGTGAAGCAGTGGGAGTGGAGAAGGAACAAAGAAATCTGTAACTGGT642160.1819349600083635No Hit
GTACTGCAATACCAGGTCGATGCGTGGAGTGGACGGAGCAAGCTCCTATTC631260.17884680275146314No Hit
GGGAAAAGTTTTCAATTAGCAATAATCGCGCCTCGGATAAACCTCATTGGC625640.17725456020249247No Hit
ACCGCAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTC619980.1756509849663405No Hit
CCCGCTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCA608900.17251183061712433No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCAC605320.17149755511439924No Hit
GCTCCTATTCCATCTCCTATTTCCAAAAATCCATTTAATATATTGTCCTCG602680.17074959776043436No Hit
GCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC597800.1693670099243175No Hit
CTGGAAGTACTGCAATACCAGGTCGATGCGTGGAGTGGACGGAGCAAGCTC589400.16698714561624745No Hit
CTAGTCAAGTGAAGCAGTGGGAGTGGAGAAGGAACAAAGAAATCTGTAACT574350.16272322206428863No Hit
CACCACTCATCTTTGTGAGATGGGTCTCAGAGGGCCCCGTCCCTGCTGACA548390.1553683080836341No Hit
GGGGTATTGGGAAAAGTTTTCAATTAGCAATAATCGCGCCTCGGATAAACC541520.15342191906024824No Hit
CCACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAAC528870.1498379567391666No Hit
GCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTCAGCACATCCGGA527540.14946114489038884No Hit
CTGGAGGTACTGCAATACCAGGTCGATGCGTGGAGTGGACGGAGCAAGCTC526610.14919765991342393No Hit
GGTTAAGACTCAGAGTTGTTCCTCTCCACGGAAATCTTTAGTAAAAGGCGA514980.14590268111546506No Hit
CTAGTCAAGTGCAGTAGTGAGAAGGGGGGAAAGAGTAGAACAAGGAGTTCG514230.14569019323081595No Hit
GATGCGTGGAGTGGACGGAGCAAGCTCCTATTCCATCTCCCTGCTCCAAAA507480.14377780226897396No Hit
CCATCTCCCTGCTCCAAAAATCCATTTAATATATTGTCCTCGGATAGAGGA502840.1424632105559448No Hit
GGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCT500740.1418682444789273No Hit
CAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTCAGCACATCCGGAG474160.13433767384696282No Hit
GCCAGTCAAATTTAGCAGTGGGGGGTTGTATACCAACTTTAGTGACACTAA472790.1339495293110038No Hit
CCAGGTCGATGCGTGGAGTGGACGGAGCAAGCTCCTATTCCATCTCCCTGC467180.13236011993382843No Hit
TCCCCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATC466510.13217029742354192No Hit
ACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGG465030.1317509879978343No Hit
CTCTCTTCCTCGTGGTTTTCGGTGCTCTACACGTTCAGAGAAACTTCTCTA456740.1294022885795128No Hit
TACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGT455210.12896881329482865No Hit
GGAGGTACTGCAATACCAGGTCGATGCGTGGAGTGGACGGAGCAAGCTCCT433930.12293982371438456No Hit
AGACTGTCAAAAATTGCCAATGCCGACTATATTTCAAGTCGTCATGGCGGG433270.12275283437589334No Hit
GGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAAT428960.12153173733210978No Hit
CCTATTTCCAAAAATCCATTTAATATATTGTCCTCGGATAGAGGACGTATC427050.12099060151920338No Hit
GCCTACAGCACCCGGTATTCCCAGGCGGTCTCCCATCCAAGTACTAACCAG425400.12052312817297534No Hit
AGCCAGTCAAATTTAGCAGTGGGGGGTTGTATACCAACTTTAGTGACACTA421590.11944368971895786No Hit
CTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCC412780.11694766536727964No Hit
CCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA408600.11576339955683528No Hit
GTCAAAAATTGCCAGTGCCGACTATATTGCAAGTCGTCACGGCGGGGTATT405840.11498144414132654No Hit
AGGTCGATGCGTGGAGTGGACGGAGCAAGCTCCTATTCCATCTCCCTGCTC403660.11436381268994647No Hit
GATGCGTGGAGTGGACGGAGCAAGCTCCTATTCCATCTCCTATTTCCAAAA401250.11368101828727398No Hit
CTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGGTGCTCTACACG392390.11117082807661917No Hit
GTGAGAAGGGGGGAAAGAGTAGAACAAGGAGTTCGATCTGTAACTGACTGT391540.11093000847401684No Hit
GTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTG391180.11082801428938527No Hit
GGGGTCTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGC390630.11067218984064259No Hit
CATCTCCCTGCTCCAAAAATCCATTTAATATATTGTCCTCGGATAGAGGAC379020.10738287738627436No Hit
CTACCGCAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGG378740.10730354857600537No Hit
TCCTATTCCATCTCCCTGCTCCAAAAATCCATTTAATATATTGTCCTCGGA373320.10576796946294112No Hit
CTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCC371290.10519283558849087No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGC369160.1045893699960874No Hit
GGGTCGATGCGTGGAGTGGACGGAGCAAGCTCCTATTCCATCTCCCTGCTC363290.1029262981522337No Hit
GACCAAGCGTCCCTGGCTGCTACAGGAGAATAGCAGACAGGTATAGTTAAG363050.10285830202914598No Hit
AGGTCGATGCGTGGAGTGGACGGAGCAAGCTCCTATTCCATCTCCTATTTC358730.1016343718135671No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)