FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005204053

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005204053
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35296130
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCATTGCACTCC18949575.3687387257469865No Hit
GGGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCAT18665035.288123655482909No Hit
GGGGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCA10800963.0600975234395382No Hit
ACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCATTGCACTC8177582.3168489009984947No Hit
GGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCATT8074332.2875964022118005No Hit
AGGGGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCC7844042.2223512889373422No Hit
ATCTGTTCTTATCAGTTTAATATCTGATACGTCCTCTATCCGAGGACAATA6151421.7428029645176397No Hit
ATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCATTGCACTCCGG5315411.5059469692569696No Hit
CATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCATTGCACTCCG4967931.4074999157131391No Hit
CGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCATTGCACTCCGGATGTGCT4286341.214393759315823No Hit
GATCAAGTGTAGTATCTGTTCTTATCAGTTTAATATCTGATACGTCCTCTA4201641.19039679420945No Hit
CTTCAGATCGCATAAATCTTTCGCCTTTTACTAAAGATTTCCGTGGAGAGG4122321.1679240755289602No Hit
TGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCATTGCACTCCGGA3897501.1042287072265429No Hit
GGCTTATCCATTGCACTCCGGATGTGCTGACCCCTGCGATTTCCCCAAATG3776461.0699359958159718No Hit
CTAAGATCAAGTGTAGTATCTGTTCTTATCAGTTTAATATCTGATACGTCC3752271.0630825532430892No Hit
ATCAAGTGTAGTATCTGTTCTTATCAGTTTAATATCTGATACGTCCTCTAT3380770.9578302210468967No Hit
GTTTAATATCTGATACGTCCTCTATCCGAGGACAATATATTAAATGGATTT2901960.8221751223151095No Hit
GATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCATTGCA2737540.7755921116564337No Hit
CTGTTCTTATCAGTTTAATATCTGATACGTCCTCTATCCGAGGACAATATA2507910.7105339877204668No Hit
GTTCTTATCAGTTTAATATCTGATACGTCCTCTATCCGAGGACAATATATT2344370.6642002961797795No Hit
CAAGTGTAGTATCTGTTCTTATCAGTTTAATATCTGATACGTCCTCTATCC2253040.6383249381731084No Hit
ATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCATTGCAC2033840.5762218124196619No Hit
CAGGGGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATC1883140.5335259134641673No Hit
GTAGTGAGTTATCTCAATTGATTGTTCACAGTCAGTTACAGATCGAACTCC1769540.5013410818693155No Hit
AAGATCAAGTGTAGTATCTGTTCTTATCAGTTTAATATCTGATACGTCCTC1766070.5003579712563389No Hit
GATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCATTGCACTCCGGAT1583070.44851092740195597No Hit
CACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCATTGCACTCCGGATGTG1467180.4156772994659754No Hit
GAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCATTG1402650.397394841870766No Hit
GTGAGTTATCTCAATTGATTGTTCACAGTCAGTTACAGATCGAACTCCTTG1379220.39075672035432785No Hit
GTGTAGTATCTGTTCTTATCAGTTTAATATCTGATACGTCCTCTATCCGAG1360570.38547285495605327No Hit
AAGGTAGTGAGTTATCTCAATTGATTGTTCACAGTCAGTTACAGATCGAAC1252770.3549312630024878No Hit
GGCTAAGATCAAGTGTAGTATCTGTTCTTATCAGTTTAATATCTGATACGT1241030.3516051193147804No Hit
AGTATCTGTTCTTATCAGTTTAATATCTGATACGTCCTCTATCCGAGGACA1227590.34779733642186833No Hit
GGTGGTTTTCCCAGGGCGAGGCTTATCCATTGCACTCCGGATGTGCTGACC1166800.3305744850781091No Hit
TACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCATTGCACT1165810.33029400107037227No Hit
CTTATCCATTGCACTCCGGATGTGCTGACCCCTGCGATTTCCCCAAATGTG1150790.32603857703379946No Hit
GTGCAGTGGTGTTTACAACTAATTGATCACAACCAGTTACAGATTTCTTTG1143020.3238372025488347No Hit
ATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCATTGCACTCCGGATG1128860.31982543128665947No Hit
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGA1086390.3077929506719292No Hit
CCCAGGGCGAGGCTTATCCATTGCACTCCGGATGTGCTGACCCCTGCGATT1038600.29425322266208787No Hit
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGA1035870.29347976676196513No Hit
CTACAATATAGCTGCTAAGTGCTGTGTTGTCGTTCCCCCTGCTTAAAATAA1032320.292473990774626No Hit
GTATCTGTTCTTATCAGTTTAATATCTGATACGTCCTCTATCCGAGGACAA1015920.2878275890302988No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG939020.26604049792427664No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGAT927160.2626803561750254No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGAT835450.2366973376401322No Hit
GCTAAGATCAAGTGTAGTATCTGTTCTTATCAGTTTAATATCTGATACGTC833570.2361647013426118No Hit
GTGGCAGTATCGTAGCCAATGAGGTTTATCCGAGGCGCGATTATTGCTAAT832470.23585305244512642No Hit
GTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGC813550.2304926914083782No Hit
CAGTATCGTAGCCAATGAGGTTTATCCGAGGCGCGATTATTGCTAATTGAA800570.22681523441805096No Hit
AGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCATTGC789990.2238177386586008No Hit
CGAAGGTAGTGAGTTATCTCAATTGATTGTTCACAGTCAGTTACAGATCGA787230.22303578324309206No Hit
ATCTGATACGTCCTCTATCCGAGGACAATATATTAAATGGATTTTTGGAGC783950.22210650289422665No Hit
GATCATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCA763990.216451491990765No Hit
GTGGTTTTCCCAGGGCGAGGCTTATCCATTGCACTCCGGATGTGCTGACCC750370.21259271200553714No Hit
CCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTC707540.20045823720617528No Hit
GCAGTATCGTAGCCAATGAGGTTTATCCGAGGCGCGATTATTGCTAATTGA702550.19904448448030987No Hit
CTGATACGTCCTCTATCCGAGGACAATATATTAAATGGATTTTTGGAGCAG691570.19593366184904693No Hit
CATCAATATGGTGACCTCCCGGGAGCGGGGGACCACCAGGTTGCCTAAGGA686440.194480244718047No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA681970.1932138169255383No Hit
ATCTGATACGTCCTCTATCCGAGGACAATATATTAAATGGATTTTTGGAAA677140.19184539494839803No Hit
CTGATACGTCCTCTATCCGAGGACAATATATTAAATGGATTTTTGGAAATA676640.19170373635863197No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG667730.18917938028900053No Hit
GGATCATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGC664870.18836909315553857No Hit
CCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTTC643010.18217577961096584No Hit
TCTGTTCTTATCAGTTTAATATCTGATACGTCCTCTATCCGAGGACAATAT636950.18045887750300105No Hit
AGATCAAGTGTAGTATCTGTTCTTATCAGTTTAATATCTGATACGTCCTCT628530.17807334685134038No Hit
CACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAACGGAGCAG625200.1771299006434983No Hit
CGTAGCCAATGAGGTTTATCCGAGGCGCGATTATTGCTAATTGAAAACTTT614700.17415507025841076No Hit
AGTGCAGTGGTGTTTACAACTAATTGATCACAACCAGTTACAGATTTCTTT605500.17154855220671503No Hit
GGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAACG596040.1688683716883409No Hit
TTCAGATCGTATAAATCTTTCGCCTTTTACTAAAGATTTCCGTGGAGAGGA589730.16708064028549305No Hit
GGTAGTGAGTTATCTCAATTGATTGTTCACAGTCAGTTACAGATCGAACTC588210.1666499981726042No Hit
ATCGTAGCCAATGAGGTTTATCCGAGGCGCGATTATTGCTAATTGAAAACT582230.16495576143900195No Hit
GTAGTATCTGTTCTTATCAGTTTAATATCTGATACGTCCTCTATCCGAGGA579550.16419647139785581No Hit
GGGATCATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAG577780.16369499999008388No Hit
AGTGAGTTATCTCAATTGATTGTTCACAGTCAGTTACAGATCGAACTCCTT554910.1572155360941837No Hit
CGGCCTTTTGGCTAAGATCAAGTGTAGTATCTGTTCTTATCAGTTTAATAT540660.1531782662858506No Hit
ATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGA538350.15252380360113135No Hit
CGGGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAA527550.14946397806218414No Hit
CCTCTATCCGAGGACAATATATTAAATGGATTTTTGGAAATAGGAGATGGA522100.14791989943373396No Hit
GTTATCTCAATTGATTGTTCACAGTCAGTTACAGATCGAACTCCTTGTTCT501230.14200706989689804No Hit
GGTTTTCCCAGGGCGAGGCTTATCCATTGCACTCCGGATGTGCTGACCCCT492120.1394260503913602No Hit
GATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGT484030.1371340144089451No Hit
GGGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAAC458690.12995475707960052No Hit
AGGTAGTGAGTTATCTCAATTGATTGTTCACAGTCAGTTACAGATCGAACT457660.1296629403846824No Hit
GGCAGTATCGTAGCCAATGAGGTTTATCCGAGGCGCGATTATTGCTAATTG456680.1293852895487409No Hit
CTTCAGATCGTATAAATCTTTCGCCTTTTACTAAAGATTTCCGTGGAGAGG455830.12914446994613857No Hit
GAAGGTAGTGAGTTATCTCAATTGATTGTTCACAGTCAGTTACAGATCGAA449980.12748706444587551No Hit
CAGGGCGAGGCTTATCCATTGCACTCCGGATGTGCTGACCCCTGCGATTTC447720.12684676762013286No Hit
CTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCG447000.12664277925086972No Hit
TGTTCTTATCAGTTTAATATCTGATACGTCCTCTATCCGAGGACAATATAT444650.1259769838789692No Hit
CCTCTATCCGAGGACAATATATTAAATGGATTTTTGGAGCAGGGAGATGGA441310.1250307044993318No Hit
GTTTTCCCAGGGCGAGGCTTATCCATTGCACTCCGGATGTGCTGACCCCTG438740.12430257934793418No Hit
CTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATC435760.12345829415292837No Hit
GGTGTTTACAACTAATTGATCACAACCAGTTACAGATTTCTTTGTTCCTTC430040.1218377198860045No Hit
ATGGTAGTGGGTTATCAGAACTTATTAACATTAGTGTCACTAAAGTTGGTA426780.1209141058807297No Hit
GTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATC425200.1204664647370689No Hit
GTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATC421190.11933036284714499No Hit
CATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATCTGGCTGGCT407970.11558490973373No Hit
CTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTA398880.11300955657178281No Hit
CCTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTA394550.1117827931844086No Hit
TGGCAGTATCGTAGCCAATGAGGTTTATCCGAGGCGCGATTATTGCTAATT391860.11102066997146713No Hit
GGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTC383580.10867480372494095No Hit
CAGGGATCATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAG375990.10652442633229196No Hit
CAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGT373970.105952125629637No Hit
GGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGAT367550.10413322933704064No Hit
CTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAAC363740.10305379088302316No Hit
AAGTGTAGTATCTGTTCTTATCAGTTTAATATCTGATACGTCCTCTATCCG361210.10233699841880682No Hit
TATCTGTTCTTATCAGTTTAATATCTGATACGTCCTCTATCCGAGGACAAT356630.10103940573654958No Hit
GATCGCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGT353350.10011012538768416No Hit
CCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACCAC353100.10003929609280111No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCAGA546300.040.0276451
CGGCCTT65950.039.7680131
AGATCGC512200.038.0306785
TCGCATA517950.037.6267058
GATCGCA519600.037.502046
ATCGCAT522350.037.287387
CGCATAA533050.036.5911069
CAGGGGA242000.036.1532821
CGGTGGC304600.035.9946481
GCATAAA544950.035.81684510
CATAAAT562950.034.7235811
CCGTATA16950.033.051838
GGCGATC34550.032.76622
TTCAGAT673400.032.4360962
CTACAAT146350.032.3620221
TCAGATC684400.031.8379923
TACAATA151300.031.3869922
ATATAGC149600.031.3115946
CGTATAC10400.030.50329225
TATAGCT153850.030.4320077
Produced by FastQC (version 0.11.5)