FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005204057

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005204057
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45791974
Sequences flagged as poor quality0
Sequence length35-51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTGAAGGCCGAAGTGGAGAAGGGTTCCATGTGAACAGCAGTTGAACATG808470.17655277319994983No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG519640.11347840125870091No Hit
CGAGAACTTTGAAGGCCGAAGTGGAGAAGGGTTCCATGTGAACAGCAGTTG473620.10342860519618569No Hit
CTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGAT463590.10123826502871443No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGACA75000.022.81997712
CGGACAC77500.022.19850213
TCACCCG79200.022.0255933
ACGGACA79750.021.92982918
AAACCGT80500.021.73386245
GGCGATC59800.020.9141252
CGGACAG84150.020.8882319
GGACACG86200.020.06073214
GACACGG90800.019.36165615
CACGGAC90800.019.26179117
CCCGGAC91350.019.0035711
ACACGGA95700.018.64843216
CACCCGG101950.017.3941634
CGAGAAC143700.016.1583581
TGCGCGA72250.016.1414511
CGCGAGT72150.016.0711613
GTCTAAC77700.015.8156761
GCCCGGA112500.015.66814410
ACGTGCG75550.015.5833528
CGAGTCG76900.015.19267215
Produced by FastQC (version 0.11.5)