FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005204070

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005204070
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16414004
Sequences flagged as poor quality0
Sequence length35-51
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCGTCCTGGGAAACGGGGCGCGGCCGGAAAGGCGGCCGCCCCCTCGCC2479831.5108013864258836No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2426771.478475331186711No Hit
GCCCTTCGTCCTGGGAAACGGGGCGCGGCCGGAAAGGCGGCCGCCCCCTCG1931041.1764588335667518No Hit
CTTCGATGTCGGCTCTTCCTATCATTGTGAAGCAGAATTCACCAAGCGTTG1702541.037248437370918No Hit
GGGAGTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCT1216970.7414217761857497No Hit
CCTTCGTCCTGGGAAACGGGGCGCGGCCGGAAAGGCGGCCGCCCCCTCGC1131430.689307739903073No Hit
CTCCCATCCAAGTACTAACCAGGCCCGACCCTGCTTAGCTTCCGAGATC1116320.6801021859139306No Hit
CAGAAAAGTTACCACAGGGATAACTGGCTTGTGGCGGCCAAGCGTTCAT1114920.6792492556965382No Hit
CTCCGAAGTTTCCCTCAGGATAGCTGGCGCTCTCGCAGACCCGACGCACC1029910.6274581144247315No Hit
GTCCGTCCTTCCGTTCGTCTTCCTCCCTCCCGGCCTCTCCCGCCGACCGCG823860.5019250635006547No Hit
GGTGTATGTGCTTGGCTGAGGAGCCAATGGGGCGAAGCTACCATCTGTGGG820700.4999998781528261No Hit
GTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTGCCTACC776410.4730168214897474No Hit
AGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAAGGCTTCCAT754740.4598146801962519No Hit
CTCGGGGCCACGCGCGCGTCCCCCGAAGAGGGGGACGGCGGAGCGAGCGC670530.40851092762009805No Hit
CTGATGATGTGTTGTTGCCATGGTAATCCTGCTCAGTACGAGAGGAACCG667150.40645171038096495No Hit
CTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGGCGAGCT611300.3724258870657032No Hit
GCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGG607260.3699645741526565No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT604860.36850240806569806No Hit
GGGGAGTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTC594650.36228210983742903No Hit
GGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA591980.3606554500656878No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG554500.3378212896743537No Hit
CTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCAGTACGAGAGGAA547890.33379424057652235No Hit
GTTGTTGCCATGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGA541040.32962097486999514No Hit
CTCGTAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGCC539730.3288228758808637No Hit
CTGGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCG531350.3237174792939005No Hit
CCCGCACTTCGACCTGAGCCACGGCTCTGCCCAGGTTAAGGGCCACGGCA512890.31247098514171195No Hit
GTCGCTTTTTGATCCTTCGATGTCGGCTCTTCCTATCATTGTGAAGCAGA510250.3108626024460577No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGAT501880.30576329821779014No Hit
GTGGCGGCCAAGCGTTCATAGCGACGTCGCTTTTTGATCCTTCGATGTCGG491980.2997318631090866No Hit
GTCAGAAAAGTTACCACAGGGATAACTGGCTTGTGGCGGCCAAGCGTTCAT445990.27171310546774574No Hit
GTTGCCATGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACATT442190.26939800916339485No Hit
CTCAGGATAGCTGGCGCTCTCGCAGACCCGACGCACCCCCGCCACGCAGTT431520.2628974624351255No Hit
CTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTG430340.2621785641090376No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG418520.25497739613076736No Hit
CCGAAGTTTCCCTCAGGATAGCTGGCGCTCTCGCAGACCCGACGCACCCC411410.250645729098153No Hit
CTGAGAGATGGGCGAGCGCCGTTCCGAAGGGACGGGCGATGGCCTCCGTTG403700.2459485205437991No Hit
GTGGGGAGTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGT395140.24073346150031402No Hit
CATGGAAGTCGGAATCCGCTAAGGAGTGTGTAACAACTCACCTGCCGAAT382840.23323986030465205No Hit
CCTGCCCTTTGTACACACCGCCCGTCGCTACTACCGATTGGATGGTTTAGT382290.23290478057639072No Hit
CCTAAGGCGAGCTCAGGGAGGACAGAAACCTCCCGTGGAGCAGAAGGGCA380960.23209449686986797No Hit
CTCTTTTCTTTGTGAAGGGCAGGGCGCCCTGGAATGGGTTCGCCCCGAG365470.22265743325029044No Hit
GGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAAGG364200.2218837036959416No Hit
GTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAATTCT357560.21783837752202326No Hit
CTTGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGG352600.21481656760897586No Hit
GTCGGCTCTTCCTATCATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTT347200.21152669391331938No Hit
GTGCTTGGCTGAGGAGCCAATGGGGCGAAGCTACCATCTGTGGGATTATG346240.21094182747853604No Hit
CTGCCCTTTGTACACACCGCCCGTCGCTACTACCGATTGGATGGTTTAGTG338690.20634209666331263No Hit
CTGGGTTTAGACCGTCGTGAGACAGGTTAGTTTTACCCTACTGATGATGTG338450.20619588005461675No Hit
GTCCTGAGAGATGGGCGAGCGCCGTTCCGAAGGGACGGGCGATGGCCTCCG335240.2042402329133099No Hit
CCTCAGGATAGCTGGCGCTCTCGCAGACCCGACGCACCCCCGCCACGCAGT324110.19745943768504018No Hit
CTGAAAATGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGG323470.1970695267285179No Hit
GTTGGATTGTTCACCCACTAATAGGGAACGTGAGCTGGGTTTAGACCGTCG322280.19634453604373434No Hit
TGATGATGTGTTGTTGCCATGGTAATCCTGCTCAGTACGAGAGGAACCGC317940.19370045236981787No Hit
GTGCCCTTCGTCCTGGGAAACGGGGCGCGGCCGGAAAGGCGGCCGCCCC314910.19185446768503284No Hit
GTTTTAAGCAGGAGGTGTCAGAAAAGTTACCACAGGGATAACTGGCTTGTG313720.1911294770002493No Hit
CGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGTATTAGAGGCAC313360.19091015208720555No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG312660.19048368697850934No Hit
GGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGAA309540.18858287106546337No Hit
GTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAAGGCTTC300160.18286823860893417No Hit
GTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA299900.182709837282847No Hit
GTCCTAAGGCGAGCTCAGGGAGGACAGAAACCTCCCGTGGAGCAGAAGGG298710.18198484659806344No Hit
CCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGTATTAGAGGC298150.1816436745111065No Hit
GTATGTGCTTGGCTGAGGAGCCAATGGGGCGAAGCTACCATCTGTGGGATT295120.1797976898263215No Hit
GTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGCCGGGGGCATT294700.17954181076110376No Hit
GCCGAAGTGGAGAAGGCTTCCATGTGAACAGCAGTTGAACATGGGTCAGT293790.1789874061197987No Hit
CCCACCACCACGCCCGCGGTCGGCGGGAGAGGCCGGGAGGGAGGAAGACG291920.17784813504371025No Hit
GCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTTTACCCTACTGATGATGT286700.17466792380457566No Hit
AGAAAAGTTACCACAGGGATAACTGGCTTGTGGCGGCCAAGCGTTCATAG284790.17350428329370457No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGT281310.17138414246761485No Hit
GGCGGCCAAGCGTTCATAGCGACGTCGCTTTTTGATCCTTCGATGTCGG279740.1704276421523962No Hit
CTCCTGCCGACAAGACCAACGTCAAGGCCGCCTGGGGTAAGGTCGGCGCG277140.1688436288915246No Hit
GCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGAACG274440.16719869204369636No Hit
GTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAAGG272310.16590101964152074No Hit
GTGGTAAACTCCATCTAAGGCTAAATACCGGCACGAGACCGATAGTCAAC270350.16470691733717135No Hit
GCTCGTAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGC267790.16314727351108238No Hit
CTTCCGTTCGTCTTCCTCCCTCCCGGCCTCTCCCGCCGACCGCGGGCGTGG264390.16107587155455794No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT261680.15942484234803403No Hit
CCTGTCCTTCCCCACCACCAAGACCTACTTCCCGCACTTCGACCTGAGCC258480.15747528756542278No Hit
TGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGGCGAGCTC251920.15347870026106977No Hit
GTCCTGGGAAACGGGGCGCGGCCGGAAAGGCGGCCGCCCCCTCGCCCGTC245850.14978063853280407No Hit
CCTGGCTTCTGTGAGCACCGTGCTGACCTCCAAATACCGTTAAGCTGGAG243140.14812960932628016No Hit
ATGTGCTTGGCTGAGGAGCCAATGGGGCGAAGCTACCATCTGTGGGATTAT241740.14727667910888775No Hit
GCTCTTTCTCGATTCCGTGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGG238080.14504687582627615No Hit
GTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGG237470.1446752419458409No Hit
GTCCACTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGC236190.1438954200327964No Hit
CGCCCGTCACGCACCGCACGTTCGTGGGGAACCTGGCGCTAAACCATTCGT235840.14368218747844827No Hit
CGACTGTTTAATTAAAACAAAGCATCGCGAAGGCCCGCGGCGGGTGTTGA235100.1432313529349694No Hit
GCCATGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACATTTGG234650.14295719679366473No Hit
CTTGGCTGAGGAGCCAATGGGGCGAAGCTACCATCTGTGGGATTATGA228850.13942362875018185No Hit
GGCGAAGCTACCATCTGTGGGATTATGACTGAACGCCTCTAAGTCAGAAT224750.1369257616849612No Hit
CTCCAAGGTGAACAGCCTCTGGCATGTTGGAACAATGTAGGTAAGGGAAGT223520.136176401565395No Hit
CGCATGAATGGATGAACGAGATTCCCACTGTCCCTACCTACTATCCAGCG222310.13543922616322013No Hit
GTGGCCATGGAAGTCGGAATCCGCTAAGGAGTGTGTAACAACTCACCTGC222250.1354026720110462No Hit
GTCCGTAGCGGTCCTGACGTGCAAATCGGTCGTCCGACCTGGGTATAGGGG220460.134312139804523No Hit
CTGGCTTCTGTGAGCACCGTGCTGACCTCCAAATACCGTTAAGCTGGAGC220010.1340379836632183No Hit
GCCCTTTGTACACACCGCCCGTCGCTACTACCGATTGGATGGTTTAGTG219010.1334287477936523No Hit
CGCGCATGAATGGATGAACGAGATTCCCACTGTCCCTACCTACTATCCAG218250.13296572853278213No Hit
CTCTAAGTCAGAATCCCGCCCAGGCGGAACGATACGGCAGCGCCGCGGAG215540.13131469932625822No Hit
GTCGGTCCTGAGAGATGGGCGAGCGCCGTTCCGAAGGGACGGGCGATGGC213900.13031555250016996No Hit
CCCTGGACAAGTTCCTGGCTTCTGTGAGCACCGTGCTGACCTCCAAATAC213090.1298220714458215No Hit
CTCACTGTCAACCCAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGT206890.12604480905451224No Hit
CCACGCCCGCGGTCGGCGGGAGAGGCCGGGAGGGAGGAAGACGAACGGA205390.1251309552501632No Hit
CTGGCATGTTGGAACAATGTAGGTAAGGGAAGTCGGCAAGCCGGATCCGT203500.12397949945668345No Hit
GGGAAGTCGGCAAGCCGGATCCGTAACTTCGGGATAAGGATTGGCTCTA202150.12315703103276934No Hit
CTGGCATGCTAACTAGTTACGCGACCCCCGAGCGGTCGGCGTCCCCCAA200550.1221822536414637No Hit
CCCCGATCAACCTCACCACCTCTTGCTCAGCCTATATACCGCCATCTTC200300.12202994467407222No Hit
CTTTTCTTTGTGAAGGGCAGGGCGCCCTGGAATGGGTTCGCCCCGAGAG199380.12146944767407147No Hit
TGTTGCCATGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACAT198180.12073836463059226No Hit
AGCGATTTGTCTGGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAA197360.12023879121754813No Hit
CGTCCTTCCGTTCGTCTTCCTCCCTCCCGGCCTCTCCCGCCGACCGCGGG196380.11964174006537345No Hit
GGAGGTGTCAGAAAAGTTACCACAGGGATAACTGGCTTGTGGCGGCCAAG192750.11743021385884883No Hit
CCACCACCACGCCCGCGGTCGGCGGGAGAGGCCGGGAGGGAGGAAGACGA190360.11597414013058605No Hit
CCCGTGCCTTGGAAAGCGTCGCGGTTCCGGCGGCGTCCGGTGAGCTCTCG189890.11568779927189003No Hit
CTCGATTCCGTGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGCGATT188470.1148226843371063No Hit
GGCTCATTAAATCAGTTATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTT187970.11451806640232329No Hit
CAGGTGGGGAGTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGT187940.11449978932623629No Hit
GGCGGAATCAGCGGGGAAAGAAGACCCTGTTGAGCTTGACTCTAGTCTGG187520.11424391026101859No Hit
GCCAAGCGTTCATAGCGACGTCGCTTTTTGATCCTTCGATGTCGGCTCTT186380.11354938136971333No Hit
CAGAAAAGGTGTTGGTTGATATAGACAGCAGGACGGTGGCCATGGAAGTCG186120.11339098004362616No Hit
GAGCGATTTGTCTGGTTAATTCCGATAACGAACGAGACTCTGGCATGCTA185040.11273300530449486No Hit
TGCCCTTCGTCCTGGGAAACGGGGCGCGGCCGGAAAGGCGGCCGCCCCCT183590.11184961329362415No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC182780.11135613223927569No Hit
CAGGTGTCCTAAGGCGAGCTCAGGGAGGACAGAAACCTCCCGTGGAGC181500.11057631032623119No Hit
ATGGAAGTCGGAATCCGCTAAGGAGTGTGTAACAACTCACCTGCCGAATC181350.1104849249457963No Hit
CTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGT180880.11019858408710026No Hit
CCGAAAGATGGTGAACTATGCCTGGGCAGGGCGAAGCCAGAGGAAACT180600.11002799804362177No Hit
CCGCACTTCGACCTGAGCCACGGCTCTGCCCAGGTTAAGGGCCACGGCA180490.1099609820979695No Hit
CCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGTATTAGAGGCA179810.10954670170666463No Hit
CGCCCTTCGTCCTGGGAAACGGGGCGCGGCCGGAAAGGCGGCCGCCCCCT179090.10910805188057711No Hit
GGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCA178680.10885826517405503No Hit
CACCACCACGCCCGCGGTCGGCGGGAGAGGCCGGGAGGGAGGAAGACGAA178410.10869377148927221No Hit
GGTTGATATAGACAGCAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGG176590.10758496220666207No Hit
CTCATTAAATCAGTTATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGG176010.10723160540231377No Hit
GTGTTGTTGCCATGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTC171150.10427071907622297No Hit
GTTCCTGTCCTTCCCCACCACCAAGACCTACTTCCCGCACTTCGACCTG170820.10406967123926618No Hit
ATCAACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACG167560.10208356230448098No Hit
CTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGACCACG167040.10176675965230665No Hit
GGGTAAACGGCGGGAGTAACTATGACTCTCTTAAGGTAGCCAAATGCCTCG166210.10126109388056685No Hit
GCCATGGAAGTCGGAATCCGCTAAGGAGTGTGTAACAACTCACCTGCCGA165960.10110878491317535No Hit
CCCTGTTGAGCTTGACTCTAGTCTGGCACGGTGAAGAGACATGAGAGGTGT164880.10045081017404406No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)