FastQCFastQC Report
Thu 16 May 2024
EGAF00005204074

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005204074
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences87958840
Total Bases8.8 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACTGTAGATCTCGTAT4703860.534779676494142TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACTGTAGATCGCGTAT1371640.1559411197328205TruSeq Adapter, Index 7 (97% over 35bp)
ACACTCTTTCCCTACACGACGCTCTTCCGATCAGATCGGAAGAGCACACG1173620.13342831715379602Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)