FastQCFastQC Report
Sun 5 May 2024
EGAF00005204078

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005204078
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56660040
Total Bases5.7 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACGTCGAGATCTCGTAT4281270.7556065968184985TruSeq Adapter, Index 15 (97% over 38bp)
ACACTCTTTCCCTACACGACGCTCTTCCGATCAGATCGGAAGAGCACACG3150030.5559526608170414Illumina Single End PCR Primer 1 (96% over 32bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACGTCGAGATCGCGTAT1381040.24374144458775535TruSeq Adapter, Index 15 (97% over 38bp)
CGCGGCTCCCAAACCACGCTCCCCGGACCCCGTCCCGGCCCGGAGCGGAC603240.10646656797277236No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)