FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005204106

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005204106
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59540155
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTAATAGAAATTGGACAGCAAGAAAGCGAGCTTAGTGATACTTGTGGGC3377910.5673330880646851No Hit
GTCTTGGTGGTGGGGAAGGACAGGAACATCCTCTCCAGGGCCTCCGCACCA2997440.5034316756481403No Hit
CACCAACTTCATCCACGTTCACCTTGCCCCACAGGGCAGTAACGGCAGACT2629750.4416767138076816No Hit
GGTCGAAGTGCGGGAAGTAGGTCTTGGTGGTGGGGAAGGACAGGAACATCC2592040.43534317302331516No Hit
GTCCAGGTGAGCCAGGCCATCACTAAAGGCACCGAGCACTTTCTTGCCATG2549260.42815810607144034No Hit
GTCACAGTGCAGCTCACTCAGTGTGGCAAAGGTGCCCTTGAGGTTGTCCAG2468030.4145152124645964No Hit
GCGGGAAGTAGGTCTTGGTGGTGGGGAAGGACAGGAACATCCTCTCCAGGG2387970.4010688248964081No Hit
GTCCACGTGCGCCACGGCGTTGGTCAGCGCGTCGGCCACCTTCTTGCCGTG2097800.35233364777098747No Hit
GGCATGTCGTCCACGTGCGCCACGGCGTTGGTCAGCGCGTCGGCCACCTTC2095610.35196582877555493No Hit
CTCAGGTCGAAGTGCGGGAAGTAGGTCTTGGTGGTGGGGAAGGACAGGAAC1997220.33544084660175977No Hit
CTCACCACCAACTTCATCCACGTTCACCTTGCCCCACAGGGCAGTAACGGC1976270.33192221283266726No Hit
GGGCATGTCGTCCACGTGCGCCACGGCGTTGGTCAGCGCGTCGGCCACCTT1931790.32445162428616453No Hit
ATCAGGAGTGGACAGATCCCCAAAGGACTCAAAGAACCTCTGGGTCCAAGG1900220.31914932032004284No Hit
CTTGCCATGAGCCTTCACCTTAGGGTTGCCCATAACAGCATCAGGAGTGGA1894260.3181483152000528No Hit
CTGGGCAGAGCCGTGGCTCAGGTCGAAGTGCGGGAAGTAGGTCTTGGTGGT1751030.2940922810832454No Hit
CCTTAGGGTTGCCCATAACAGCATCAGGAGTGGACAGATCCCCAAAGGACT1693620.2844500488787777No Hit
GACAGCAAGAAAGCGAGCTTAGTGATACTTGTGGGCCAGGGCATTAGCCAC1662420.2792098878479574No Hit
CTTAGGGTTGCCCATAACAGCATCAGGAGTGGACAGATCCCCAAAGGACTC1645880.27643193068610583No Hit
GCCCATAACAGCATCAGGAGTGGACAGATCCCCAAAGGACTCAAAGAACCT1636040.2747792645148472No Hit
GGACAGCAAGAAAGCGAGCTTAGTGATACTTGTGGGCCAGGGCATTAGCCA1587410.2666116673696936No Hit
CCTTGAGGTTGTCCAGGTGAGCCAGGCCATCACTAAAGGCACCGAGCACTT1585310.2662589642233884No Hit
GTCGAAGTGCGGGAAGTAGGTCTTGGTGGTGGGGAAGGACAGGAACATCCT1542410.2590537428060105No Hit
CCTGCACTGGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGA1533340.25753040112173037No Hit
GTCGTCCACGTGCGCCACGGCGTTGGTCAGCGCGTCGGCCACCTTCTTGCC1512440.2540201650465976No Hit
CAGCAAGAAAGCGAGCTTAGTGATACTTGTGGGCCAGGGCATTAGCCACAC1464100.24590127452640995No Hit
GCCTGCACTGGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAG1448800.2433315801747577No Hit
GGGAAGTAGGTCTTGGTGGTGGGGAAGGACAGGAACATCCTCTCCAGGGCC1350150.22676292999237235No Hit
GCACTTTCTTGCCATGAGCCTTCACCTTAGGGTTGCCCATAACAGCATCAG1328320.2230964968095901No Hit
GGCAGAATCCAGATGCTCAAGGCCCTTCATAATATCCCCCAGTTTAGTAGT1293610.2172668176628025No Hit
GTCCAGGGAGGCGTGCACCGCAGGGGTGAACTCGGCGGGGAGGTGGGCGGC1254940.21077204115441084No Hit
GTTGGACTTAGGGAACAAAGGAACCTTTAATAGAAATTGGACAGCAAGAAA1245220.2091395294486553No Hit
CTTGGTGGTGGGGAAGGACAGGAACATCCTCTCCAGGGCCTCCGCACCATA1214690.20401189751689425No Hit
GTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCC1195000.20070488563558492No Hit
CTGCACTGGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGAC1190830.20000451795935031No Hit
CTCCAGCTTAACGGTATTTGGAGGTCAGCACGGTGCTCACAGAAGCCAGGA1135380.19069147535810077No Hit
CTTCACCTTAGGGTTGCCCATAACAGCATCAGGAGTGGACAGATCCCCAAA1131590.19005493015595945No Hit
CTTGAGGTTGTCCAGGTGAGCCAGGCCATCACTAAAGGCACCGAGCACTTT1093370.1836357328932046No Hit
CTCTGGGTCCAAGGGTAGACCACCAGCAGCCTGCCCAGGGCCTCACCACCA1092710.18352488333293723No Hit
GTCAGCGCGTCGGCCACCTTCTTGCCGTGGCCCTTAACCTGGGCAGAGCCG1070690.17982653891310832No Hit
GCTTGTCACAGTGCAGCTCACTCAGTGTGGCAAAGGTGCCCTTGAGGTTGT1066850.17918159601700734No Hit
CCTGGGCAGAGCCGTGGCTCAGGTCGAAGTGCGGGAAGTAGGTCTTGGTGG1055620.17729547395367043No Hit
CCCAGGGCCTCACCACCAACTTCATCCACGTTCACCTTGCCCCACAGGGCA1046400.17574693918751136No Hit
GGGAGGAGGGCCCGTTGGGAGGCCCAGCGGGCAGGAGGAACGGCTACCGAG1043690.17529178417489844No Hit
GTCAGCACGGTGCTCACAGAAGCCAGGAACTTGTCCAGGGAGGCGTGCACC1016180.17067137295830015No Hit
CCCATAACAGCATCAGGAGTGGACAGATCCCCAAAGGACTCAAAGAACCTC997660.16756086711564658No Hit
CGGTATTTGGAGGTCAGCACGGTGCTCACAGAAGCCAGGAACTTGTCCAGG927850.15583600680918616No Hit
GGCCGGTGCAAGGAGGGGAGGAGGGCCCGTTGGGAGGCCCAGCGGGCAGGA913010.15334357124196266No Hit
GGTATTTGGAGGTCAGCACGGTGCTCACAGAAGCCAGGAACTTGTCCAGGG897360.15071509303259287No Hit
GCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGTGCAGGTCGCTCA881380.1480311900430894No Hit
GCCATCACTAAAGGCACCGAGCACTTTCTTGCCATGAGCCTTCACCTTAGG859420.14434292285601205No Hit
CGGGAAGTAGGTCTTGGTGGTGGGGAAGGACAGGAACATCCTCTCCAGGGC856470.1438474589123928No Hit
CATCAGGAGTGGACAGATCCCCAAAGGACTCAAAGAACCTCTGGGTCCAAG852240.14313701400340662No Hit
CCCCAGTTTAGTAGTTGGACTTAGGGAACAAAGGAACCTTTAATAGAAATT846110.14210745672395378No Hit
GGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCC838050.14075374845765853No Hit
GTTGGGCATGTCGTCCACGTGCGCCACGGCGTTGGTCAGCGCGTCGGCCAC832380.13980144996263447No Hit
CTCAGGATCCACGTGCAGCTTGTCACAGTGCAGCTCACTCAGTGTGGCAAA827750.13902382350197107No Hit
GTAGGTCTTGGTGGTGGGGAAGGACAGGAACATCCTCTCCAGGGCCTCCGC793240.1332277351310221No Hit
CTCGGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAG781080.13118541596003572No Hit
GGCCTCACCACCAACTTCATCCACGTTCACCTTGCCCCACAGGGCAGTAAC761050.12782129975980075No Hit
ACCAACTTCATCCACGTTCACCTTGCCCCACAGGGCAGTAACGGCAGACTT760490.12772724558745271No Hit
GGGGAGGAGGGCCCGTTGGGAGGCCCAGCGGGCAGGAGGAACGGCTACCGA744440.12503158582640572No Hit
CTTAGTGATACTTGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCT728650.12237959407394891No Hit
CACCACTTTCTGATAGGCAGCCTGCACTGGTGGGGTGAATTCTTTGCCAAA703230.11811020646486392No Hit
CTTGTCACAGTGCAGCTCACTCAGTGTGGCAAAGGTGCCCTTGAGGTTGTC701770.1178649938012422No Hit
GGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTTGAA700970.11773063069788785No Hit
ATTTGGAGGTCAGCACGGTGCTCACAGAAGCCAGGAACTTGTCCAGGGAGG695980.11689254084071499No Hit
GCTTAGTGATACTTGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTC689840.11586130402247022No Hit
GGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTG683040.11471921764395811No Hit
CTCAGTGTGGCAAAGGTGCCCTTGAGGTTGTCCAGGTGAGCCAGGCCATCA676600.11363759466195544No Hit
GGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTTGAAG658520.11060098852614676No Hit
CAGGAGTGGACAGATCCCCAAAGGACTCAAAGAACCTCTGGGTCCAAGGGT655460.11008704965581632No Hit
CGTCCACGTGCGCCACGGCGTTGGTCAGCGCGTCGGCCACCTTCTTGCCGT647900.10881731832911755No Hit
GTGCAGCTTGTCACAGTGCAGCTCACTCAGTGTGGCAAAGGTGCCCTTGAG646520.10858554197583127No Hit
GTGGGAAGTAGGTCTTGGTGGTGGGGAAGGACAGGAACATCCTCTCCAGGG635110.10666918821423962No Hit
GCAGAATCCAGATGCTCAAGGCCCTTCATAATATCCCCCAGTTTAGTAGTT631700.10609646548619164No Hit
AGCATCAGGAGTGGACAGATCCCCAAAGGACTCAAAGAACCTCTGGGTCCA628220.10551198598660012No Hit
AGCAAGAAAGCGAGCTTAGTGATACTTGTGGGCCAGGGCATTAGCCACACC627020.1053104413315686No Hit
GTGCGGGAAGTAGGTCTTGGTGGTGGGGAAGGACAGGAACATCCTCTCCAG602760.10123588022234742No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)